Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS12640 and BPHYT_RS12645 are separated by 119 nucleotides BPHYT_RS12645 and BPHYT_RS12650 are separated by 83 nucleotides
BPHYT_RS12640: BPHYT_RS12640 - phosphoesterase, at 2,883,556 to 2,884,386
_RS12640
BPHYT_RS12645: BPHYT_RS12645 - alpha/beta hydrolase, at 2,884,506 to 2,885,390
_RS12645
BPHYT_RS12650: BPHYT_RS12650 - hypothetical protein, at 2,885,474 to 2,886,403
_RS12650
Position (kb)
2884
2885
2886 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2883.695 kb on + strand, within BPHYT_RS12640 at 2883.801 kb on - strand, within BPHYT_RS12640 at 2883.892 kb on + strand, within BPHYT_RS12640 at 2883.893 kb on - strand, within BPHYT_RS12640 at 2883.893 kb on - strand, within BPHYT_RS12640 at 2883.897 kb on - strand, within BPHYT_RS12640 at 2884.013 kb on - strand, within BPHYT_RS12640 at 2884.013 kb on - strand, within BPHYT_RS12640 at 2884.016 kb on + strand, within BPHYT_RS12640 at 2884.017 kb on - strand, within BPHYT_RS12640 at 2884.017 kb on - strand, within BPHYT_RS12640 at 2884.017 kb on - strand, within BPHYT_RS12640 at 2884.017 kb on - strand, within BPHYT_RS12640 at 2884.017 kb on - strand, within BPHYT_RS12640 at 2884.017 kb on - strand, within BPHYT_RS12640 at 2884.017 kb on - strand, within BPHYT_RS12640 at 2884.017 kb on - strand, within BPHYT_RS12640 at 2884.017 kb on - strand, within BPHYT_RS12640 at 2884.164 kb on + strand, within BPHYT_RS12640 at 2884.164 kb on + strand, within BPHYT_RS12640 at 2884.215 kb on - strand, within BPHYT_RS12640 at 2884.215 kb on - strand, within BPHYT_RS12640 at 2884.418 kb on - strand at 2884.430 kb on + strand at 2884.446 kb on + strand at 2884.447 kb on - strand at 2884.447 kb on - strand at 2884.447 kb on - strand at 2884.447 kb on - strand at 2884.507 kb on + strand at 2884.708 kb on - strand, within BPHYT_RS12645 at 2884.708 kb on - strand, within BPHYT_RS12645 at 2884.745 kb on - strand, within BPHYT_RS12645 at 2884.907 kb on - strand at 2884.907 kb on - strand, within BPHYT_RS12645 at 2884.961 kb on + strand, within BPHYT_RS12645 at 2885.144 kb on - strand, within BPHYT_RS12645 at 2885.158 kb on + strand, within BPHYT_RS12645 at 2885.158 kb on + strand, within BPHYT_RS12645 at 2885.159 kb on - strand, within BPHYT_RS12645 at 2885.436 kb on - strand at 2885.604 kb on + strand, within BPHYT_RS12650 at 2885.625 kb on + strand, within BPHYT_RS12650 at 2885.625 kb on + strand, within BPHYT_RS12650 at 2885.910 kb on + strand, within BPHYT_RS12650 at 2885.983 kb on - strand, within BPHYT_RS12650 at 2886.226 kb on - strand, within BPHYT_RS12650
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 2,883,695 + BPHYT_RS12640 0.17 -0.3 2,883,801 - BPHYT_RS12640 0.29 +0.9 2,883,892 + BPHYT_RS12640 0.40 +2.4 2,883,893 - BPHYT_RS12640 0.41 -0.0 2,883,893 - BPHYT_RS12640 0.41 +1.8 2,883,897 - BPHYT_RS12640 0.41 +0.6 2,884,013 - BPHYT_RS12640 0.55 +1.0 2,884,013 - BPHYT_RS12640 0.55 +1.2 2,884,016 + BPHYT_RS12640 0.55 -0.7 2,884,017 - BPHYT_RS12640 0.55 +0.7 2,884,017 - BPHYT_RS12640 0.55 +0.8 2,884,017 - BPHYT_RS12640 0.55 +1.4 2,884,017 - BPHYT_RS12640 0.55 -1.3 2,884,017 - BPHYT_RS12640 0.55 +1.4 2,884,017 - BPHYT_RS12640 0.55 +1.4 2,884,017 - BPHYT_RS12640 0.55 +4.6 2,884,017 - BPHYT_RS12640 0.55 +0.0 2,884,017 - BPHYT_RS12640 0.55 +0.6 2,884,164 + BPHYT_RS12640 0.73 +1.1 2,884,164 + BPHYT_RS12640 0.73 -1.2 2,884,215 - BPHYT_RS12640 0.79 +1.5 2,884,215 - BPHYT_RS12640 0.79 +0.5 2,884,418 - -0.4 2,884,430 + +1.5 2,884,446 + +0.4 2,884,447 - -0.7 2,884,447 - +0.3 2,884,447 - -0.2 2,884,447 - -0.2 2,884,507 + -0.7 2,884,708 - BPHYT_RS12645 0.23 +1.0 2,884,708 - BPHYT_RS12645 0.23 -2.1 2,884,745 - BPHYT_RS12645 0.27 -1.3 2,884,907 - -1.4 2,884,907 - BPHYT_RS12645 0.45 +1.0 2,884,961 + BPHYT_RS12645 0.51 -0.3 2,885,144 - BPHYT_RS12645 0.72 +0.9 2,885,158 + BPHYT_RS12645 0.74 +0.8 2,885,158 + BPHYT_RS12645 0.74 -0.1 2,885,159 - BPHYT_RS12645 0.74 -0.0 2,885,436 - +1.6 2,885,604 + BPHYT_RS12650 0.14 -1.0 2,885,625 + BPHYT_RS12650 0.16 -0.7 2,885,625 + BPHYT_RS12650 0.16 -1.4 2,885,910 + BPHYT_RS12650 0.47 -1.4 2,885,983 - BPHYT_RS12650 0.55 -0.9 2,886,226 - BPHYT_RS12650 0.81 -0.6
Or see this region's nucleotide sequence