Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS05115 and BPHYT_RS05120 overlap by 4 nucleotides BPHYT_RS05120 and BPHYT_RS05125 are separated by 12 nucleotides
BPHYT_RS05115: BPHYT_RS05115 - NADH-ubiquinone oxidoreductase subunit 6, at 1,180,155 to 1,181,456
_RS05115
BPHYT_RS05120: BPHYT_RS05120 - hypothetical protein, at 1,181,453 to 1,182,304
_RS05120
BPHYT_RS05125: BPHYT_RS05125 - cyclopropane-fatty-acyl-phospholipid synthase, at 1,182,317 to 1,183,528
_RS05125
Position (kb) 1181
1182
1183 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1180.831 kb on - strand, within BPHYT_RS05115 at 1180.831 kb on - strand, within BPHYT_RS05115 at 1180.841 kb on + strand, within BPHYT_RS05115 at 1180.841 kb on + strand, within BPHYT_RS05115 at 1180.841 kb on + strand, within BPHYT_RS05115 at 1180.842 kb on - strand, within BPHYT_RS05115 at 1180.843 kb on + strand, within BPHYT_RS05115 at 1180.844 kb on - strand, within BPHYT_RS05115 at 1181.040 kb on + strand, within BPHYT_RS05115 at 1181.119 kb on - strand, within BPHYT_RS05115 at 1181.166 kb on + strand, within BPHYT_RS05115 at 1181.182 kb on + strand, within BPHYT_RS05115 at 1181.452 kb on + strand at 1181.555 kb on + strand, within BPHYT_RS05120 at 1181.556 kb on - strand, within BPHYT_RS05120 at 1181.568 kb on - strand, within BPHYT_RS05120 at 1181.710 kb on - strand, within BPHYT_RS05120 at 1181.710 kb on - strand, within BPHYT_RS05120 at 1181.880 kb on - strand, within BPHYT_RS05120 at 1181.880 kb on - strand, within BPHYT_RS05120 at 1181.880 kb on - strand, within BPHYT_RS05120 at 1181.982 kb on - strand, within BPHYT_RS05120 at 1182.032 kb on + strand, within BPHYT_RS05120 at 1182.569 kb on - strand, within BPHYT_RS05125 at 1182.728 kb on + strand, within BPHYT_RS05125 at 1182.728 kb on + strand, within BPHYT_RS05125 at 1182.729 kb on - strand, within BPHYT_RS05125 at 1182.729 kb on - strand, within BPHYT_RS05125 at 1182.750 kb on - strand, within BPHYT_RS05125 at 1182.780 kb on - strand, within BPHYT_RS05125 at 1182.842 kb on + strand, within BPHYT_RS05125 at 1182.842 kb on + strand, within BPHYT_RS05125 at 1182.859 kb on + strand, within BPHYT_RS05125 at 1183.071 kb on - strand, within BPHYT_RS05125
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 1,180,831 - BPHYT_RS05115 0.52 +0.4 1,180,831 - BPHYT_RS05115 0.52 -2.1 1,180,841 + BPHYT_RS05115 0.53 +0.2 1,180,841 + BPHYT_RS05115 0.53 -0.9 1,180,841 + BPHYT_RS05115 0.53 -0.8 1,180,842 - BPHYT_RS05115 0.53 +0.6 1,180,843 + BPHYT_RS05115 0.53 -0.5 1,180,844 - BPHYT_RS05115 0.53 -1.0 1,181,040 + BPHYT_RS05115 0.68 -1.8 1,181,119 - BPHYT_RS05115 0.74 -1.6 1,181,166 + BPHYT_RS05115 0.78 -0.3 1,181,182 + BPHYT_RS05115 0.79 -3.2 1,181,452 + -0.3 1,181,555 + BPHYT_RS05120 0.12 -1.2 1,181,556 - BPHYT_RS05120 0.12 -1.4 1,181,568 - BPHYT_RS05120 0.13 -1.2 1,181,710 - BPHYT_RS05120 0.30 -1.6 1,181,710 - BPHYT_RS05120 0.30 -2.0 1,181,880 - BPHYT_RS05120 0.50 -2.5 1,181,880 - BPHYT_RS05120 0.50 -2.2 1,181,880 - BPHYT_RS05120 0.50 -1.4 1,181,982 - BPHYT_RS05120 0.62 -1.0 1,182,032 + BPHYT_RS05120 0.68 -0.4 1,182,569 - BPHYT_RS05125 0.21 -2.7 1,182,728 + BPHYT_RS05125 0.34 +0.4 1,182,728 + BPHYT_RS05125 0.34 -1.0 1,182,729 - BPHYT_RS05125 0.34 +1.1 1,182,729 - BPHYT_RS05125 0.34 -0.0 1,182,750 - BPHYT_RS05125 0.36 +2.9 1,182,780 - BPHYT_RS05125 0.38 -0.2 1,182,842 + BPHYT_RS05125 0.43 -0.5 1,182,842 + BPHYT_RS05125 0.43 -0.5 1,182,859 + BPHYT_RS05125 0.45 +0.6 1,183,071 - BPHYT_RS05125 0.62 -1.2
Or see this region's nucleotide sequence