Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS03710 and BPHYT_RS03715 are separated by 155 nucleotides BPHYT_RS03715 and BPHYT_RS03720 are separated by 70 nucleotides BPHYT_RS03720 and BPHYT_RS03725 are separated by 516 nucleotides
BPHYT_RS03710: BPHYT_RS03710 - protein tolQ, at 842,623 to 843,300
_RS03710
BPHYT_RS03715: BPHYT_RS03715 - 4-hydroxybenzoyl-CoA thioesterase, at 843,456 to 843,935
_RS03715
BPHYT_RS03720: BPHYT_RS03720 - malonic semialdehyde reductase, at 844,006 to 844,752
_RS03720
BPHYT_RS03725: BPHYT_RS03725 - serine hydroxymethyltransferase, at 845,269 to 846,516
_RS03725
Position (kb) 844
845 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 843.426 kb on - strand at 843.675 kb on + strand, within BPHYT_RS03715 at 843.675 kb on + strand, within BPHYT_RS03715 at 843.675 kb on + strand, within BPHYT_RS03715 at 843.676 kb on - strand, within BPHYT_RS03715 at 843.676 kb on - strand, within BPHYT_RS03715 at 843.676 kb on - strand, within BPHYT_RS03715 at 843.676 kb on - strand, within BPHYT_RS03715 at 843.829 kb on + strand, within BPHYT_RS03715 at 843.862 kb on + strand, within BPHYT_RS03715 at 843.865 kb on + strand, within BPHYT_RS03715 at 843.865 kb on + strand, within BPHYT_RS03715 at 843.866 kb on - strand, within BPHYT_RS03715 at 843.887 kb on - strand, within BPHYT_RS03715 at 843.920 kb on + strand at 843.921 kb on - strand at 843.921 kb on - strand at 843.921 kb on - strand at 844.103 kb on + strand, within BPHYT_RS03720 at 844.103 kb on + strand, within BPHYT_RS03720 at 844.104 kb on - strand, within BPHYT_RS03720 at 844.104 kb on - strand, within BPHYT_RS03720 at 844.415 kb on - strand, within BPHYT_RS03720 at 844.602 kb on - strand, within BPHYT_RS03720 at 844.602 kb on - strand, within BPHYT_RS03720 at 844.903 kb on + strand at 844.903 kb on + strand at 844.903 kb on + strand at 845.015 kb on + strand at 845.207 kb on + strand at 845.214 kb on + strand at 845.268 kb on + strand at 845.269 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 843,426 - +0.5 843,675 + BPHYT_RS03715 0.46 +0.6 843,675 + BPHYT_RS03715 0.46 -0.5 843,675 + BPHYT_RS03715 0.46 -0.0 843,676 - BPHYT_RS03715 0.46 +0.3 843,676 - BPHYT_RS03715 0.46 +0.3 843,676 - BPHYT_RS03715 0.46 -1.6 843,676 - BPHYT_RS03715 0.46 +0.3 843,829 + BPHYT_RS03715 0.78 +0.4 843,862 + BPHYT_RS03715 0.85 +5.0 843,865 + BPHYT_RS03715 0.85 +0.6 843,865 + BPHYT_RS03715 0.85 -0.9 843,866 - BPHYT_RS03715 0.85 -0.1 843,887 - BPHYT_RS03715 0.90 +0.2 843,920 + +2.1 843,921 - +0.1 843,921 - -0.4 843,921 - +1.5 844,103 + BPHYT_RS03720 0.13 -1.6 844,103 + BPHYT_RS03720 0.13 +0.2 844,104 - BPHYT_RS03720 0.13 +0.3 844,104 - BPHYT_RS03720 0.13 +1.9 844,415 - BPHYT_RS03720 0.55 +2.3 844,602 - BPHYT_RS03720 0.80 -0.5 844,602 - BPHYT_RS03720 0.80 -0.6 844,903 + -0.9 844,903 + +1.7 844,903 + +0.7 845,015 + +0.2 845,207 + +1.1 845,214 + +1.1 845,268 + -2.8 845,269 - +1.3
Or see this region's nucleotide sequence