Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS04950 and BPHYT_RS04955 are separated by 110 nucleotides BPHYT_RS04955 and BPHYT_RS04960 are separated by 363 nucleotides
BPHYT_RS04950: BPHYT_RS04950 - hypothetical protein, at 1,135,164 to 1,139,495
_RS04950
BPHYT_RS04955: BPHYT_RS04955 - methionyl-tRNA synthetase, at 1,139,606 to 1,141,741
_RS04955
BPHYT_RS04960: BPHYT_RS04960 - membrane protein, at 1,142,105 to 1,142,758
_RS04960
Position (kb)
1139
1140
1141
1142 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 1138.680 kb on - strand, within BPHYT_RS04950 at 1138.935 kb on + strand at 1138.935 kb on + strand, within BPHYT_RS04950 at 1139.015 kb on + strand, within BPHYT_RS04950 at 1139.015 kb on + strand, within BPHYT_RS04950 at 1139.015 kb on + strand, within BPHYT_RS04950 at 1139.016 kb on - strand, within BPHYT_RS04950 at 1139.849 kb on - strand, within BPHYT_RS04955 at 1141.782 kb on + strand at 1141.885 kb on - strand at 1141.888 kb on - strand at 1141.946 kb on + strand at 1142.061 kb on - strand at 1142.259 kb on + strand, within BPHYT_RS04960 at 1142.304 kb on + strand, within BPHYT_RS04960 at 1142.304 kb on + strand, within BPHYT_RS04960 at 1142.323 kb on + strand, within BPHYT_RS04960 at 1142.324 kb on - strand, within BPHYT_RS04960 at 1142.421 kb on + strand, within BPHYT_RS04960 at 1142.421 kb on + strand, within BPHYT_RS04960 at 1142.551 kb on - strand, within BPHYT_RS04960
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 1,138,680 - BPHYT_RS04950 0.81 -0.7 1,138,935 + +3.0 1,138,935 + BPHYT_RS04950 0.87 -0.2 1,139,015 + BPHYT_RS04950 0.89 -0.6 1,139,015 + BPHYT_RS04950 0.89 -1.1 1,139,015 + BPHYT_RS04950 0.89 -0.3 1,139,016 - BPHYT_RS04950 0.89 -0.5 1,139,849 - BPHYT_RS04955 0.11 -5.3 1,141,782 + -0.0 1,141,885 - +0.4 1,141,888 - -0.2 1,141,946 + +0.9 1,142,061 - +0.8 1,142,259 + BPHYT_RS04960 0.24 +0.1 1,142,304 + BPHYT_RS04960 0.30 +0.8 1,142,304 + BPHYT_RS04960 0.30 -0.6 1,142,323 + BPHYT_RS04960 0.33 +0.4 1,142,324 - BPHYT_RS04960 0.33 -0.3 1,142,421 + BPHYT_RS04960 0.48 +0.1 1,142,421 + BPHYT_RS04960 0.48 +0.1 1,142,551 - BPHYT_RS04960 0.68 +0.5
Or see this region's nucleotide sequence