Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS08660 and BPHYT_RS08665 are separated by 149 nucleotides BPHYT_RS08665 and BPHYT_RS08670 are separated by 125 nucleotides BPHYT_RS08670 and BPHYT_RS08675 are separated by 57 nucleotides BPHYT_RS08675 and BPHYT_RS08680 are separated by 148 nucleotides BPHYT_RS08680 and BPHYT_RS08685 are separated by 316 nucleotides
BPHYT_RS08660: BPHYT_RS08660 - hypothetical protein, at 1,972,548 to 1,972,829
_RS08660
BPHYT_RS08665: BPHYT_RS08665 - hypothetical protein, at 1,972,979 to 1,973,176
_RS08665
BPHYT_RS08670: BPHYT_RS08670 - hypothetical protein, at 1,973,302 to 1,973,574
_RS08670
BPHYT_RS08675: BPHYT_RS08675 - bleomycin resistance protein, at 1,973,632 to 1,974,000
_RS08675
BPHYT_RS08680: BPHYT_RS08680 - hypothetical protein, at 1,974,149 to 1,974,415
_RS08680
BPHYT_RS08685: BPHYT_RS08685 - hypothetical protein, at 1,974,732 to 1,974,956
_RS08685
Position (kb) 1973
1974
1975 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6
7
8 at 1972.829 kb on + strand at 1972.947 kb on - strand at 1972.947 kb on - strand at 1973.153 kb on + strand, within BPHYT_RS08665 at 1973.353 kb on + strand, within BPHYT_RS08670 at 1973.354 kb on - strand, within BPHYT_RS08670 at 1973.455 kb on - strand, within BPHYT_RS08670 at 1973.465 kb on + strand, within BPHYT_RS08670 at 1973.465 kb on + strand, within BPHYT_RS08670 at 1973.466 kb on - strand, within BPHYT_RS08670 at 1973.715 kb on + strand, within BPHYT_RS08675 at 1973.716 kb on - strand, within BPHYT_RS08675 at 1973.864 kb on + strand, within BPHYT_RS08675 at 1973.865 kb on - strand, within BPHYT_RS08675 at 1973.865 kb on - strand, within BPHYT_RS08675 at 1973.890 kb on + strand, within BPHYT_RS08675 at 1973.896 kb on + strand, within BPHYT_RS08675 at 1973.897 kb on - strand, within BPHYT_RS08675 at 1974.068 kb on + strand at 1974.322 kb on - strand, within BPHYT_RS08680 at 1974.507 kb on + strand at 1974.507 kb on + strand at 1974.519 kb on - strand at 1974.548 kb on + strand at 1974.677 kb on + strand at 1974.822 kb on - strand, within BPHYT_RS08685 at 1974.861 kb on + strand, within BPHYT_RS08685 at 1974.861 kb on + strand, within BPHYT_RS08685 at 1974.862 kb on - strand, within BPHYT_RS08685 at 1974.862 kb on - strand, within BPHYT_RS08685 at 1974.925 kb on + strand, within BPHYT_RS08685 at 1974.926 kb on - strand, within BPHYT_RS08685
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 1,972,829 + -1.7 1,972,947 - -1.1 1,972,947 - -0.5 1,973,153 + BPHYT_RS08665 0.88 +0.1 1,973,353 + BPHYT_RS08670 0.19 -0.2 1,973,354 - BPHYT_RS08670 0.19 +0.4 1,973,455 - BPHYT_RS08670 0.56 +1.0 1,973,465 + BPHYT_RS08670 0.60 -0.6 1,973,465 + BPHYT_RS08670 0.60 +0.4 1,973,466 - BPHYT_RS08670 0.60 +1.0 1,973,715 + BPHYT_RS08675 0.22 -0.5 1,973,716 - BPHYT_RS08675 0.23 -0.6 1,973,864 + BPHYT_RS08675 0.63 -0.9 1,973,865 - BPHYT_RS08675 0.63 +1.5 1,973,865 - BPHYT_RS08675 0.63 +8.7 1,973,890 + BPHYT_RS08675 0.70 +0.1 1,973,896 + BPHYT_RS08675 0.72 -0.2 1,973,897 - BPHYT_RS08675 0.72 -0.0 1,974,068 + +0.1 1,974,322 - BPHYT_RS08680 0.65 -0.1 1,974,507 + -0.5 1,974,507 + -0.6 1,974,519 - -0.3 1,974,548 + -0.1 1,974,677 + +2.5 1,974,822 - BPHYT_RS08685 0.40 -0.4 1,974,861 + BPHYT_RS08685 0.57 -0.7 1,974,861 + BPHYT_RS08685 0.57 +0.6 1,974,862 - BPHYT_RS08685 0.58 -0.5 1,974,862 - BPHYT_RS08685 0.58 -1.8 1,974,925 + BPHYT_RS08685 0.86 +2.5 1,974,926 - BPHYT_RS08685 0.86 +1.7
Or see this region's nucleotide sequence