Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS13105 and BPHYT_RS13110 are separated by 153 nucleotides BPHYT_RS13110 and BPHYT_RS13115 overlap by 4 nucleotides BPHYT_RS13115 and BPHYT_RS13120 are separated by 47 nucleotides
BPHYT_RS13105: BPHYT_RS13105 - LacI family transcription regulator, at 2,994,558 to 2,995,628
_RS13105
BPHYT_RS13110: BPHYT_RS13110 - ABC transporter substrate-binding protein, at 2,995,782 to 2,996,870
_RS13110
BPHYT_RS13115: BPHYT_RS13115 - ABC transporter permease, at 2,996,867 to 2,997,691
_RS13115
BPHYT_RS13120: BPHYT_RS13120 - ABC transporter permease, at 2,997,739 to 2,998,641
_RS13120
Position (kb)
2995
2996
2997 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2994.801 kb on - strand, within BPHYT_RS13105 at 2994.985 kb on - strand, within BPHYT_RS13105 at 2995.072 kb on + strand, within BPHYT_RS13105 at 2995.595 kb on + strand at 2995.596 kb on - strand at 2995.658 kb on - strand at 2996.103 kb on + strand, within BPHYT_RS13110 at 2996.104 kb on - strand, within BPHYT_RS13110 at 2996.104 kb on - strand, within BPHYT_RS13110 at 2996.158 kb on - strand, within BPHYT_RS13110 at 2996.211 kb on + strand, within BPHYT_RS13110 at 2996.317 kb on - strand, within BPHYT_RS13110 at 2996.484 kb on + strand, within BPHYT_RS13110 at 2996.485 kb on - strand, within BPHYT_RS13110 at 2996.784 kb on + strand at 2997.029 kb on + strand, within BPHYT_RS13115 at 2997.030 kb on - strand, within BPHYT_RS13115 at 2997.560 kb on + strand, within BPHYT_RS13115 at 2997.560 kb on + strand, within BPHYT_RS13115 at 2997.561 kb on - strand, within BPHYT_RS13115
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 2,994,801 - BPHYT_RS13105 0.23 -0.3 2,994,985 - BPHYT_RS13105 0.40 -1.1 2,995,072 + BPHYT_RS13105 0.48 -0.5 2,995,595 + -1.0 2,995,596 - -0.1 2,995,658 - +1.0 2,996,103 + BPHYT_RS13110 0.29 +0.5 2,996,104 - BPHYT_RS13110 0.30 +0.1 2,996,104 - BPHYT_RS13110 0.30 -0.5 2,996,158 - BPHYT_RS13110 0.35 +1.0 2,996,211 + BPHYT_RS13110 0.39 +1.3 2,996,317 - BPHYT_RS13110 0.49 -1.0 2,996,484 + BPHYT_RS13110 0.64 +2.5 2,996,485 - BPHYT_RS13110 0.65 -1.8 2,996,784 + +1.3 2,997,029 + BPHYT_RS13115 0.20 +0.2 2,997,030 - BPHYT_RS13115 0.20 +0.8 2,997,560 + BPHYT_RS13115 0.84 -0.1 2,997,560 + BPHYT_RS13115 0.84 +1.0 2,997,561 - BPHYT_RS13115 0.84 +0.3
Or see this region's nucleotide sequence