Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS12525 and BPHYT_RS12530 are separated by 204 nucleotides BPHYT_RS12530 and BPHYT_RS12535 are separated by 281 nucleotides BPHYT_RS12535 and BPHYT_RS12540 are separated by 44 nucleotides
BPHYT_RS12525: BPHYT_RS12525 - adenylosuccinate synthetase, at 2,859,313 to 2,860,659
_RS12525
BPHYT_RS12530: BPHYT_RS12530 - ATP phosphoribosyltransferase regulatory subunit, at 2,860,864 to 2,862,015
_RS12530
BPHYT_RS12535: BPHYT_RS12535 - membrane protein, at 2,862,297 to 2,862,488
_RS12535
BPHYT_RS12540: BPHYT_RS12540 - membrane protein, at 2,862,533 to 2,863,435
_RS12540
Position (kb)
2860
2861
2862
2863 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2860.692 kb on - strand at 2860.696 kb on - strand at 2860.740 kb on - strand at 2860.796 kb on + strand at 2861.222 kb on + strand, within BPHYT_RS12530 at 2861.688 kb on + strand, within BPHYT_RS12530 at 2861.709 kb on - strand, within BPHYT_RS12530 at 2861.874 kb on + strand, within BPHYT_RS12530 at 2861.875 kb on - strand, within BPHYT_RS12530 at 2861.875 kb on - strand, within BPHYT_RS12530 at 2861.875 kb on - strand, within BPHYT_RS12530 at 2861.875 kb on - strand at 2861.876 kb on + strand, within BPHYT_RS12530 at 2862.001 kb on + strand at 2862.001 kb on + strand at 2862.001 kb on + strand at 2862.028 kb on - strand at 2862.382 kb on - strand, within BPHYT_RS12535 at 2862.496 kb on - strand at 2862.587 kb on - strand at 2862.676 kb on - strand, within BPHYT_RS12540
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 2,860,692 - -0.2 2,860,696 - -1.0 2,860,740 - +0.2 2,860,796 + -0.3 2,861,222 + BPHYT_RS12530 0.31 -0.1 2,861,688 + BPHYT_RS12530 0.72 -3.2 2,861,709 - BPHYT_RS12530 0.73 -4.2 2,861,874 + BPHYT_RS12530 0.88 -5.5 2,861,875 - BPHYT_RS12530 0.88 -5.4 2,861,875 - BPHYT_RS12530 0.88 -2.6 2,861,875 - BPHYT_RS12530 0.88 -2.6 2,861,875 - -1.1 2,861,876 + BPHYT_RS12530 0.88 -4.5 2,862,001 + -3.5 2,862,001 + -0.7 2,862,001 + -1.4 2,862,028 - -1.3 2,862,382 - BPHYT_RS12535 0.44 +0.3 2,862,496 - -0.0 2,862,587 - -0.3 2,862,676 - BPHYT_RS12540 0.16 -1.3
Or see this region's nucleotide sequence