Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS12475 and BPHYT_RS12480 overlap by 14 nucleotides BPHYT_RS12480 and BPHYT_RS12485 overlap by 21 nucleotides BPHYT_RS12485 and BPHYT_RS12490 are separated by 32 nucleotides
BPHYT_RS12475: BPHYT_RS12475 - DNA mismatch repair protein MutS, at 2,843,553 to 2,846,237
_RS12475
BPHYT_RS12480: BPHYT_RS12480 - hypothetical protein, at 2,846,224 to 2,847,582
_RS12480
BPHYT_RS12485: BPHYT_RS12485 - peptidyl-prolyl cis-trans isomerase, at 2,847,562 to 2,848,104
_RS12485
BPHYT_RS12490: BPHYT_RS12490 - cupin, at 2,848,137 to 2,849,387
_RS12490
Position (kb)
2846
2847
2848 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6 at 2845.509 kb on + strand, within BPHYT_RS12475 at 2845.510 kb on - strand, within BPHYT_RS12475 at 2845.521 kb on + strand, within BPHYT_RS12475 at 2845.521 kb on + strand, within BPHYT_RS12475 at 2845.522 kb on - strand, within BPHYT_RS12475 at 2845.773 kb on - strand, within BPHYT_RS12475 at 2845.777 kb on + strand, within BPHYT_RS12475 at 2845.777 kb on + strand, within BPHYT_RS12475 at 2846.365 kb on + strand, within BPHYT_RS12480 at 2846.383 kb on + strand at 2846.492 kb on - strand, within BPHYT_RS12480 at 2846.695 kb on - strand, within BPHYT_RS12480 at 2846.695 kb on - strand, within BPHYT_RS12480 at 2846.834 kb on - strand, within BPHYT_RS12480 at 2847.222 kb on + strand, within BPHYT_RS12480 at 2847.896 kb on + strand, within BPHYT_RS12485 at 2847.897 kb on - strand, within BPHYT_RS12485 at 2847.995 kb on + strand, within BPHYT_RS12485 at 2847.996 kb on - strand, within BPHYT_RS12485 at 2848.010 kb on + strand, within BPHYT_RS12485 at 2848.010 kb on + strand, within BPHYT_RS12485 at 2848.067 kb on + strand at 2848.068 kb on - strand at 2848.120 kb on - strand at 2848.132 kb on - strand at 2848.133 kb on + strand at 2848.133 kb on + strand at 2848.134 kb on - strand at 2848.134 kb on - strand at 2848.134 kb on - strand at 2848.134 kb on - strand at 2848.421 kb on + strand, within BPHYT_RS12490 at 2848.422 kb on - strand, within BPHYT_RS12490
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 2,845,509 + BPHYT_RS12475 0.73 -0.2 2,845,510 - BPHYT_RS12475 0.73 +2.2 2,845,521 + BPHYT_RS12475 0.73 +5.4 2,845,521 + BPHYT_RS12475 0.73 +4.4 2,845,522 - BPHYT_RS12475 0.73 -1.2 2,845,773 - BPHYT_RS12475 0.83 +2.3 2,845,777 + BPHYT_RS12475 0.83 +0.7 2,845,777 + BPHYT_RS12475 0.83 +5.9 2,846,365 + BPHYT_RS12480 0.10 -0.1 2,846,383 + +2.4 2,846,492 - BPHYT_RS12480 0.20 +1.1 2,846,695 - BPHYT_RS12480 0.35 -0.4 2,846,695 - BPHYT_RS12480 0.35 -0.1 2,846,834 - BPHYT_RS12480 0.45 -0.4 2,847,222 + BPHYT_RS12480 0.73 -0.9 2,847,896 + BPHYT_RS12485 0.62 +0.1 2,847,897 - BPHYT_RS12485 0.62 +0.1 2,847,995 + BPHYT_RS12485 0.80 +0.1 2,847,996 - BPHYT_RS12485 0.80 -1.3 2,848,010 + BPHYT_RS12485 0.83 +1.3 2,848,010 + BPHYT_RS12485 0.83 -2.1 2,848,067 + -0.2 2,848,068 - +0.5 2,848,120 - -0.5 2,848,132 - +0.5 2,848,133 + -0.2 2,848,133 + -0.6 2,848,134 - -0.9 2,848,134 - -0.4 2,848,134 - -0.9 2,848,134 - +1.1 2,848,421 + BPHYT_RS12490 0.23 -0.3 2,848,422 - BPHYT_RS12490 0.23 +0.8
Or see this region's nucleotide sequence