Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS03720 and BPHYT_RS03725 are separated by 516 nucleotides BPHYT_RS03725 and BPHYT_RS03730 are separated by 6 nucleotides BPHYT_RS03730 and BPHYT_RS03735 are separated by 198 nucleotides
BPHYT_RS03720: BPHYT_RS03720 - malonic semialdehyde reductase, at 844,006 to 844,752
_RS03720
BPHYT_RS03725: BPHYT_RS03725 - serine hydroxymethyltransferase, at 845,269 to 846,516
_RS03725
BPHYT_RS03730: BPHYT_RS03730 - transcriptional repressor NrdR, at 846,523 to 847,005
_RS03730
BPHYT_RS03735: BPHYT_RS03735 - pilus assembly protein, at 847,204 to 847,770
_RS03735
Position (kb) 845
846
847 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 844.415 kb on - strand, within BPHYT_RS03720 at 844.602 kb on - strand, within BPHYT_RS03720 at 844.602 kb on - strand, within BPHYT_RS03720 at 844.903 kb on + strand at 844.903 kb on + strand at 844.903 kb on + strand at 845.015 kb on + strand at 845.207 kb on + strand at 845.214 kb on + strand at 845.268 kb on + strand at 845.269 kb on - strand at 845.857 kb on + strand, within BPHYT_RS03725 at 846.644 kb on - strand, within BPHYT_RS03730 at 846.800 kb on - strand, within BPHYT_RS03730 at 846.893 kb on - strand, within BPHYT_RS03730 at 847.385 kb on - strand at 847.385 kb on - strand, within BPHYT_RS03735 at 847.385 kb on - strand, within BPHYT_RS03735
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 844,415 - BPHYT_RS03720 0.55 +2.3 844,602 - BPHYT_RS03720 0.80 -0.5 844,602 - BPHYT_RS03720 0.80 -0.6 844,903 + -0.9 844,903 + +1.7 844,903 + +0.7 845,015 + +0.2 845,207 + +1.1 845,214 + +1.1 845,268 + -2.8 845,269 - +1.3 845,857 + BPHYT_RS03725 0.47 +0.1 846,644 - BPHYT_RS03730 0.25 -3.2 846,800 - BPHYT_RS03730 0.57 -1.2 846,893 - BPHYT_RS03730 0.77 -3.5 847,385 - -0.8 847,385 - BPHYT_RS03735 0.32 +0.7 847,385 - BPHYT_RS03735 0.32 -0.8
Or see this region's nucleotide sequence