Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS06550

Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS06545 and BPHYT_RS06550 are separated by 111 nucleotidesBPHYT_RS06550 and BPHYT_RS06555 are separated by 119 nucleotides BPHYT_RS06545: BPHYT_RS06545 - acetolactate synthase, at 1,485,407 to 1,487,170 _RS06545 BPHYT_RS06550: BPHYT_RS06550 - acetolactate synthase, at 1,487,282 to 1,487,773 _RS06550 BPHYT_RS06555: BPHYT_RS06555 - ketol-acid reductoisomerase, at 1,487,893 to 1,488,909 _RS06555 Position (kb) 1487 1488Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 1486.816 kb on + strand, within BPHYT_RS06545at 1486.844 kb on + strand, within BPHYT_RS06545at 1486.931 kb on + strand, within BPHYT_RS06545at 1486.943 kb on + strand, within BPHYT_RS06545at 1486.943 kb on + strand, within BPHYT_RS06545at 1486.943 kb on + strand, within BPHYT_RS06545at 1486.943 kb on + strand, within BPHYT_RS06545at 1487.166 kb on + strandat 1487.363 kb on + strand, within BPHYT_RS06550at 1487.599 kb on + strand, within BPHYT_RS06550at 1487.701 kb on + strand, within BPHYT_RS06550at 1487.818 kb on + strandat 1487.840 kb on + strandat 1487.840 kb on + strandat 1487.840 kb on + strandat 1487.840 kb on + strandat 1487.840 kb on + strandat 1487.841 kb on - strandat 1487.841 kb on - strandat 1487.841 kb on - strandat 1487.841 kb on - strandat 1487.841 kb on - strandat 1487.842 kb on + strandat 1487.842 kb on + strandat 1487.842 kb on + strandat 1487.842 kb on + strandat 1487.842 kb on + strandat 1487.843 kb on - strandat 1487.843 kb on - strandat 1488.157 kb on + strand, within BPHYT_RS06555at 1488.158 kb on - strand, within BPHYT_RS06555at 1488.229 kb on + strand, within BPHYT_RS06555at 1488.230 kb on - strand, within BPHYT_RS06555at 1488.310 kb on + strand, within BPHYT_RS06555at 1488.544 kb on + strand, within BPHYT_RS06555at 1488.545 kb on - strand, within BPHYT_RS06555at 1488.562 kb on + strand, within BPHYT_RS06555at 1488.562 kb on + strand, within BPHYT_RS06555at 1488.634 kb on + strand, within BPHYT_RS06555at 1488.749 kb on - strand, within BPHYT_RS06555

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
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1,486,816 + BPHYT_RS06545 0.80 -5.2
1,486,844 + BPHYT_RS06545 0.81 -2.8
1,486,931 + BPHYT_RS06545 0.86 -3.3
1,486,943 + BPHYT_RS06545 0.87 -4.8
1,486,943 + BPHYT_RS06545 0.87 -0.2
1,486,943 + BPHYT_RS06545 0.87 -4.6
1,486,943 + BPHYT_RS06545 0.87 -4.0
1,487,166 + +1.6
1,487,363 + BPHYT_RS06550 0.16 -1.4
1,487,599 + BPHYT_RS06550 0.64 -1.7
1,487,701 + BPHYT_RS06550 0.85 -2.2
1,487,818 + -1.3
1,487,840 + -0.1
1,487,840 + -0.7
1,487,840 + -0.8
1,487,840 + +2.1
1,487,840 + -0.8
1,487,841 - -4.1
1,487,841 - -3.0
1,487,841 - -3.0
1,487,841 - -3.2
1,487,841 - -2.4
1,487,842 + +0.3
1,487,842 + -0.5
1,487,842 + -0.2
1,487,842 + -0.7
1,487,842 + +0.6
1,487,843 - -2.8
1,487,843 - -2.9
1,488,157 + BPHYT_RS06555 0.26 -5.7
1,488,158 - BPHYT_RS06555 0.26 +0.1
1,488,229 + BPHYT_RS06555 0.33 -4.5
1,488,230 - BPHYT_RS06555 0.33 -4.8
1,488,310 + BPHYT_RS06555 0.41 -1.8
1,488,544 + BPHYT_RS06555 0.64 -1.3
1,488,545 - BPHYT_RS06555 0.64 -3.7
1,488,562 + BPHYT_RS06555 0.66 -1.1
1,488,562 + BPHYT_RS06555 0.66 -5.6
1,488,634 + BPHYT_RS06555 0.73 -0.8
1,488,749 - BPHYT_RS06555 0.84 -2.7

Or see this region's nucleotide sequence