Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS06400 and BPHYT_RS06405 are separated by 327 nucleotides BPHYT_RS06405 and BPHYT_RS06410 are separated by 247 nucleotides
BPHYT_RS06400: BPHYT_RS06400 - oxygen-dependent coproporphyrinogen-III oxidase, at 1,456,235 to 1,457,158
_RS06400
BPHYT_RS06405: BPHYT_RS06405 - phosphoribosylamine--glycine ligase, at 1,457,486 to 1,458,784
_RS06405
BPHYT_RS06410: BPHYT_RS06410 - transcriptional regulator, at 1,459,032 to 1,459,760
_RS06410
Position (kb)
1457
1458
1459 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 1456.630 kb on - strand, within BPHYT_RS06400 at 1456.674 kb on + strand, within BPHYT_RS06400 at 1456.819 kb on + strand, within BPHYT_RS06400 at 1456.820 kb on - strand, within BPHYT_RS06400 at 1456.820 kb on - strand, within BPHYT_RS06400 at 1456.820 kb on - strand, within BPHYT_RS06400 at 1457.005 kb on - strand, within BPHYT_RS06400 at 1457.260 kb on + strand at 1457.261 kb on - strand at 1457.397 kb on + strand at 1457.398 kb on - strand at 1457.398 kb on - strand at 1457.770 kb on - strand, within BPHYT_RS06405 at 1457.775 kb on + strand, within BPHYT_RS06405 at 1457.775 kb on + strand, within BPHYT_RS06405 at 1457.872 kb on - strand, within BPHYT_RS06405 at 1457.929 kb on + strand, within BPHYT_RS06405 at 1457.930 kb on - strand, within BPHYT_RS06405 at 1458.024 kb on - strand, within BPHYT_RS06405 at 1458.029 kb on - strand, within BPHYT_RS06405 at 1458.390 kb on + strand, within BPHYT_RS06405 at 1458.391 kb on - strand, within BPHYT_RS06405 at 1458.427 kb on - strand, within BPHYT_RS06405 at 1458.699 kb on + strand at 1458.700 kb on - strand at 1458.700 kb on - strand at 1458.776 kb on + strand at 1458.776 kb on + strand at 1458.801 kb on + strand at 1458.810 kb on + strand at 1459.058 kb on + strand at 1459.059 kb on - strand at 1459.493 kb on - strand, within BPHYT_RS06410 at 1459.516 kb on + strand, within BPHYT_RS06410 at 1459.569 kb on - strand, within BPHYT_RS06410 at 1459.725 kb on - strand at 1459.764 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 1,456,630 - BPHYT_RS06400 0.43 -2.8 1,456,674 + BPHYT_RS06400 0.48 -2.9 1,456,819 + BPHYT_RS06400 0.63 -5.1 1,456,820 - BPHYT_RS06400 0.63 -0.8 1,456,820 - BPHYT_RS06400 0.63 -1.3 1,456,820 - BPHYT_RS06400 0.63 -4.8 1,457,005 - BPHYT_RS06400 0.83 +1.4 1,457,260 + -3.3 1,457,261 - +0.1 1,457,397 + -6.1 1,457,398 - -0.3 1,457,398 - +0.4 1,457,770 - BPHYT_RS06405 0.22 -2.0 1,457,775 + BPHYT_RS06405 0.22 -3.3 1,457,775 + BPHYT_RS06405 0.22 -2.3 1,457,872 - BPHYT_RS06405 0.30 -2.0 1,457,929 + BPHYT_RS06405 0.34 -1.5 1,457,930 - BPHYT_RS06405 0.34 -1.1 1,458,024 - BPHYT_RS06405 0.41 -1.4 1,458,029 - BPHYT_RS06405 0.42 +0.1 1,458,390 + BPHYT_RS06405 0.70 -2.3 1,458,391 - BPHYT_RS06405 0.70 +0.4 1,458,427 - BPHYT_RS06405 0.72 -0.4 1,458,699 + -1.7 1,458,700 - -4.1 1,458,700 - -2.6 1,458,776 + +0.4 1,458,776 + -0.2 1,458,801 + -2.8 1,458,810 + +1.4 1,459,058 + -1.1 1,459,059 - -1.5 1,459,493 - BPHYT_RS06410 0.63 +0.2 1,459,516 + BPHYT_RS06410 0.66 -1.7 1,459,569 - BPHYT_RS06410 0.74 -1.7 1,459,725 - -0.0 1,459,764 + -1.8
Or see this region's nucleotide sequence