Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS09680 and BPHYT_RS09685 are separated by 182 nucleotides BPHYT_RS09685 and BPHYT_RS09690 are separated by 235 nucleotides BPHYT_RS09690 and BPHYT_RS09695 are separated by 446 nucleotides
BPHYT_RS09680: BPHYT_RS09680 - hypothetical protein, at 2,194,498 to 2,194,692
_RS09680
BPHYT_RS09685: BPHYT_RS09685 - membrane protein, at 2,194,875 to 2,195,324
_RS09685
BPHYT_RS09690: BPHYT_RS09690 - phosphate starvation-inducible protein PsiF, at 2,195,560 to 2,195,859
_RS09690
BPHYT_RS09695: BPHYT_RS09695 - hypothetical protein, at 2,196,306 to 2,196,602
_RS09695
Position (kb)
2195
2196 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2194.945 kb on - strand, within BPHYT_RS09685 at 2195.008 kb on + strand, within BPHYT_RS09685 at 2195.009 kb on - strand, within BPHYT_RS09685 at 2195.009 kb on - strand, within BPHYT_RS09685 at 2195.038 kb on + strand, within BPHYT_RS09685 at 2195.039 kb on - strand, within BPHYT_RS09685 at 2195.212 kb on + strand, within BPHYT_RS09685 at 2195.320 kb on - strand at 2195.378 kb on - strand at 2195.385 kb on + strand at 2195.385 kb on + strand at 2195.758 kb on - strand, within BPHYT_RS09690 at 2195.920 kb on - strand at 2196.129 kb on + strand at 2196.229 kb on + strand at 2196.238 kb on + strand at 2196.313 kb on - strand at 2196.324 kb on + strand at 2196.324 kb on + strand at 2196.457 kb on + strand, within BPHYT_RS09695 at 2196.517 kb on - strand, within BPHYT_RS09695 at 2196.622 kb on - strand at 2196.698 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 2,194,945 - BPHYT_RS09685 0.16 -1.2 2,195,008 + BPHYT_RS09685 0.30 -0.7 2,195,009 - BPHYT_RS09685 0.30 -0.2 2,195,009 - BPHYT_RS09685 0.30 -0.4 2,195,038 + BPHYT_RS09685 0.36 -1.1 2,195,039 - BPHYT_RS09685 0.36 -0.5 2,195,212 + BPHYT_RS09685 0.75 -3.1 2,195,320 - +0.1 2,195,378 - +0.3 2,195,385 + +0.6 2,195,385 + -0.8 2,195,758 - BPHYT_RS09690 0.66 +2.1 2,195,920 - -0.7 2,196,129 + +1.4 2,196,229 + -0.3 2,196,238 + +0.2 2,196,313 - -2.7 2,196,324 + -1.1 2,196,324 + -1.3 2,196,457 + BPHYT_RS09695 0.51 -0.0 2,196,517 - BPHYT_RS09695 0.71 -0.2 2,196,622 - -0.2 2,196,698 - +0.4
Or see this region's nucleotide sequence