Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS11050 and BPHYT_RS11055 are separated by 39 nucleotides BPHYT_RS11055 and BPHYT_RS11060 are separated by 133 nucleotides BPHYT_RS11060 and BPHYT_RS11065 are separated by 241 nucleotides
BPHYT_RS11050: BPHYT_RS11050 - molecular chaperone, at 2,509,287 to 2,510,537
_RS11050
BPHYT_RS11055: BPHYT_RS11055 - cobyric acid synthase, at 2,510,577 to 2,511,341
_RS11055
BPHYT_RS11060: BPHYT_RS11060 - 4-hydroxybenzoate 3-monooxygenase, at 2,511,475 to 2,512,653
_RS11060
BPHYT_RS11065: BPHYT_RS11065 - threonine dehydratase, at 2,512,895 to 2,514,445
_RS11065
Position (kb)
2511
2512
2513 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 2510.854 kb on + strand, within BPHYT_RS11055 at 2510.974 kb on + strand, within BPHYT_RS11055 at 2510.974 kb on + strand, within BPHYT_RS11055 at 2510.975 kb on - strand, within BPHYT_RS11055 at 2511.205 kb on + strand, within BPHYT_RS11055 at 2511.205 kb on + strand, within BPHYT_RS11055 at 2511.248 kb on + strand, within BPHYT_RS11055 at 2511.288 kb on + strand at 2511.289 kb on - strand at 2511.289 kb on - strand at 2511.419 kb on - strand at 2511.425 kb on - strand at 2511.504 kb on - strand at 2511.789 kb on + strand, within BPHYT_RS11060 at 2512.750 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 2,510,854 + BPHYT_RS11055 0.36 -0.6 2,510,974 + BPHYT_RS11055 0.52 -0.2 2,510,974 + BPHYT_RS11055 0.52 +0.6 2,510,975 - BPHYT_RS11055 0.52 +0.6 2,511,205 + BPHYT_RS11055 0.82 -1.1 2,511,205 + BPHYT_RS11055 0.82 +0.1 2,511,248 + BPHYT_RS11055 0.88 +4.0 2,511,288 + -1.3 2,511,289 - +1.6 2,511,289 - -0.7 2,511,419 - +1.0 2,511,425 - -0.2 2,511,504 - +1.2 2,511,789 + BPHYT_RS11060 0.27 +1.0 2,512,750 + -0.4
Or see this region's nucleotide sequence