Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS03655 and BPHYT_RS03660 are separated by 131 nucleotides BPHYT_RS03660 and BPHYT_RS03665 are separated by 233 nucleotides
BPHYT_RS03655: BPHYT_RS03655 - membrane protein, at 831,305 to 831,976
_RS03655
BPHYT_RS03660: BPHYT_RS03660 - mechanosensitive ion channel protein MscS, at 832,108 to 832,920
_RS03660
BPHYT_RS03665: BPHYT_RS03665 - hypothetical protein, at 833,154 to 833,519
_RS03665
Position (kb) 832
833 Strain fitness (log2 ratio) -2
-1
0
1
2 at 831.109 kb on + strand at 831.110 kb on - strand at 831.152 kb on + strand at 831.192 kb on + strand at 831.193 kb on - strand at 831.293 kb on - strand at 831.318 kb on + strand at 831.567 kb on - strand, within BPHYT_RS03655 at 831.833 kb on + strand, within BPHYT_RS03655 at 831.983 kb on - strand at 832.211 kb on + strand, within BPHYT_RS03660 at 832.416 kb on - strand, within BPHYT_RS03660 at 832.416 kb on - strand, within BPHYT_RS03660 at 832.416 kb on - strand, within BPHYT_RS03660 at 832.416 kb on - strand, within BPHYT_RS03660 at 832.426 kb on - strand, within BPHYT_RS03660 at 832.745 kb on + strand, within BPHYT_RS03660 at 832.974 kb on + strand at 833.040 kb on - strand at 833.324 kb on - strand, within BPHYT_RS03665 at 833.349 kb on + strand, within BPHYT_RS03665 at 833.350 kb on - strand, within BPHYT_RS03665 at 833.350 kb on - strand, within BPHYT_RS03665 at 833.405 kb on + strand, within BPHYT_RS03665 at 833.406 kb on - strand, within BPHYT_RS03665 at 833.519 kb on + strand at 833.519 kb on + strand at 833.520 kb on - strand at 833.520 kb on - strand at 833.624 kb on - strand at 833.757 kb on + strand at 833.758 kb on - strand at 833.775 kb on - strand at 833.812 kb on - strand at 833.812 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 831,109 + +0.1 831,110 - -0.3 831,152 + +1.7 831,192 + +0.4 831,193 - +0.1 831,293 - -1.7 831,318 + -0.5 831,567 - BPHYT_RS03655 0.39 -1.7 831,833 + BPHYT_RS03655 0.79 -0.7 831,983 - -1.4 832,211 + BPHYT_RS03660 0.13 +1.0 832,416 - BPHYT_RS03660 0.38 -1.2 832,416 - BPHYT_RS03660 0.38 -0.4 832,416 - BPHYT_RS03660 0.38 +1.1 832,416 - BPHYT_RS03660 0.38 +0.8 832,426 - BPHYT_RS03660 0.39 +0.5 832,745 + BPHYT_RS03660 0.78 -2.2 832,974 + +0.0 833,040 - -0.7 833,324 - BPHYT_RS03665 0.46 +1.0 833,349 + BPHYT_RS03665 0.53 +0.0 833,350 - BPHYT_RS03665 0.54 -2.1 833,350 - BPHYT_RS03665 0.54 +1.3 833,405 + BPHYT_RS03665 0.69 +0.2 833,406 - BPHYT_RS03665 0.69 +1.9 833,519 + -0.8 833,519 + -0.4 833,520 - +0.7 833,520 - +0.0 833,624 - +0.6 833,757 + -0.1 833,758 - +1.7 833,775 - -0.5 833,812 - -0.4 833,812 - -0.4
Or see this region's nucleotide sequence