Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS03150 and BPHYT_RS03155 are separated by 34 nucleotides BPHYT_RS03155 and BPHYT_RS03160 are separated by 84 nucleotides BPHYT_RS03160 and BPHYT_RS03165 are separated by 67 nucleotides
BPHYT_RS03150: BPHYT_RS03150 - Phosphoglycerate dehydrogenase (EC:1.1.1.95) <small>(from data)</small>, at 704,985 to 709,079
_RS03150
BPHYT_RS03155: BPHYT_RS03155 - histidine triad (HIT) protein, at 709,114 to 709,542
_RS03155
BPHYT_RS03160: BPHYT_RS03160 - hypothetical protein, at 709,627 to 710,040
_RS03160
BPHYT_RS03165: BPHYT_RS03165 - ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE, at 710,108 to 710,839
_RS03165
Position (kb) 709
710 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 708.181 kb on - strand, within BPHYT_RS03150 at 708.181 kb on - strand, within BPHYT_RS03150 at 708.282 kb on + strand, within BPHYT_RS03150 at 708.282 kb on + strand, within BPHYT_RS03150 at 708.297 kb on + strand, within BPHYT_RS03150 at 708.549 kb on + strand, within BPHYT_RS03150 at 708.640 kb on - strand, within BPHYT_RS03150 at 708.949 kb on - strand at 708.949 kb on - strand at 708.978 kb on + strand at 708.979 kb on - strand at 709.032 kb on + strand at 709.032 kb on + strand at 709.294 kb on + strand, within BPHYT_RS03155 at 709.491 kb on + strand at 709.492 kb on - strand, within BPHYT_RS03155 at 709.492 kb on - strand, within BPHYT_RS03155 at 709.492 kb on - strand, within BPHYT_RS03155 at 709.616 kb on - strand at 709.626 kb on + strand at 709.626 kb on + strand at 709.705 kb on + strand, within BPHYT_RS03160 at 709.706 kb on - strand, within BPHYT_RS03160 at 709.754 kb on - strand, within BPHYT_RS03160 at 709.754 kb on - strand, within BPHYT_RS03160 at 709.823 kb on + strand, within BPHYT_RS03160 at 709.856 kb on - strand, within BPHYT_RS03160 at 709.900 kb on + strand, within BPHYT_RS03160 at 709.900 kb on + strand, within BPHYT_RS03160 at 709.900 kb on + strand, within BPHYT_RS03160 at 710.041 kb on + strand at 710.041 kb on + strand at 710.088 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 708,181 - BPHYT_RS03150 0.78 -5.1 708,181 - BPHYT_RS03150 0.78 -1.6 708,282 + BPHYT_RS03150 0.81 -4.2 708,282 + BPHYT_RS03150 0.81 -4.2 708,297 + BPHYT_RS03150 0.81 -3.7 708,549 + BPHYT_RS03150 0.87 -3.1 708,640 - BPHYT_RS03150 0.89 -5.8 708,949 - -5.2 708,949 - -2.8 708,978 + -2.0 708,979 - -3.7 709,032 + -5.3 709,032 + -3.4 709,294 + BPHYT_RS03155 0.42 -0.6 709,491 + +1.8 709,492 - BPHYT_RS03155 0.88 -1.2 709,492 - BPHYT_RS03155 0.88 -0.6 709,492 - BPHYT_RS03155 0.88 -0.2 709,616 - -0.6 709,626 + -1.1 709,626 + +0.0 709,705 + BPHYT_RS03160 0.19 +1.9 709,706 - BPHYT_RS03160 0.19 -0.9 709,754 - BPHYT_RS03160 0.31 +0.1 709,754 - BPHYT_RS03160 0.31 -0.9 709,823 + BPHYT_RS03160 0.47 -0.0 709,856 - BPHYT_RS03160 0.55 -1.2 709,900 + BPHYT_RS03160 0.66 -0.0 709,900 + BPHYT_RS03160 0.66 -0.6 709,900 + BPHYT_RS03160 0.66 -0.0 710,041 + +1.5 710,041 + +0.8 710,088 + -0.7
Or see this region's nucleotide sequence