Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS10940 and BPHYT_RS10945 are separated by 40 nucleotides BPHYT_RS10945 and BPHYT_RS10950 are separated by 123 nucleotides BPHYT_RS10950 and BPHYT_RS10955 are separated by 31 nucleotides
BPHYT_RS10940: BPHYT_RS10940 - 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase, at 2,489,968 to 2,490,804
_RS10940
BPHYT_RS10945: BPHYT_RS10945 - 2-deoxy-D-gluconate 3-dehydrogenase, at 2,490,845 to 2,491,615
_RS10945
BPHYT_RS10950: BPHYT_RS10950 - transcriptional regulator, at 2,491,739 to 2,492,611
_RS10950
BPHYT_RS10955: BPHYT_RS10955 - dioxygenase, at 2,492,643 to 2,493,575
_RS10955
Position (kb)
2491
2492
2493 Strain fitness (log2 ratio)
-1
0
1
2 at 2491.325 kb on + strand, within BPHYT_RS10945 at 2491.422 kb on - strand, within BPHYT_RS10945 at 2491.579 kb on + strand at 2491.580 kb on - strand at 2491.580 kb on - strand at 2491.683 kb on + strand at 2492.150 kb on + strand, within BPHYT_RS10950 at 2492.151 kb on - strand, within BPHYT_RS10950 at 2492.221 kb on + strand, within BPHYT_RS10950 at 2492.222 kb on - strand, within BPHYT_RS10950 at 2492.628 kb on + strand at 2492.883 kb on + strand, within BPHYT_RS10955 at 2493.311 kb on - strand, within BPHYT_RS10955 at 2493.579 kb on - strand at 2493.579 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 2,491,325 + BPHYT_RS10945 0.62 -1.2 2,491,422 - BPHYT_RS10945 0.75 +0.4 2,491,579 + +2.0 2,491,580 - +0.3 2,491,580 - +2.4 2,491,683 + +0.9 2,492,150 + BPHYT_RS10950 0.47 -0.1 2,492,151 - BPHYT_RS10950 0.47 +0.8 2,492,221 + BPHYT_RS10950 0.55 +0.4 2,492,222 - BPHYT_RS10950 0.55 +0.2 2,492,628 + -0.3 2,492,883 + BPHYT_RS10955 0.26 +0.4 2,493,311 - BPHYT_RS10955 0.72 +1.0 2,493,579 - -1.4 2,493,579 - +1.8
Or see this region's nucleotide sequence