Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS03725 and BPHYT_RS03730 are separated by 6 nucleotides BPHYT_RS03730 and BPHYT_RS03735 are separated by 198 nucleotides BPHYT_RS03735 and BPHYT_RS03740 overlap by 4 nucleotides BPHYT_RS03740 and BPHYT_RS03745 overlap by 4 nucleotides
BPHYT_RS03725: BPHYT_RS03725 - serine hydroxymethyltransferase, at 845,269 to 846,516
_RS03725
BPHYT_RS03730: BPHYT_RS03730 - transcriptional repressor NrdR, at 846,523 to 847,005
_RS03730
BPHYT_RS03735: BPHYT_RS03735 - pilus assembly protein, at 847,204 to 847,770
_RS03735
BPHYT_RS03740: BPHYT_RS03740 - hypothetical protein, at 847,767 to 848,213
_RS03740
BPHYT_RS03745: BPHYT_RS03745 - cleavage protein, at 848,210 to 849,022
_RS03745
Position (kb) 847
848 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 846.644 kb on - strand, within BPHYT_RS03730 at 846.800 kb on - strand, within BPHYT_RS03730 at 846.893 kb on - strand, within BPHYT_RS03730 at 847.385 kb on - strand at 847.385 kb on - strand, within BPHYT_RS03735 at 847.385 kb on - strand, within BPHYT_RS03735 at 847.579 kb on - strand, within BPHYT_RS03735 at 847.635 kb on + strand, within BPHYT_RS03735 at 847.776 kb on + strand at 847.776 kb on + strand at 847.777 kb on - strand at 847.792 kb on - strand at 848.068 kb on + strand, within BPHYT_RS03740 at 848.068 kb on + strand, within BPHYT_RS03740 at 848.191 kb on + strand at 848.191 kb on + strand at 848.283 kb on + strand at 848.309 kb on + strand, within BPHYT_RS03745 at 848.552 kb on + strand, within BPHYT_RS03745 at 848.675 kb on + strand, within BPHYT_RS03745 at 848.718 kb on - strand, within BPHYT_RS03745
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 846,644 - BPHYT_RS03730 0.25 -3.2 846,800 - BPHYT_RS03730 0.57 -1.2 846,893 - BPHYT_RS03730 0.77 -3.5 847,385 - -0.8 847,385 - BPHYT_RS03735 0.32 +0.7 847,385 - BPHYT_RS03735 0.32 -0.8 847,579 - BPHYT_RS03735 0.66 +0.2 847,635 + BPHYT_RS03735 0.76 -0.1 847,776 + -2.0 847,776 + +0.6 847,777 - -0.8 847,792 - +0.8 848,068 + BPHYT_RS03740 0.67 +0.0 848,068 + BPHYT_RS03740 0.67 +0.3 848,191 + +0.7 848,191 + -0.2 848,283 + +0.2 848,309 + BPHYT_RS03745 0.12 -1.7 848,552 + BPHYT_RS03745 0.42 -0.4 848,675 + BPHYT_RS03745 0.57 -2.0 848,718 - BPHYT_RS03745 0.62 -0.2
Or see this region's nucleotide sequence