Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS05055 and BPHYT_RS05060 are separated by 66 nucleotides BPHYT_RS05060 and BPHYT_RS05065 are separated by 13 nucleotides BPHYT_RS05065 and BPHYT_RS05070 are separated by 180 nucleotides
BPHYT_RS05055: BPHYT_RS05055 - flagellar hook protein FliD, at 1,164,949 to 1,166,457
_RS05055
BPHYT_RS05060: BPHYT_RS05060 - membrane protein, at 1,166,524 to 1,167,144
_RS05060
BPHYT_RS05065: BPHYT_RS05065 - DNA mismatch repair protein MutS, at 1,167,158 to 1,167,916
_RS05065
BPHYT_RS05070: BPHYT_RS05070 - thioredoxin reductase, at 1,168,097 to 1,169,098
_RS05070
Position (kb)
1167
1168 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2
3 at 1166.314 kb on + strand at 1166.398 kb on + strand at 1166.520 kb on - strand at 1166.520 kb on - strand at 1166.603 kb on + strand, within BPHYT_RS05060 at 1166.603 kb on + strand, within BPHYT_RS05060 at 1166.639 kb on + strand, within BPHYT_RS05060 at 1166.640 kb on - strand, within BPHYT_RS05060 at 1166.640 kb on - strand, within BPHYT_RS05060 at 1166.640 kb on - strand, within BPHYT_RS05060 at 1166.640 kb on - strand, within BPHYT_RS05060 at 1166.640 kb on - strand, within BPHYT_RS05060 at 1166.815 kb on + strand, within BPHYT_RS05060 at 1166.815 kb on + strand, within BPHYT_RS05060 at 1166.815 kb on + strand, within BPHYT_RS05060 at 1166.816 kb on - strand, within BPHYT_RS05060 at 1166.816 kb on - strand, within BPHYT_RS05060 at 1166.816 kb on - strand, within BPHYT_RS05060 at 1166.937 kb on - strand, within BPHYT_RS05060 at 1166.958 kb on - strand, within BPHYT_RS05060 at 1167.299 kb on + strand, within BPHYT_RS05065 at 1167.300 kb on - strand, within BPHYT_RS05065 at 1167.734 kb on + strand, within BPHYT_RS05065 at 1167.943 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 1,166,314 + +0.6 1,166,398 + -0.4 1,166,520 - -1.6 1,166,520 - -5.8 1,166,603 + BPHYT_RS05060 0.13 +1.7 1,166,603 + BPHYT_RS05060 0.13 -0.4 1,166,639 + BPHYT_RS05060 0.19 -0.3 1,166,640 - BPHYT_RS05060 0.19 -0.7 1,166,640 - BPHYT_RS05060 0.19 +0.8 1,166,640 - BPHYT_RS05060 0.19 -0.5 1,166,640 - BPHYT_RS05060 0.19 -0.6 1,166,640 - BPHYT_RS05060 0.19 +0.6 1,166,815 + BPHYT_RS05060 0.47 -0.8 1,166,815 + BPHYT_RS05060 0.47 -0.6 1,166,815 + BPHYT_RS05060 0.47 +0.8 1,166,816 - BPHYT_RS05060 0.47 -0.8 1,166,816 - BPHYT_RS05060 0.47 +0.2 1,166,816 - BPHYT_RS05060 0.47 -0.0 1,166,937 - BPHYT_RS05060 0.67 +0.2 1,166,958 - BPHYT_RS05060 0.70 -0.5 1,167,299 + BPHYT_RS05065 0.19 -1.4 1,167,300 - BPHYT_RS05065 0.19 +0.7 1,167,734 + BPHYT_RS05065 0.76 -0.1 1,167,943 - +3.6
Or see this region's nucleotide sequence