Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS13385 and BPHYT_RS13390 are separated by 158 nucleotides BPHYT_RS13390 and BPHYT_RS13395 are separated by 53 nucleotides BPHYT_RS13395 and BPHYT_RS13400 are separated by 137 nucleotides
BPHYT_RS13385: BPHYT_RS13385 - hypothetical protein, at 3,057,548 to 3,058,756
_RS13385
BPHYT_RS13390: BPHYT_RS13390 - IclR family transcriptional regulator, at 3,058,915 to 3,059,745
_RS13390
BPHYT_RS13395: BPHYT_RS13395 - endoribonuclease L-PSP, at 3,059,799 to 3,060,260
_RS13395
BPHYT_RS13400: BPHYT_RS13400 - hypothetical protein, at 3,060,398 to 3,060,724
_RS13400
Position (kb)
3058
3059
3060 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3058.281 kb on + strand, within BPHYT_RS13385 at 3058.407 kb on + strand, within BPHYT_RS13385 at 3058.408 kb on - strand, within BPHYT_RS13385 at 3058.731 kb on - strand at 3058.823 kb on + strand at 3058.823 kb on + strand at 3058.824 kb on - strand at 3058.824 kb on - strand at 3058.824 kb on - strand at 3058.824 kb on - strand at 3059.209 kb on + strand, within BPHYT_RS13390 at 3059.743 kb on + strand at 3059.744 kb on - strand at 3060.158 kb on - strand, within BPHYT_RS13395
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 3,058,281 + BPHYT_RS13385 0.61 +1.4 3,058,407 + BPHYT_RS13385 0.71 +0.7 3,058,408 - BPHYT_RS13385 0.71 -0.6 3,058,731 - -2.4 3,058,823 + -0.3 3,058,823 + +0.8 3,058,824 - -1.1 3,058,824 - +1.4 3,058,824 - -0.5 3,058,824 - +1.8 3,059,209 + BPHYT_RS13390 0.35 +1.5 3,059,743 + -0.5 3,059,744 - +0.3 3,060,158 - BPHYT_RS13395 0.78 -0.2
Or see this region's nucleotide sequence