Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS10805 and BPHYT_RS10810 are separated by 93 nucleotides BPHYT_RS10810 and BPHYT_RS10815 are separated by 45 nucleotides BPHYT_RS10815 and BPHYT_RS10820 are separated by 3 nucleotides
BPHYT_RS10805: BPHYT_RS10805 - MFS transporter, at 2,455,585 to 2,456,961
_RS10805
BPHYT_RS10810: BPHYT_RS10810 - glucose-methanol-choline oxidoreductase, at 2,457,055 to 2,458,788
_RS10810
BPHYT_RS10815: BPHYT_RS10815 - crotonase, at 2,458,834 to 2,459,640
_RS10815
BPHYT_RS10820: BPHYT_RS10820 - 4-chlorobenzoate--CoA ligase, at 2,459,644 to 2,461,221
_RS10820
Position (kb)
2457
2458
2459 Strain fitness (log2 ratio)
-1
0
1
2 at 2456.130 kb on - strand, within BPHYT_RS10805 at 2456.423 kb on + strand, within BPHYT_RS10805 at 2456.496 kb on - strand, within BPHYT_RS10805 at 2456.768 kb on + strand, within BPHYT_RS10805 at 2456.768 kb on + strand, within BPHYT_RS10805 at 2457.047 kb on - strand at 2457.219 kb on - strand at 2457.767 kb on + strand, within BPHYT_RS10810 at 2457.768 kb on - strand, within BPHYT_RS10810 at 2458.325 kb on + strand, within BPHYT_RS10810 at 2458.325 kb on + strand, within BPHYT_RS10810 at 2458.507 kb on + strand, within BPHYT_RS10810 at 2458.773 kb on - strand at 2458.804 kb on - strand at 2458.804 kb on - strand at 2458.919 kb on + strand, within BPHYT_RS10815 at 2458.919 kb on + strand, within BPHYT_RS10815 at 2459.756 kb on - strand at 2459.774 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 2,456,130 - BPHYT_RS10805 0.40 +2.2 2,456,423 + BPHYT_RS10805 0.61 -0.6 2,456,496 - BPHYT_RS10805 0.66 +0.3 2,456,768 + BPHYT_RS10805 0.86 -0.3 2,456,768 + BPHYT_RS10805 0.86 -1.1 2,457,047 - -0.1 2,457,219 - +0.0 2,457,767 + BPHYT_RS10810 0.41 +0.2 2,457,768 - BPHYT_RS10810 0.41 -1.4 2,458,325 + BPHYT_RS10810 0.73 -0.0 2,458,325 + BPHYT_RS10810 0.73 -1.3 2,458,507 + BPHYT_RS10810 0.84 -0.2 2,458,773 - +0.3 2,458,804 - -0.7 2,458,804 - +1.0 2,458,919 + BPHYT_RS10815 0.11 +0.0 2,458,919 + BPHYT_RS10815 0.11 -1.2 2,459,756 - +0.6 2,459,774 + -0.8
Or see this region's nucleotide sequence