Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS08065 and BPHYT_RS08070 are separated by 93 nucleotides BPHYT_RS08070 and BPHYT_RS08075 are separated by 215 nucleotides BPHYT_RS08075 and BPHYT_RS08080 are separated by 94 nucleotides
BPHYT_RS08065: BPHYT_RS08065 - transcription termination factor NusA, at 1,838,235 to 1,839,710
_RS08065
BPHYT_RS08070: BPHYT_RS08070 - translation initiation factor IF-2, at 1,839,804 to 1,842,764
_RS08070
BPHYT_RS08075: BPHYT_RS08075 - ribosome-binding factor A, at 1,842,980 to 1,843,345
_RS08075
BPHYT_RS08080: BPHYT_RS08080 - tRNA pseudouridine synthase B, at 1,843,440 to 1,844,375
_RS08080
Position (kb)
1839
1840
1841
1842
1843 Strain fitness (log2 ratio)
-2
-1
0
1 at 1839.729 kb on + strand at 1842.755 kb on + strand at 1842.755 kb on + strand at 1843.412 kb on - strand at 1843.412 kb on - strand at 1843.421 kb on - strand at 1843.554 kb on + strand, within BPHYT_RS08080 at 1843.667 kb on + strand, within BPHYT_RS08080 at 1843.751 kb on - strand, within BPHYT_RS08080
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 1,839,729 + -0.2 1,842,755 + -1.8 1,842,755 + +0.5 1,843,412 - -0.2 1,843,412 - -0.3 1,843,421 - -2.6 1,843,554 + BPHYT_RS08080 0.12 -0.4 1,843,667 + BPHYT_RS08080 0.24 -0.6 1,843,751 - BPHYT_RS08080 0.33 -0.4
Or see this region's nucleotide sequence