Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS03145 and BPHYT_RS03150 are separated by 504 nucleotides BPHYT_RS03150 and BPHYT_RS03155 are separated by 34 nucleotides BPHYT_RS03155 and BPHYT_RS03160 are separated by 84 nucleotides
BPHYT_RS03145: BPHYT_RS03145 - threonine dehydratase, at 702,957 to 704,480
_RS03145
BPHYT_RS03150: BPHYT_RS03150 - Phosphoglycerate dehydrogenase (EC:1.1.1.95) <small>(from data)</small>, at 704,985 to 709,079
_RS03150
BPHYT_RS03155: BPHYT_RS03155 - histidine triad (HIT) protein, at 709,114 to 709,542
_RS03155
BPHYT_RS03160: BPHYT_RS03160 - hypothetical protein, at 709,627 to 710,040
_RS03160
Position (kb) 704
705
706
707
708
709
710 Strain fitness (log2 ratio) -8
-7
-6
-5
-4
-3
-2
-1
0
1
2 at 704.539 kb on + strand at 704.540 kb on - strand at 704.708 kb on - strand at 704.830 kb on + strand at 704.835 kb on - strand at 704.835 kb on - strand at 704.835 kb on - strand at 704.835 kb on - strand at 704.836 kb on + strand at 704.837 kb on - strand at 704.837 kb on - strand at 704.895 kb on + strand at 704.968 kb on + strand at 704.968 kb on + strand at 705.075 kb on + strand at 705.076 kb on - strand at 705.675 kb on - strand, within BPHYT_RS03150 at 706.089 kb on + strand, within BPHYT_RS03150 at 706.089 kb on + strand, within BPHYT_RS03150 at 706.090 kb on - strand, within BPHYT_RS03150 at 706.090 kb on - strand, within BPHYT_RS03150 at 706.275 kb on - strand, within BPHYT_RS03150 at 706.348 kb on - strand, within BPHYT_RS03150 at 706.950 kb on + strand, within BPHYT_RS03150 at 706.950 kb on + strand, within BPHYT_RS03150 at 706.951 kb on - strand, within BPHYT_RS03150 at 706.951 kb on - strand, within BPHYT_RS03150 at 706.951 kb on - strand, within BPHYT_RS03150 at 707.026 kb on + strand, within BPHYT_RS03150 at 707.026 kb on + strand, within BPHYT_RS03150 at 707.027 kb on - strand, within BPHYT_RS03150 at 707.027 kb on - strand, within BPHYT_RS03150 at 707.245 kb on - strand, within BPHYT_RS03150 at 707.391 kb on + strand, within BPHYT_RS03150 at 707.392 kb on - strand, within BPHYT_RS03150 at 707.392 kb on - strand, within BPHYT_RS03150 at 707.494 kb on - strand, within BPHYT_RS03150 at 707.494 kb on - strand, within BPHYT_RS03150 at 707.617 kb on - strand, within BPHYT_RS03150 at 707.628 kb on - strand, within BPHYT_RS03150 at 707.813 kb on + strand, within BPHYT_RS03150 at 708.181 kb on - strand, within BPHYT_RS03150 at 708.181 kb on - strand, within BPHYT_RS03150 at 708.282 kb on + strand, within BPHYT_RS03150 at 708.282 kb on + strand, within BPHYT_RS03150 at 708.297 kb on + strand, within BPHYT_RS03150 at 708.549 kb on + strand, within BPHYT_RS03150 at 708.640 kb on - strand, within BPHYT_RS03150 at 708.949 kb on - strand at 708.949 kb on - strand at 708.978 kb on + strand at 708.979 kb on - strand at 709.032 kb on + strand at 709.032 kb on + strand at 709.294 kb on + strand, within BPHYT_RS03155 at 709.491 kb on + strand at 709.492 kb on - strand, within BPHYT_RS03155 at 709.492 kb on - strand, within BPHYT_RS03155 at 709.492 kb on - strand, within BPHYT_RS03155 at 709.616 kb on - strand at 709.626 kb on + strand at 709.626 kb on + strand at 709.705 kb on + strand, within BPHYT_RS03160 at 709.706 kb on - strand, within BPHYT_RS03160 at 709.754 kb on - strand, within BPHYT_RS03160 at 709.754 kb on - strand, within BPHYT_RS03160 at 709.823 kb on + strand, within BPHYT_RS03160 at 709.856 kb on - strand, within BPHYT_RS03160 at 709.900 kb on + strand, within BPHYT_RS03160 at 709.900 kb on + strand, within BPHYT_RS03160 at 709.900 kb on + strand, within BPHYT_RS03160 at 710.041 kb on + strand at 710.041 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 704,539 + +0.5 704,540 - +0.3 704,708 - -2.6 704,830 + -1.8 704,835 - -6.5 704,835 - -3.5 704,835 - -1.3 704,835 - -5.8 704,836 + -0.0 704,837 - -1.4 704,837 - -3.8 704,895 + +1.6 704,968 + +0.8 704,968 + +0.7 705,075 + -7.4 705,076 - -2.6 705,675 - BPHYT_RS03150 0.17 -2.8 706,089 + BPHYT_RS03150 0.27 -4.3 706,089 + BPHYT_RS03150 0.27 +0.3 706,090 - BPHYT_RS03150 0.27 -6.2 706,090 - BPHYT_RS03150 0.27 -3.6 706,275 - BPHYT_RS03150 0.32 -5.4 706,348 - BPHYT_RS03150 0.33 -3.9 706,950 + BPHYT_RS03150 0.48 -4.8 706,950 + BPHYT_RS03150 0.48 -0.5 706,951 - BPHYT_RS03150 0.48 -3.0 706,951 - BPHYT_RS03150 0.48 -4.1 706,951 - BPHYT_RS03150 0.48 -8.2 707,026 + BPHYT_RS03150 0.50 -3.6 707,026 + BPHYT_RS03150 0.50 -1.3 707,027 - BPHYT_RS03150 0.50 -1.6 707,027 - BPHYT_RS03150 0.50 -3.0 707,245 - BPHYT_RS03150 0.55 -4.7 707,391 + BPHYT_RS03150 0.59 -2.8 707,392 - BPHYT_RS03150 0.59 -4.0 707,392 - BPHYT_RS03150 0.59 -3.5 707,494 - BPHYT_RS03150 0.61 -4.7 707,494 - BPHYT_RS03150 0.61 -5.8 707,617 - BPHYT_RS03150 0.64 -2.4 707,628 - BPHYT_RS03150 0.65 -6.3 707,813 + BPHYT_RS03150 0.69 -4.8 708,181 - BPHYT_RS03150 0.78 -5.1 708,181 - BPHYT_RS03150 0.78 -1.6 708,282 + BPHYT_RS03150 0.81 -4.2 708,282 + BPHYT_RS03150 0.81 -4.2 708,297 + BPHYT_RS03150 0.81 -3.7 708,549 + BPHYT_RS03150 0.87 -3.1 708,640 - BPHYT_RS03150 0.89 -5.8 708,949 - -5.2 708,949 - -2.8 708,978 + -2.0 708,979 - -3.7 709,032 + -5.3 709,032 + -3.4 709,294 + BPHYT_RS03155 0.42 -0.6 709,491 + +1.8 709,492 - BPHYT_RS03155 0.88 -1.2 709,492 - BPHYT_RS03155 0.88 -0.6 709,492 - BPHYT_RS03155 0.88 -0.2 709,616 - -0.6 709,626 + -1.1 709,626 + +0.0 709,705 + BPHYT_RS03160 0.19 +1.9 709,706 - BPHYT_RS03160 0.19 -0.9 709,754 - BPHYT_RS03160 0.31 +0.1 709,754 - BPHYT_RS03160 0.31 -0.9 709,823 + BPHYT_RS03160 0.47 -0.0 709,856 - BPHYT_RS03160 0.55 -1.2 709,900 + BPHYT_RS03160 0.66 -0.0 709,900 + BPHYT_RS03160 0.66 -0.6 709,900 + BPHYT_RS03160 0.66 -0.0 710,041 + +1.5 710,041 + +0.8
Or see this region's nucleotide sequence