Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS09515 and BPHYT_RS09520 are separated by 331 nucleotides BPHYT_RS09520 and BPHYT_RS09525 overlap by 11 nucleotides BPHYT_RS09525 and BPHYT_RS09530 are separated by 75 nucleotides BPHYT_RS09530 and BPHYT_RS09535 are separated by 11 nucleotides
BPHYT_RS09515: BPHYT_RS09515 - phosphoribosylformylglycinamidine synthase, at 2,157,020 to 2,161,105
_RS09515
BPHYT_RS09520: BPHYT_RS09520 - XRE family transcriptional regulator, at 2,161,437 to 2,161,721
_RS09520
BPHYT_RS09525: BPHYT_RS09525 - toxin HipA, at 2,161,711 to 2,163,003
_RS09525
BPHYT_RS09530: BPHYT_RS09530 - peptidyl-prolyl cis-trans isomerase, at 2,163,079 to 2,163,858
_RS09530
BPHYT_RS09535: BPHYT_RS09535 - BolA family transcriptional regulator, at 2,163,870 to 2,164,190
_RS09535
Position (kb)
2161
2162
2163
2164 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5 at 2160.816 kb on - strand at 2161.128 kb on + strand at 2161.240 kb on + strand at 2161.241 kb on - strand at 2161.706 kb on + strand at 2161.707 kb on - strand at 2161.795 kb on + strand at 2162.384 kb on - strand, within BPHYT_RS09525 at 2162.485 kb on + strand, within BPHYT_RS09525 at 2162.665 kb on + strand, within BPHYT_RS09525 at 2162.704 kb on + strand, within BPHYT_RS09525 at 2163.905 kb on - strand, within BPHYT_RS09535 at 2163.961 kb on + strand, within BPHYT_RS09535 at 2163.961 kb on + strand, within BPHYT_RS09535 at 2163.962 kb on - strand, within BPHYT_RS09535
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 2,160,816 - +2.6 2,161,128 + +0.5 2,161,240 + +0.9 2,161,241 - -0.1 2,161,706 + +0.8 2,161,707 - -0.5 2,161,795 + -1.3 2,162,384 - BPHYT_RS09525 0.52 +0.1 2,162,485 + BPHYT_RS09525 0.60 +0.6 2,162,665 + BPHYT_RS09525 0.74 +4.9 2,162,704 + BPHYT_RS09525 0.77 +0.2 2,163,905 - BPHYT_RS09535 0.11 -1.7 2,163,961 + BPHYT_RS09535 0.28 +0.1 2,163,961 + BPHYT_RS09535 0.28 -0.1 2,163,962 - BPHYT_RS09535 0.29 +0.2
Or see this region's nucleotide sequence