Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS08075

Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS08070 and BPHYT_RS08075 are separated by 215 nucleotidesBPHYT_RS08075 and BPHYT_RS08080 are separated by 94 nucleotides BPHYT_RS08070: BPHYT_RS08070 - translation initiation factor IF-2, at 1,839,804 to 1,842,764 _RS08070 BPHYT_RS08075: BPHYT_RS08075 - ribosome-binding factor A, at 1,842,980 to 1,843,345 _RS08075 BPHYT_RS08080: BPHYT_RS08080 - tRNA pseudouridine synthase B, at 1,843,440 to 1,844,375 _RS08080 Position (kb) 1842 1843 1844Strain fitness (log2 ratio) -2 -1 0 1at 1842.755 kb on + strandat 1842.755 kb on + strandat 1843.412 kb on - strandat 1843.412 kb on - strandat 1843.421 kb on - strandat 1843.554 kb on + strand, within BPHYT_RS08080at 1843.667 kb on + strand, within BPHYT_RS08080at 1843.751 kb on - strand, within BPHYT_RS08080at 1843.824 kb on + strand, within BPHYT_RS08080at 1843.825 kb on - strand, within BPHYT_RS08080at 1843.857 kb on + strand, within BPHYT_RS08080at 1843.951 kb on + strand, within BPHYT_RS08080at 1843.983 kb on + strand, within BPHYT_RS08080at 1843.983 kb on + strand, within BPHYT_RS08080at 1843.983 kb on + strand, within BPHYT_RS08080at 1843.984 kb on - strand, within BPHYT_RS08080at 1843.984 kb on - strand, within BPHYT_RS08080at 1843.984 kb on - strand, within BPHYT_RS08080at 1843.984 kb on - strand, within BPHYT_RS08080at 1843.984 kb on - strand, within BPHYT_RS08080at 1843.984 kb on - strand, within BPHYT_RS08080

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
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1,842,755 + -1.8
1,842,755 + +0.5
1,843,412 - -0.2
1,843,412 - -0.3
1,843,421 - -2.6
1,843,554 + BPHYT_RS08080 0.12 -0.4
1,843,667 + BPHYT_RS08080 0.24 -0.6
1,843,751 - BPHYT_RS08080 0.33 -0.4
1,843,824 + BPHYT_RS08080 0.41 -1.6
1,843,825 - BPHYT_RS08080 0.41 -1.1
1,843,857 + BPHYT_RS08080 0.45 -0.7
1,843,951 + BPHYT_RS08080 0.55 -0.8
1,843,983 + BPHYT_RS08080 0.58 -0.8
1,843,983 + BPHYT_RS08080 0.58 -1.0
1,843,983 + BPHYT_RS08080 0.58 -1.2
1,843,984 - BPHYT_RS08080 0.58 -1.2
1,843,984 - BPHYT_RS08080 0.58 -0.9
1,843,984 - BPHYT_RS08080 0.58 -0.6
1,843,984 - BPHYT_RS08080 0.58 -0.5
1,843,984 - BPHYT_RS08080 0.58 -0.9
1,843,984 - BPHYT_RS08080 0.58 -0.9

Or see this region's nucleotide sequence