Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS08460 and BPHYT_RS08465 are separated by 81 nucleotides BPHYT_RS08465 and BPHYT_RS08470 are separated by 472 nucleotides
BPHYT_RS08460: BPHYT_RS08460 - 23S rRNA (guanosine-2'-O-)-methyltransferase, at 1,935,640 to 1,936,383
_RS08460
BPHYT_RS08465: BPHYT_RS08465 - ribose-5-phosphate isomerase A, at 1,936,465 to 1,937,160
_RS08465
BPHYT_RS08470: BPHYT_RS08470 - glycosyl transferase, at 1,937,633 to 1,938,829
_RS08470
Position (kb)
1936
1937
1938 Strain fitness (log2 ratio)
-2
-1
0
1 at 1935.492 kb on + strand at 1935.492 kb on + strand at 1935.493 kb on - strand at 1935.493 kb on - strand at 1935.569 kb on + strand at 1935.585 kb on + strand at 1935.585 kb on + strand at 1935.633 kb on - strand at 1935.633 kb on - strand at 1935.662 kb on - strand at 1935.687 kb on + strand at 1936.434 kb on + strand at 1937.271 kb on - strand at 1937.284 kb on - strand at 1937.284 kb on - strand at 1937.584 kb on + strand at 1937.584 kb on + strand at 1937.584 kb on + strand at 1937.585 kb on - strand at 1937.759 kb on - strand, within BPHYT_RS08470 at 1937.834 kb on + strand, within BPHYT_RS08470 at 1937.883 kb on - strand, within BPHYT_RS08470 at 1937.894 kb on - strand, within BPHYT_RS08470 at 1937.897 kb on - strand, within BPHYT_RS08470 at 1937.950 kb on + strand, within BPHYT_RS08470
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 1,935,492 + -0.9 1,935,492 + -0.2 1,935,493 - +0.1 1,935,493 - +0.3 1,935,569 + +0.2 1,935,585 + +0.1 1,935,585 + +1.0 1,935,633 - -1.7 1,935,633 - -0.6 1,935,662 - -0.6 1,935,687 + -0.7 1,936,434 + -0.5 1,937,271 - +0.7 1,937,284 - -2.2 1,937,284 - -0.4 1,937,584 + +0.5 1,937,584 + +0.5 1,937,584 + +1.0 1,937,585 - -0.7 1,937,759 - BPHYT_RS08470 0.11 +0.6 1,937,834 + BPHYT_RS08470 0.17 +0.1 1,937,883 - BPHYT_RS08470 0.21 +0.1 1,937,894 - BPHYT_RS08470 0.22 +0.3 1,937,897 - BPHYT_RS08470 0.22 +0.2 1,937,950 + BPHYT_RS08470 0.26 -1.4
Or see this region's nucleotide sequence