Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS11385 and BPHYT_RS11390 are separated by 375 nucleotides BPHYT_RS11390 and BPHYT_RS11395 are separated by 491 nucleotides
BPHYT_RS11385: BPHYT_RS11385 - lipid A biosynthesis lauroyl acyltransferase, at 2,586,281 to 2,587,159
_RS11385
BPHYT_RS11390: BPHYT_RS11390 - penicillin-binding protein 1A, at 2,587,535 to 2,590,036
_RS11390
BPHYT_RS11395: BPHYT_RS11395 - hypothetical protein, at 2,590,528 to 2,591,172
_RS11395
Position (kb)
2587
2588
2589
2590
2591 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 2586.742 kb on + strand, within BPHYT_RS11385 at 2586.774 kb on + strand, within BPHYT_RS11385 at 2586.927 kb on + strand, within BPHYT_RS11385 at 2586.988 kb on + strand, within BPHYT_RS11385 at 2587.001 kb on + strand, within BPHYT_RS11385 at 2587.002 kb on - strand, within BPHYT_RS11385 at 2587.093 kb on + strand at 2587.354 kb on + strand at 2587.727 kb on - strand at 2587.729 kb on - strand at 2587.812 kb on + strand, within BPHYT_RS11390 at 2587.813 kb on - strand, within BPHYT_RS11390 at 2587.881 kb on + strand, within BPHYT_RS11390 at 2588.050 kb on - strand, within BPHYT_RS11390 at 2588.154 kb on + strand, within BPHYT_RS11390 at 2588.377 kb on + strand, within BPHYT_RS11390 at 2588.377 kb on + strand, within BPHYT_RS11390 at 2588.377 kb on + strand, within BPHYT_RS11390 at 2588.378 kb on - strand, within BPHYT_RS11390 at 2588.378 kb on - strand, within BPHYT_RS11390 at 2588.378 kb on - strand, within BPHYT_RS11390 at 2588.452 kb on - strand, within BPHYT_RS11390 at 2588.905 kb on + strand, within BPHYT_RS11390 at 2589.070 kb on - strand, within BPHYT_RS11390 at 2589.070 kb on - strand at 2589.085 kb on - strand, within BPHYT_RS11390 at 2589.085 kb on - strand, within BPHYT_RS11390 at 2589.093 kb on + strand, within BPHYT_RS11390 at 2589.123 kb on + strand, within BPHYT_RS11390 at 2589.123 kb on + strand, within BPHYT_RS11390 at 2589.210 kb on + strand, within BPHYT_RS11390 at 2589.211 kb on - strand, within BPHYT_RS11390 at 2589.285 kb on + strand, within BPHYT_RS11390 at 2589.409 kb on - strand, within BPHYT_RS11390 at 2589.423 kb on + strand, within BPHYT_RS11390 at 2589.424 kb on - strand, within BPHYT_RS11390 at 2589.487 kb on - strand, within BPHYT_RS11390 at 2589.501 kb on + strand, within BPHYT_RS11390 at 2589.648 kb on + strand, within BPHYT_RS11390 at 2589.757 kb on + strand, within BPHYT_RS11390 at 2589.758 kb on - strand, within BPHYT_RS11390 at 2589.760 kb on - strand, within BPHYT_RS11390 at 2589.975 kb on + strand at 2590.045 kb on + strand at 2590.195 kb on + strand at 2590.195 kb on + strand at 2590.431 kb on + strand at 2590.452 kb on + strand at 2590.514 kb on + strand at 2590.514 kb on + strand at 2590.515 kb on - strand at 2590.528 kb on - strand at 2590.534 kb on - strand at 2590.890 kb on + strand, within BPHYT_RS11395 at 2590.891 kb on - strand, within BPHYT_RS11395
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 2,586,742 + BPHYT_RS11385 0.52 +0.8 2,586,774 + BPHYT_RS11385 0.56 -0.3 2,586,927 + BPHYT_RS11385 0.73 -1.1 2,586,988 + BPHYT_RS11385 0.80 +1.2 2,587,001 + BPHYT_RS11385 0.82 +0.1 2,587,002 - BPHYT_RS11385 0.82 +0.5 2,587,093 + +0.7 2,587,354 + +0.0 2,587,727 - -2.3 2,587,729 - -4.8 2,587,812 + BPHYT_RS11390 0.11 -2.3 2,587,813 - BPHYT_RS11390 0.11 -3.5 2,587,881 + BPHYT_RS11390 0.14 -3.0 2,588,050 - BPHYT_RS11390 0.21 -0.9 2,588,154 + BPHYT_RS11390 0.25 -3.3 2,588,377 + BPHYT_RS11390 0.34 +0.1 2,588,377 + BPHYT_RS11390 0.34 -1.2 2,588,377 + BPHYT_RS11390 0.34 -1.4 2,588,378 - BPHYT_RS11390 0.34 -3.7 2,588,378 - BPHYT_RS11390 0.34 -2.4 2,588,378 - BPHYT_RS11390 0.34 -3.3 2,588,452 - BPHYT_RS11390 0.37 -2.1 2,588,905 + BPHYT_RS11390 0.55 -3.1 2,589,070 - BPHYT_RS11390 0.61 -5.1 2,589,070 - +2.4 2,589,085 - BPHYT_RS11390 0.62 -5.5 2,589,085 - BPHYT_RS11390 0.62 -2.2 2,589,093 + BPHYT_RS11390 0.62 -0.6 2,589,123 + BPHYT_RS11390 0.63 -3.2 2,589,123 + BPHYT_RS11390 0.63 -2.0 2,589,210 + BPHYT_RS11390 0.67 -0.9 2,589,211 - BPHYT_RS11390 0.67 -2.7 2,589,285 + BPHYT_RS11390 0.70 -3.0 2,589,409 - BPHYT_RS11390 0.75 -2.7 2,589,423 + BPHYT_RS11390 0.75 -4.5 2,589,424 - BPHYT_RS11390 0.75 -2.4 2,589,487 - BPHYT_RS11390 0.78 -0.6 2,589,501 + BPHYT_RS11390 0.79 -3.9 2,589,648 + BPHYT_RS11390 0.84 -4.3 2,589,757 + BPHYT_RS11390 0.89 -2.0 2,589,758 - BPHYT_RS11390 0.89 -2.7 2,589,760 - BPHYT_RS11390 0.89 -1.1 2,589,975 + -2.2 2,590,045 + -1.3 2,590,195 + -1.0 2,590,195 + -0.8 2,590,431 + +1.0 2,590,452 + -0.7 2,590,514 + +0.4 2,590,514 + +1.5 2,590,515 - -0.3 2,590,528 - +1.8 2,590,534 - -1.7 2,590,890 + BPHYT_RS11395 0.56 -0.7 2,590,891 - BPHYT_RS11395 0.56 -0.2
Or see this region's nucleotide sequence