Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS12835 and BPHYT_RS12840 are separated by 87 nucleotides BPHYT_RS12840 and BPHYT_RS12845 are separated by 275 nucleotides
BPHYT_RS12835: BPHYT_RS12835 - dihydrolipoamide acetyltransferase, at 2,924,895 to 2,926,547
_RS12835
BPHYT_RS12840: BPHYT_RS12840 - pyruvate dehydrogenase, at 2,926,635 to 2,929,331
_RS12840
BPHYT_RS12845: BPHYT_RS12845 - ATPase, at 2,929,607 to 2,932,123
_RS12845
Position (kb)
2926
2927
2928
2929
2930 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 2925.705 kb on - strand, within BPHYT_RS12835 at 2926.637 kb on - strand at 2926.637 kb on - strand at 2927.252 kb on - strand, within BPHYT_RS12840 at 2927.586 kb on - strand, within BPHYT_RS12840 at 2927.586 kb on - strand, within BPHYT_RS12840 at 2927.586 kb on - strand, within BPHYT_RS12840 at 2927.674 kb on + strand, within BPHYT_RS12840 at 2928.202 kb on + strand, within BPHYT_RS12840 at 2928.203 kb on - strand, within BPHYT_RS12840 at 2928.680 kb on - strand at 2928.680 kb on - strand, within BPHYT_RS12840 at 2928.866 kb on - strand, within BPHYT_RS12840 at 2929.130 kb on - strand at 2929.300 kb on + strand at 2929.348 kb on + strand at 2929.349 kb on - strand at 2929.412 kb on + strand at 2929.544 kb on - strand at 2929.578 kb on + strand at 2929.721 kb on - strand at 2929.822 kb on + strand at 2929.822 kb on + strand at 2929.979 kb on + strand, within BPHYT_RS12845 at 2929.980 kb on - strand, within BPHYT_RS12845 at 2929.980 kb on - strand, within BPHYT_RS12845 at 2929.980 kb on - strand at 2929.980 kb on - strand, within BPHYT_RS12845 at 2929.980 kb on - strand, within BPHYT_RS12845 at 2929.980 kb on - strand, within BPHYT_RS12845 at 2929.992 kb on - strand, within BPHYT_RS12845 at 2929.992 kb on - strand, within BPHYT_RS12845 at 2930.117 kb on - strand, within BPHYT_RS12845 at 2930.147 kb on + strand, within BPHYT_RS12845 at 2930.147 kb on + strand, within BPHYT_RS12845 at 2930.148 kb on - strand, within BPHYT_RS12845 at 2930.148 kb on - strand, within BPHYT_RS12845 at 2930.169 kb on - strand, within BPHYT_RS12845
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 2,925,705 - BPHYT_RS12835 0.49 +1.4 2,926,637 - +0.1 2,926,637 - -2.2 2,927,252 - BPHYT_RS12840 0.23 +0.4 2,927,586 - BPHYT_RS12840 0.35 +2.4 2,927,586 - BPHYT_RS12840 0.35 -0.2 2,927,586 - BPHYT_RS12840 0.35 -1.7 2,927,674 + BPHYT_RS12840 0.39 -1.3 2,928,202 + BPHYT_RS12840 0.58 +0.4 2,928,203 - BPHYT_RS12840 0.58 -0.6 2,928,680 - +0.4 2,928,680 - BPHYT_RS12840 0.76 -0.4 2,928,866 - BPHYT_RS12840 0.83 -0.7 2,929,130 - -0.6 2,929,300 + -2.8 2,929,348 + +0.1 2,929,349 - +0.3 2,929,412 + -2.3 2,929,544 - -0.3 2,929,578 + +2.2 2,929,721 - -2.4 2,929,822 + -4.2 2,929,822 + -2.1 2,929,979 + BPHYT_RS12845 0.15 -3.8 2,929,980 - BPHYT_RS12845 0.15 +0.1 2,929,980 - BPHYT_RS12845 0.15 -4.8 2,929,980 - +0.1 2,929,980 - BPHYT_RS12845 0.15 -4.6 2,929,980 - BPHYT_RS12845 0.15 -3.8 2,929,980 - BPHYT_RS12845 0.15 +0.4 2,929,992 - BPHYT_RS12845 0.15 -3.0 2,929,992 - BPHYT_RS12845 0.15 -2.9 2,930,117 - BPHYT_RS12845 0.20 -1.6 2,930,147 + BPHYT_RS12845 0.21 -2.3 2,930,147 + BPHYT_RS12845 0.21 -4.5 2,930,148 - BPHYT_RS12845 0.21 -3.2 2,930,148 - BPHYT_RS12845 0.21 -3.6 2,930,169 - BPHYT_RS12845 0.22 -3.4
Or see this region's nucleotide sequence