Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS12910 and BPHYT_RS12915 are separated by 622 nucleotides BPHYT_RS12915 and BPHYT_RS12920 are separated by 89 nucleotides BPHYT_RS12920 and BPHYT_RS12925 are separated by 170 nucleotides BPHYT_RS12925 and BPHYT_RS12930 are separated by 21 nucleotides
BPHYT_RS12910: BPHYT_RS12910 - phage portal protein, lambda family, at 2,947,675 to 2,948,058
_RS12910
BPHYT_RS12915: BPHYT_RS12915 - tRNA-Met, at 2,948,681 to 2,948,757
_RS12915
BPHYT_RS12920: BPHYT_RS12920 - hypothetical protein, at 2,948,847 to 2,949,134
_RS12920
BPHYT_RS12925: BPHYT_RS12925 - exodeoxyribonuclease III, at 2,949,305 to 2,950,081
_RS12925
BPHYT_RS12930: BPHYT_RS12930 - peptidase S9, at 2,950,103 to 2,952,202
_RS12930
Position (kb)
2948
2949
2950 Strain fitness (log2 ratio)
-1
0
1 at 2948.105 kb on + strand at 2948.264 kb on + strand at 2948.390 kb on + strand at 2948.462 kb on + strand at 2948.651 kb on - strand at 2948.666 kb on - strand at 2948.776 kb on - strand at 2948.777 kb on + strand at 2948.902 kb on + strand, within BPHYT_RS12920 at 2949.182 kb on - strand at 2949.489 kb on - strand, within BPHYT_RS12925 at 2950.123 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 2,948,105 + -0.2 2,948,264 + +0.3 2,948,390 + +0.5 2,948,462 + +0.4 2,948,651 - +0.7 2,948,666 - +1.0 2,948,776 - +0.1 2,948,777 + -0.6 2,948,902 + BPHYT_RS12920 0.19 -0.8 2,949,182 - -0.4 2,949,489 - BPHYT_RS12925 0.24 -1.5 2,950,123 + +0.9
Or see this region's nucleotide sequence