Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS12055

Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
2,745,849	+		BPHYT_RS12045	0.71	+0.2
2,745,849	+		BPHYT_RS12045	0.71	+0.2
2,745,892	+		BPHYT_RS12045	0.76	-0.4
2,745,892	+		BPHYT_RS12045	0.76	-0.5
2,745,940	+		BPHYT_RS12045	0.81	-0.1
2,745,942	+		BPHYT_RS12045	0.81	+0.8
2,745,943	-		BPHYT_RS12045	0.81	+2.6
2,745,994	+		BPHYT_RS12045	0.87	-2.6
2,745,995	-		BPHYT_RS12045	0.87	-1.6
2,746,118	+				+0.0
2,746,119	-				-0.3
2,746,144	+				-0.1
2,746,144	+				+0.1
2,746,558	+		BPHYT_RS12050	0.83	-1.0
2,746,558	+		BPHYT_RS12050	0.83	-0.7
2,746,667	+				+0.3
2,746,680	-				-0.2
2,746,680	-				+0.3
2,746,680	-				-0.5
2,746,751	-				-0.9
2,746,765	+				-0.1
2,746,943	+				-3.4
2,746,943	+				-1.9
2,746,943	+				-2.8
2,746,943	+				-3.9
2,746,944	-				-1.6
2,746,944	-				-0.9
2,747,351	+		BPHYT_RS12055	0.23	-6.5
2,747,351	+		BPHYT_RS12055	0.23	-4.8
2,747,352	-		BPHYT_RS12055	0.23	-2.5
2,747,429	+		BPHYT_RS12055	0.26	-2.9
2,747,429	+		BPHYT_RS12055	0.26	-3.9
2,747,429	+		BPHYT_RS12055	0.26	-3.4
2,747,430	-		BPHYT_RS12055	0.26	-5.2
2,747,477	+		BPHYT_RS12055	0.28	-3.0
2,747,478	-		BPHYT_RS12055	0.28	-1.1
2,747,801	+		BPHYT_RS12055	0.42	-3.7
2,747,801	+		BPHYT_RS12055	0.42	-4.1
2,747,802	-		BPHYT_RS12055	0.42	-4.3
2,747,802	-		BPHYT_RS12055	0.42	-2.3
2,747,802	-		BPHYT_RS12055	0.42	-4.4
2,747,802	-		BPHYT_RS12055	0.42	-3.7
2,747,802	-		BPHYT_RS12055	0.42	-1.1
2,747,916	+		BPHYT_RS12055	0.46	-2.2
2,747,916	+		BPHYT_RS12055	0.46	-2.8
2,748,258	-		BPHYT_RS12055	0.61	-3.8
2,748,282	-		BPHYT_RS12055	0.62	-3.1
2,748,461	+		BPHYT_RS12055	0.69	-3.2
2,748,462	-		BPHYT_RS12055	0.69	-2.5
2,748,462	-		BPHYT_RS12055	0.69	-5.3
2,748,467	+		BPHYT_RS12055	0.69	-2.8
2,748,467	+		BPHYT_RS12055	0.69	-2.6
2,748,467	+		BPHYT_RS12055	0.69	-1.3
2,748,468	-		BPHYT_RS12055	0.69	-0.1
2,748,468	-		BPHYT_RS12055	0.69	-2.0
2,748,518	+		BPHYT_RS12055	0.71	-5.4
2,748,519	-		BPHYT_RS12055	0.71	-4.7
2,748,519	-		BPHYT_RS12055	0.71	-5.2
2,748,519	-		BPHYT_RS12055	0.71	-3.1
2,748,668	+		BPHYT_RS12055	0.78	-2.7
2,748,676	-		BPHYT_RS12055	0.78	-4.6
2,748,676	-		BPHYT_RS12055	0.78	-3.5
2,748,689	+		BPHYT_RS12055	0.78	-2.2
2,748,689	+		BPHYT_RS12055	0.78	-3.1
2,748,689	+		BPHYT_RS12055	0.78	-3.7
2,748,690	-		BPHYT_RS12055	0.79	-3.6
2,748,690	-		BPHYT_RS12055	0.79	-6.5
2,748,690	-		BPHYT_RS12055	0.79	-3.0
2,748,690	-		BPHYT_RS12055	0.79	+0.6
2,748,690	-		BPHYT_RS12055	0.79	-4.3
2,748,690	-		BPHYT_RS12055	0.79	-1.2
2,748,752	+		BPHYT_RS12055	0.81	-2.4
2,748,753	-		BPHYT_RS12055	0.81	-2.4
2,748,753	-		BPHYT_RS12055	0.81	-4.9
2,748,753	-		BPHYT_RS12055	0.81	-5.1
2,749,165	+				-1.2
2,749,165	+				-6.2
2,749,165	+				-0.8
2,749,166	-				-2.2
2,749,166	-				-3.2
2,749,166	-				-5.0
2,749,166	-				-3.1
2,749,166	-				-1.6
2,749,166	-				-5.3
2,749,169	+				-3.2
2,749,169	+				-3.5
2,749,170	-				-4.0
2,749,170	-				-6.4
2,749,170	-				-0.9
2,749,170	-				-3.3
2,749,170	-				-4.2
2,749,170	-				-4.7
2,749,170	-				-5.0
2,749,170	-				-3.0
2,749,176	+				-1.7
2,749,206	+				-5.0
2,749,206	+				-3.9
2,749,206	+				-3.0
2,749,207	-				-4.5
2,749,207	-				-5.0
2,749,207	-				-4.2
2,749,207	-				-2.7
2,749,207	-				-2.8
2,749,279	+				-2.1
2,749,353	+				-2.4
2,749,483	-				+0.0
2,749,487	+				-0.6
2,749,734	+				-0.7
2,750,078	+		BPHYT_RS12060	0.44	-2.4
2,750,078	+		BPHYT_RS12060	0.44	+3.5