Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS03135 and BPHYT_RS03140 overlap by 4 nucleotides BPHYT_RS03140 and BPHYT_RS03145 are separated by 23 nucleotides BPHYT_RS03145 and BPHYT_RS03150 are separated by 504 nucleotides
BPHYT_RS03135: BPHYT_RS03135 - NADPH-dependent 7-cyano-7-deazaguanine reductase, at 701,210 to 702,034
_RS03135
BPHYT_RS03140: BPHYT_RS03140 - 5'-nucleotidase, at 702,031 to 702,933
_RS03140
BPHYT_RS03145: BPHYT_RS03145 - threonine dehydratase, at 702,957 to 704,480
_RS03145
BPHYT_RS03150: BPHYT_RS03150 - Phosphoglycerate dehydrogenase (EC:1.1.1.95) <small>(from data)</small>, at 704,985 to 709,079
_RS03150
Position (kb) 702
703
704
705 Strain fitness (log2 ratio) -7
-6
-5
-4
-3
-2
-1
0
1 at 701.982 kb on + strand at 701.983 kb on - strand at 702.265 kb on - strand, within BPHYT_RS03140 at 702.270 kb on + strand, within BPHYT_RS03140 at 702.270 kb on + strand, within BPHYT_RS03140 at 702.270 kb on + strand, within BPHYT_RS03140 at 702.271 kb on - strand, within BPHYT_RS03140 at 702.271 kb on - strand, within BPHYT_RS03140 at 702.271 kb on - strand, within BPHYT_RS03140 at 702.631 kb on + strand, within BPHYT_RS03140 at 702.632 kb on - strand, within BPHYT_RS03140 at 702.828 kb on - strand, within BPHYT_RS03140 at 702.851 kb on + strand at 702.954 kb on + strand at 702.954 kb on + strand at 702.955 kb on - strand at 702.955 kb on - strand at 704.539 kb on + strand at 704.540 kb on - strand at 704.708 kb on - strand at 704.830 kb on + strand at 704.835 kb on - strand at 704.835 kb on - strand at 704.835 kb on - strand at 704.835 kb on - strand at 704.836 kb on + strand at 704.837 kb on - strand at 704.837 kb on - strand at 704.895 kb on + strand at 704.968 kb on + strand at 704.968 kb on + strand at 705.075 kb on + strand at 705.076 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 701,982 + -4.0 701,983 - -2.7 702,265 - BPHYT_RS03140 0.26 +0.2 702,270 + BPHYT_RS03140 0.26 -0.1 702,270 + BPHYT_RS03140 0.26 +0.7 702,270 + BPHYT_RS03140 0.26 +0.4 702,271 - BPHYT_RS03140 0.27 +0.5 702,271 - BPHYT_RS03140 0.27 -0.2 702,271 - BPHYT_RS03140 0.27 -0.8 702,631 + BPHYT_RS03140 0.66 -0.8 702,632 - BPHYT_RS03140 0.67 -0.7 702,828 - BPHYT_RS03140 0.88 -0.6 702,851 + +0.3 702,954 + +0.2 702,954 + -0.6 702,955 - -0.7 702,955 - -0.8 704,539 + +0.5 704,540 - +0.3 704,708 - -2.6 704,830 + -1.8 704,835 - -6.5 704,835 - -3.5 704,835 - -1.3 704,835 - -5.8 704,836 + -0.0 704,837 - -1.4 704,837 - -3.8 704,895 + +1.6 704,968 + +0.8 704,968 + +0.7 705,075 + -7.4 705,076 - -2.6
Or see this region's nucleotide sequence