Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS06530 and BPHYT_RS06535 are separated by 16 nucleotides BPHYT_RS06535 and BPHYT_RS06540 overlap by 4 nucleotides BPHYT_RS06540 and BPHYT_RS06545 are separated by 361 nucleotides
BPHYT_RS06530: BPHYT_RS06530 - membrane protein, at 1,483,282 to 1,484,040
_RS06530
BPHYT_RS06535: BPHYT_RS06535 - membrane protein, at 1,484,057 to 1,484,485
_RS06535
BPHYT_RS06540: BPHYT_RS06540 - RNA polymerase sigma70 factor, at 1,484,482 to 1,485,045
_RS06540
BPHYT_RS06545: BPHYT_RS06545 - acetolactate synthase, at 1,485,407 to 1,487,170
_RS06545
Position (kb) 1484
1485
1486 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2
3
4 at 1483.575 kb on - strand, within BPHYT_RS06530 at 1483.629 kb on - strand, within BPHYT_RS06530 at 1483.967 kb on + strand at 1484.034 kb on - strand at 1484.077 kb on - strand at 1484.101 kb on - strand, within BPHYT_RS06535 at 1484.126 kb on + strand, within BPHYT_RS06535 at 1484.127 kb on - strand, within BPHYT_RS06535 at 1484.127 kb on - strand, within BPHYT_RS06535 at 1484.181 kb on + strand, within BPHYT_RS06535 at 1484.181 kb on + strand, within BPHYT_RS06535 at 1484.485 kb on + strand at 1484.485 kb on + strand at 1484.486 kb on - strand at 1484.601 kb on + strand, within BPHYT_RS06540 at 1484.602 kb on - strand, within BPHYT_RS06540 at 1484.602 kb on - strand, within BPHYT_RS06540 at 1484.811 kb on + strand, within BPHYT_RS06540 at 1484.811 kb on + strand, within BPHYT_RS06540 at 1484.811 kb on + strand, within BPHYT_RS06540 at 1484.811 kb on + strand, within BPHYT_RS06540 at 1484.838 kb on - strand, within BPHYT_RS06540 at 1484.862 kb on + strand, within BPHYT_RS06540 at 1484.862 kb on + strand, within BPHYT_RS06540 at 1484.863 kb on - strand, within BPHYT_RS06540 at 1484.863 kb on - strand, within BPHYT_RS06540 at 1484.975 kb on + strand, within BPHYT_RS06540 at 1485.077 kb on + strand at 1485.113 kb on + strand at 1485.162 kb on + strand at 1485.163 kb on - strand at 1485.163 kb on - strand at 1485.224 kb on - strand at 1485.224 kb on - strand at 1485.253 kb on + strand at 1485.274 kb on + strand at 1485.274 kb on + strand at 1485.275 kb on - strand at 1485.275 kb on - strand at 1485.412 kb on + strand at 1485.551 kb on + strand at 1485.569 kb on + strand at 1485.725 kb on + strand, within BPHYT_RS06545 at 1485.725 kb on + strand, within BPHYT_RS06545 at 1485.725 kb on + strand, within BPHYT_RS06545 at 1485.971 kb on + strand, within BPHYT_RS06545 at 1486.004 kb on + strand, within BPHYT_RS06545 at 1486.004 kb on + strand, within BPHYT_RS06545
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 1,483,575 - BPHYT_RS06530 0.39 -3.7 1,483,629 - BPHYT_RS06530 0.46 -0.2 1,483,967 + -2.2 1,484,034 - -3.6 1,484,077 - -3.7 1,484,101 - BPHYT_RS06535 0.10 +4.2 1,484,126 + BPHYT_RS06535 0.16 -2.6 1,484,127 - BPHYT_RS06535 0.16 -2.0 1,484,127 - BPHYT_RS06535 0.16 -3.2 1,484,181 + BPHYT_RS06535 0.29 -2.9 1,484,181 + BPHYT_RS06535 0.29 -2.4 1,484,485 + -2.2 1,484,485 + -1.6 1,484,486 - -2.6 1,484,601 + BPHYT_RS06540 0.21 -4.3 1,484,602 - BPHYT_RS06540 0.21 -2.0 1,484,602 - BPHYT_RS06540 0.21 +0.4 1,484,811 + BPHYT_RS06540 0.58 -3.7 1,484,811 + BPHYT_RS06540 0.58 -0.9 1,484,811 + BPHYT_RS06540 0.58 -4.8 1,484,811 + BPHYT_RS06540 0.58 -4.0 1,484,838 - BPHYT_RS06540 0.63 -1.8 1,484,862 + BPHYT_RS06540 0.67 -2.2 1,484,862 + BPHYT_RS06540 0.67 -2.0 1,484,863 - BPHYT_RS06540 0.68 -0.7 1,484,863 - BPHYT_RS06540 0.68 -2.1 1,484,975 + BPHYT_RS06540 0.87 -2.0 1,485,077 + -1.1 1,485,113 + -3.3 1,485,162 + -1.3 1,485,163 - -0.1 1,485,163 - +0.8 1,485,224 - +0.1 1,485,224 - -0.7 1,485,253 + -0.7 1,485,274 + -0.3 1,485,274 + -0.9 1,485,275 - -4.2 1,485,275 - -3.1 1,485,412 + -4.8 1,485,551 + -0.2 1,485,569 + -1.4 1,485,725 + BPHYT_RS06545 0.18 +0.1 1,485,725 + BPHYT_RS06545 0.18 -1.4 1,485,725 + BPHYT_RS06545 0.18 -2.6 1,485,971 + BPHYT_RS06545 0.32 -3.6 1,486,004 + BPHYT_RS06545 0.34 -4.5 1,486,004 + BPHYT_RS06545 0.34 -6.2
Or see this region's nucleotide sequence