Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS07480 and BPHYT_RS07485 are separated by 113 nucleotides BPHYT_RS07485 and BPHYT_RS07490 are separated by 213 nucleotides BPHYT_RS07490 and BPHYT_RS07495 are separated by 22 nucleotides
BPHYT_RS07480: BPHYT_RS07480 - FAD-dependent oxidoreductase, at 1,700,503 to 1,701,894
_RS07480
BPHYT_RS07485: BPHYT_RS07485 - cupin, at 1,702,008 to 1,702,379
_RS07485
BPHYT_RS07490: BPHYT_RS07490 - aldehyde dehydrogenase, at 1,702,593 to 1,704,029
_RS07490
BPHYT_RS07495: BPHYT_RS07495 - LysR family transcriptional regulator, at 1,704,052 to 1,705,002
_RS07495
Position (kb)
1702
1703
1704
1705 Strain fitness (log2 ratio)
-1
0
1 at 1702.018 kb on + strand at 1702.019 kb on - strand at 1702.253 kb on - strand, within BPHYT_RS07485 at 1702.576 kb on + strand at 1702.578 kb on + strand at 1702.614 kb on + strand at 1702.614 kb on + strand at 1702.615 kb on - strand at 1702.831 kb on - strand, within BPHYT_RS07490 at 1703.326 kb on + strand, within BPHYT_RS07490 at 1703.555 kb on - strand, within BPHYT_RS07490 at 1703.770 kb on - strand, within BPHYT_RS07490 at 1703.895 kb on + strand at 1703.914 kb on - strand at 1704.053 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 1,702,018 + -0.8 1,702,019 - -0.7 1,702,253 - BPHYT_RS07485 0.66 -0.5 1,702,576 + -0.9 1,702,578 + -0.2 1,702,614 + +0.2 1,702,614 + -0.5 1,702,615 - +0.2 1,702,831 - BPHYT_RS07490 0.17 -1.3 1,703,326 + BPHYT_RS07490 0.51 +0.3 1,703,555 - BPHYT_RS07490 0.67 +1.1 1,703,770 - BPHYT_RS07490 0.82 +0.5 1,703,895 + -0.3 1,703,914 - -1.3 1,704,053 + +0.2
Or see this region's nucleotide sequence