Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS13410 and BPHYT_RS13415 are separated by 26 nucleotides BPHYT_RS13415 and BPHYT_RS13420 are separated by 124 nucleotides BPHYT_RS13420 and BPHYT_RS13425 overlap by 4 nucleotides BPHYT_RS13425 and BPHYT_RS13430 are separated by 2 nucleotides
BPHYT_RS13410: BPHYT_RS13410 - FAD-dependent oxidoreductase, at 3,063,017 to 3,064,306
_RS13410
BPHYT_RS13415: BPHYT_RS13415 - hypothetical protein, at 3,064,333 to 3,064,617
_RS13415
BPHYT_RS13420: BPHYT_RS13420 - hypothetical protein, at 3,064,742 to 3,065,725
_RS13420
BPHYT_RS13425: BPHYT_RS13425 - histone acetyltransferase, at 3,065,722 to 3,066,333
_RS13425
BPHYT_RS13430: BPHYT_RS13430 - radical SAM protein, at 3,066,336 to 3,067,406
_RS13430
Position (kb)
3064
3065
3066 Strain fitness (log2 ratio)
-2
-1
0
1 at 3064.033 kb on - strand, within BPHYT_RS13410 at 3064.267 kb on - strand at 3064.515 kb on - strand, within BPHYT_RS13415 at 3064.886 kb on + strand, within BPHYT_RS13420 at 3065.059 kb on + strand, within BPHYT_RS13420 at 3065.451 kb on + strand, within BPHYT_RS13420 at 3065.455 kb on + strand, within BPHYT_RS13420 at 3066.151 kb on - strand, within BPHYT_RS13425 at 3066.347 kb on - strand at 3066.607 kb on + strand, within BPHYT_RS13430 at 3066.665 kb on - strand, within BPHYT_RS13430
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 3,064,033 - BPHYT_RS13410 0.79 -0.4 3,064,267 - +0.2 3,064,515 - BPHYT_RS13415 0.64 -2.0 3,064,886 + BPHYT_RS13420 0.15 -0.9 3,065,059 + BPHYT_RS13420 0.32 -0.2 3,065,451 + BPHYT_RS13420 0.72 +0.2 3,065,455 + BPHYT_RS13420 0.72 -1.7 3,066,151 - BPHYT_RS13425 0.70 +0.5 3,066,347 - -2.2 3,066,607 + BPHYT_RS13430 0.25 +0.3 3,066,665 - BPHYT_RS13430 0.31 +0.8
Or see this region's nucleotide sequence