Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS09755

Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS09750 and BPHYT_RS09755 are separated by 79 nucleotidesBPHYT_RS09755 and BPHYT_RS09760 are separated by 129 nucleotides BPHYT_RS09750: BPHYT_RS09750 - glycosyl transferase, at 2,211,501 to 2,212,730 _RS09750 BPHYT_RS09755: BPHYT_RS09755 - glycosyl transferase family 1, at 2,212,810 to 2,213,976 _RS09755 BPHYT_RS09760: BPHYT_RS09760 - GDP-D-mannose dehydratase, at 2,214,106 to 2,215,149 _RS09760 Position (kb) 2212 2213 2214Strain fitness (log2 ratio) -2 -1 0 1at 2211.852 kb on + strand, within BPHYT_RS09750at 2211.853 kb on - strand, within BPHYT_RS09750at 2211.905 kb on + strand, within BPHYT_RS09750at 2211.905 kb on + strand, within BPHYT_RS09750at 2211.940 kb on - strand, within BPHYT_RS09750at 2212.035 kb on + strand, within BPHYT_RS09750at 2212.888 kb on + strandat 2212.889 kb on - strandat 2212.889 kb on - strandat 2212.889 kb on - strandat 2213.104 kb on + strand, within BPHYT_RS09755at 2213.106 kb on + strand, within BPHYT_RS09755at 2214.036 kb on + strandat 2214.064 kb on + strandat 2214.065 kb on - strandat 2214.647 kb on + strand, within BPHYT_RS09760at 2214.773 kb on + strand, within BPHYT_RS09760

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
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2,211,852 + BPHYT_RS09750 0.29 -1.2
2,211,853 - BPHYT_RS09750 0.29 -0.5
2,211,905 + BPHYT_RS09750 0.33 +0.3
2,211,905 + BPHYT_RS09750 0.33 +0.4
2,211,940 - BPHYT_RS09750 0.36 -0.1
2,212,035 + BPHYT_RS09750 0.43 -0.5
2,212,888 + -0.1
2,212,889 - +0.2
2,212,889 - +0.3
2,212,889 - -1.8
2,213,104 + BPHYT_RS09755 0.25 +1.1
2,213,106 + BPHYT_RS09755 0.25 +1.0
2,214,036 + -0.1
2,214,064 + -0.8
2,214,065 - -0.4
2,214,647 + BPHYT_RS09760 0.52 -1.0
2,214,773 + BPHYT_RS09760 0.64 -1.7

Or see this region's nucleotide sequence