Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS12065 and BPHYT_RS12070 are separated by 73 nucleotides BPHYT_RS12070 and BPHYT_RS12075 overlap by 4 nucleotides BPHYT_RS12075 and BPHYT_RS12080 are separated by 3 nucleotides BPHYT_RS12080 and BPHYT_RS12085 are separated by 128 nucleotides
BPHYT_RS12065: BPHYT_RS12065 - RNA methyltransferase, at 2,750,697 to 2,751,482
_RS12065
BPHYT_RS12070: BPHYT_RS12070 - ribonuclease HII, at 2,751,556 to 2,752,302
_RS12070
BPHYT_RS12075: BPHYT_RS12075 - lipid-A-disaccharide synthase, at 2,752,299 to 2,753,468
_RS12075
BPHYT_RS12080: BPHYT_RS12080 - acyl-[acyl-carrier-protein]--UDP-N- acetylglucosamine O-acyltransferase, at 2,753,472 to 2,754,260
_RS12080
BPHYT_RS12085: BPHYT_RS12085 - 3-hydroxyacyl-ACP dehydratase, at 2,754,389 to 2,754,853
_RS12085
Position (kb)
2752
2753
2754 Strain fitness (log2 ratio)
-2
-1
0
1 at 2751.404 kb on + strand at 2751.404 kb on + strand at 2751.404 kb on + strand at 2751.404 kb on + strand at 2751.448 kb on + strand at 2751.449 kb on - strand at 2751.535 kb on + strand at 2751.536 kb on - strand at 2752.040 kb on + strand, within BPHYT_RS12070 at 2752.040 kb on + strand, within BPHYT_RS12070 at 2752.100 kb on - strand, within BPHYT_RS12070 at 2752.276 kb on - strand at 2752.276 kb on - strand at 2754.391 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 2,751,404 + +0.2 2,751,404 + -0.9 2,751,404 + -0.4 2,751,404 + +0.9 2,751,448 + +0.2 2,751,449 - +0.3 2,751,535 + -0.1 2,751,536 - -0.2 2,752,040 + BPHYT_RS12070 0.65 -0.2 2,752,040 + BPHYT_RS12070 0.65 +0.2 2,752,100 - BPHYT_RS12070 0.73 +0.0 2,752,276 - -2.4 2,752,276 - -0.0 2,754,391 - -0.4
Or see this region's nucleotide sequence