Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS04090 and BPHYT_RS04095 are separated by 560 nucleotides BPHYT_RS04095 and BPHYT_RS04100 are separated by 277 nucleotides BPHYT_RS04100 and BPHYT_RS04105 overlap by 4 nucleotides
BPHYT_RS04090: BPHYT_RS04090 - CBS domain containing membrane protein, at 932,698 to 933,813
_RS04090
BPHYT_RS04095: BPHYT_RS04095 - SAM-dependent methyltransferase PhcB, at 934,374 to 935,192
_RS04095
BPHYT_RS04100: BPHYT_RS04100 - AraC family transcriptional regulator, at 935,470 to 936,000
_RS04100
BPHYT_RS04105: BPHYT_RS04105 - glyoxalase, at 935,997 to 936,413
_RS04105
Position (kb) 934
935
936 Strain fitness (log2 ratio) -2
-1
0
1
2 at 933.383 kb on - strand, within BPHYT_RS04090 at 933.874 kb on - strand at 933.916 kb on - strand at 934.267 kb on - strand at 934.409 kb on + strand at 934.983 kb on + strand, within BPHYT_RS04095 at 935.002 kb on - strand, within BPHYT_RS04095 at 935.285 kb on + strand at 935.285 kb on + strand at 935.369 kb on - strand at 935.433 kb on + strand at 935.724 kb on + strand, within BPHYT_RS04100 at 936.008 kb on + strand at 936.027 kb on + strand at 936.028 kb on - strand at 936.028 kb on - strand at 936.060 kb on + strand, within BPHYT_RS04105
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 933,383 - BPHYT_RS04090 0.61 +0.5 933,874 - +0.5 933,916 - -1.8 934,267 - +1.5 934,409 + +0.2 934,983 + BPHYT_RS04095 0.74 +1.0 935,002 - BPHYT_RS04095 0.77 -1.2 935,285 + +2.0 935,285 + +0.7 935,369 - +2.3 935,433 + +0.1 935,724 + BPHYT_RS04100 0.48 -1.0 936,008 + -1.2 936,027 + -1.4 936,028 - -2.7 936,028 - -0.6 936,060 + BPHYT_RS04105 0.15 +0.2
Or see this region's nucleotide sequence