Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS08050 and BPHYT_RS08055 are separated by 137 nucleotides BPHYT_RS08055 and BPHYT_RS08060 are separated by 157 nucleotides BPHYT_RS08060 and BPHYT_RS08065 overlap by 4 nucleotides
BPHYT_RS08050: BPHYT_RS08050 - pseudouridine synthase, at 1,835,368 to 1,837,248
_RS08050
BPHYT_RS08055: BPHYT_RS08055 - hypothetical protein, at 1,837,386 to 1,837,622
_RS08055
BPHYT_RS08060: BPHYT_RS08060 - ribosome maturation factor RimP, at 1,837,780 to 1,838,238
_RS08060
BPHYT_RS08065: BPHYT_RS08065 - transcription termination factor NusA, at 1,838,235 to 1,839,710
_RS08065
Position (kb)
1837
1838
1839 Strain fitness (log2 ratio)
-1
0
1 at 1837.291 kb on - strand at 1837.370 kb on + strand at 1837.371 kb on - strand at 1837.384 kb on - strand at 1837.768 kb on + strand at 1837.768 kb on + strand at 1837.768 kb on + strand at 1837.869 kb on + strand, within BPHYT_RS08060 at 1837.869 kb on + strand, within BPHYT_RS08060 at 1837.869 kb on + strand, within BPHYT_RS08060 at 1837.869 kb on + strand, within BPHYT_RS08060 at 1837.869 kb on + strand, within BPHYT_RS08060 at 1837.873 kb on + strand, within BPHYT_RS08060 at 1837.873 kb on + strand, within BPHYT_RS08060 at 1837.963 kb on + strand, within BPHYT_RS08060
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 1,837,291 - -0.3 1,837,370 + -1.2 1,837,371 - -0.0 1,837,384 - -0.4 1,837,768 + -0.5 1,837,768 + +1.0 1,837,768 + -0.2 1,837,869 + BPHYT_RS08060 0.19 +0.4 1,837,869 + BPHYT_RS08060 0.19 -0.5 1,837,869 + BPHYT_RS08060 0.19 -0.7 1,837,869 + BPHYT_RS08060 0.19 -1.1 1,837,869 + BPHYT_RS08060 0.19 -1.2 1,837,873 + BPHYT_RS08060 0.20 -0.9 1,837,873 + BPHYT_RS08060 0.20 -0.7 1,837,963 + BPHYT_RS08060 0.40 +0.2
Or see this region's nucleotide sequence