Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS08455

Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS08445 and BPHYT_RS08450 are separated by 241 nucleotidesBPHYT_RS08450 and BPHYT_RS08455 are separated by 351 nucleotidesBPHYT_RS08455 and BPHYT_RS08460 are separated by 185 nucleotides BPHYT_RS08445: BPHYT_RS08445 - tRNA-Leu, at 1,932,187 to 1,932,273 _RS08445 BPHYT_RS08450: BPHYT_RS08450 - tRNA-Leu, at 1,932,515 to 1,932,601 _RS08450 BPHYT_RS08455: BPHYT_RS08455 - ribonuclease R, at 1,932,953 to 1,935,454 _RS08455 BPHYT_RS08460: BPHYT_RS08460 - 23S rRNA (guanosine-2'-O-)-methyltransferase, at 1,935,640 to 1,936,383 _RS08460 Position (kb) 1932 1933 1934 1935 1936Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1932.039 kb on + strandat 1932.313 kb on - strandat 1932.433 kb on - strandat 1932.433 kb on - strandat 1932.433 kb on - strandat 1932.503 kb on + strandat 1932.674 kb on - strandat 1932.725 kb on + strandat 1932.725 kb on + strandat 1932.726 kb on - strandat 1932.760 kb on + strandat 1932.760 kb on + strandat 1932.761 kb on - strandat 1932.775 kb on + strandat 1932.912 kb on - strandat 1933.369 kb on + strand, within BPHYT_RS08455at 1933.369 kb on + strand, within BPHYT_RS08455at 1933.797 kb on - strand, within BPHYT_RS08455at 1933.868 kb on + strand, within BPHYT_RS08455at 1933.869 kb on - strand, within BPHYT_RS08455at 1933.925 kb on + strand, within BPHYT_RS08455at 1933.925 kb on + strand, within BPHYT_RS08455at 1934.058 kb on - strand, within BPHYT_RS08455at 1934.066 kb on + strand, within BPHYT_RS08455at 1934.067 kb on - strand, within BPHYT_RS08455at 1934.103 kb on - strand, within BPHYT_RS08455at 1934.198 kb on + strand, within BPHYT_RS08455at 1934.198 kb on + strand, within BPHYT_RS08455at 1934.198 kb on + strand, within BPHYT_RS08455at 1934.261 kb on + strand, within BPHYT_RS08455at 1934.261 kb on + strand, within BPHYT_RS08455at 1934.261 kb on + strand, within BPHYT_RS08455at 1934.261 kb on + strand, within BPHYT_RS08455at 1934.262 kb on - strand, within BPHYT_RS08455at 1934.637 kb on - strand, within BPHYT_RS08455at 1934.645 kb on + strand, within BPHYT_RS08455at 1934.646 kb on - strand, within BPHYT_RS08455at 1934.729 kb on + strand, within BPHYT_RS08455at 1934.933 kb on + strand, within BPHYT_RS08455at 1934.963 kb on + strand, within BPHYT_RS08455at 1935.065 kb on + strand, within BPHYT_RS08455at 1935.492 kb on + strandat 1935.492 kb on + strandat 1935.493 kb on - strandat 1935.493 kb on - strandat 1935.569 kb on + strandat 1935.585 kb on + strandat 1935.585 kb on + strandat 1935.633 kb on - strandat 1935.633 kb on - strandat 1935.662 kb on - strandat 1935.687 kb on + strandat 1936.434 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
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1,932,039 + +0.5
1,932,313 - -1.0
1,932,433 - -0.5
1,932,433 - -0.2
1,932,433 - -0.6
1,932,503 + +0.1
1,932,674 - +1.8
1,932,725 + -0.5
1,932,725 + +0.1
1,932,726 - +0.8
1,932,760 + +0.3
1,932,760 + -1.2
1,932,761 - +0.3
1,932,775 + -0.3
1,932,912 - -1.7
1,933,369 + BPHYT_RS08455 0.17 -0.9
1,933,369 + BPHYT_RS08455 0.17 -0.5
1,933,797 - BPHYT_RS08455 0.34 -0.7
1,933,868 + BPHYT_RS08455 0.37 -1.4
1,933,869 - BPHYT_RS08455 0.37 +0.7
1,933,925 + BPHYT_RS08455 0.39 +0.4
1,933,925 + BPHYT_RS08455 0.39 -2.6
1,934,058 - BPHYT_RS08455 0.44 +0.3
1,934,066 + BPHYT_RS08455 0.44 -2.9
1,934,067 - BPHYT_RS08455 0.45 -0.1
1,934,103 - BPHYT_RS08455 0.46 +1.0
1,934,198 + BPHYT_RS08455 0.50 -2.8
1,934,198 + BPHYT_RS08455 0.50 -2.3
1,934,198 + BPHYT_RS08455 0.50 +0.0
1,934,261 + BPHYT_RS08455 0.52 -1.5
1,934,261 + BPHYT_RS08455 0.52 -0.3
1,934,261 + BPHYT_RS08455 0.52 -2.7
1,934,261 + BPHYT_RS08455 0.52 -1.8
1,934,262 - BPHYT_RS08455 0.52 +1.1
1,934,637 - BPHYT_RS08455 0.67 -1.0
1,934,645 + BPHYT_RS08455 0.68 -2.0
1,934,646 - BPHYT_RS08455 0.68 +0.5
1,934,729 + BPHYT_RS08455 0.71 -1.4
1,934,933 + BPHYT_RS08455 0.79 -0.3
1,934,963 + BPHYT_RS08455 0.80 -2.2
1,935,065 + BPHYT_RS08455 0.84 -1.5
1,935,492 + -0.9
1,935,492 + -0.2
1,935,493 - +0.1
1,935,493 - +0.3
1,935,569 + +0.2
1,935,585 + +0.1
1,935,585 + +1.0
1,935,633 - -1.7
1,935,633 - -0.6
1,935,662 - -0.6
1,935,687 + -0.7
1,936,434 + -0.5

Or see this region's nucleotide sequence