Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS12330 and BPHYT_RS12335 are separated by 94 nucleotides BPHYT_RS12335 and BPHYT_RS12340 are separated by 61 nucleotides BPHYT_RS12340 and BPHYT_RS12345 are separated by 97 nucleotides BPHYT_RS12345 and BPHYT_RS12350 are separated by 155 nucleotides BPHYT_RS12350 and BPHYT_RS12355 are separated by 122 nucleotides BPHYT_RS12355 and BPHYT_RS12360 are separated by 406 nucleotides
BPHYT_RS12330: BPHYT_RS12330 - tRNA-Asp, at 2,814,906 to 2,814,982
_RS12330
BPHYT_RS12335: BPHYT_RS12335 - tRNA-Glu, at 2,815,077 to 2,815,152
_RS12335
BPHYT_RS12340: BPHYT_RS12340 - tRNA-Asp, at 2,815,214 to 2,815,290
_RS12340
BPHYT_RS12345: BPHYT_RS12345 - tRNA-Glu, at 2,815,388 to 2,815,463
_RS12345
BPHYT_RS12350: BPHYT_RS12350 - tRNA-Ala, at 2,815,619 to 2,815,694
_RS12350
BPHYT_RS12355: BPHYT_RS12355 - glutamate--tRNA ligase, at 2,815,817 to 2,817,226
_RS12355
BPHYT_RS12360: BPHYT_RS12360 - esterase, at 2,817,633 to 2,818,586
_RS12360
Position (kb)
2815
2816
2817
2818 Strain fitness (log2 ratio)
-2
-1
0
1 at 2814.840 kb on + strand at 2814.865 kb on + strand at 2815.088 kb on - strand, within BPHYT_RS12335 at 2815.165 kb on - strand at 2817.359 kb on - strand at 2817.359 kb on - strand at 2817.360 kb on + strand at 2817.379 kb on - strand at 2817.492 kb on + strand at 2817.492 kb on + strand at 2817.492 kb on + strand at 2817.492 kb on + strand at 2817.493 kb on - strand at 2817.493 kb on - strand at 2817.520 kb on + strand at 2817.521 kb on - strand at 2817.734 kb on + strand, within BPHYT_RS12360 at 2817.734 kb on + strand, within BPHYT_RS12360 at 2817.735 kb on - strand, within BPHYT_RS12360 at 2817.735 kb on - strand, within BPHYT_RS12360 at 2817.939 kb on + strand, within BPHYT_RS12360 at 2817.940 kb on - strand, within BPHYT_RS12360 at 2817.940 kb on - strand, within BPHYT_RS12360 at 2818.066 kb on - strand, within BPHYT_RS12360 at 2818.176 kb on - strand, within BPHYT_RS12360
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 2,814,840 + +0.3 2,814,865 + +0.9 2,815,088 - BPHYT_RS12335 0.14 +0.5 2,815,165 - +0.5 2,817,359 - -0.8 2,817,359 - +0.3 2,817,360 + -0.8 2,817,379 - +0.5 2,817,492 + -0.5 2,817,492 + -1.2 2,817,492 + -0.1 2,817,492 + -0.5 2,817,493 - -0.2 2,817,493 - -1.3 2,817,520 + -0.7 2,817,521 - -0.1 2,817,734 + BPHYT_RS12360 0.11 -0.2 2,817,734 + BPHYT_RS12360 0.11 +0.1 2,817,735 - BPHYT_RS12360 0.11 -0.7 2,817,735 - BPHYT_RS12360 0.11 -2.3 2,817,939 + BPHYT_RS12360 0.32 +1.0 2,817,940 - BPHYT_RS12360 0.32 -0.8 2,817,940 - BPHYT_RS12360 0.32 +0.1 2,818,066 - BPHYT_RS12360 0.45 -0.2 2,818,176 - BPHYT_RS12360 0.57 +0.7
Or see this region's nucleotide sequence