Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS03130 and BPHYT_RS03135 are separated by 74 nucleotides BPHYT_RS03135 and BPHYT_RS03140 overlap by 4 nucleotides BPHYT_RS03140 and BPHYT_RS03145 are separated by 23 nucleotides
BPHYT_RS03130: BPHYT_RS03130 - hypothetical protein, at 700,749 to 701,135
_RS03130
BPHYT_RS03135: BPHYT_RS03135 - NADPH-dependent 7-cyano-7-deazaguanine reductase, at 701,210 to 702,034
_RS03135
BPHYT_RS03140: BPHYT_RS03140 - 5'-nucleotidase, at 702,031 to 702,933
_RS03140
BPHYT_RS03145: BPHYT_RS03145 - threonine dehydratase, at 702,957 to 704,480
_RS03145
Position (kb) 702
703 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 701.031 kb on + strand, within BPHYT_RS03130 at 701.115 kb on + strand at 701.116 kb on - strand at 701.117 kb on + strand at 701.117 kb on + strand at 701.118 kb on - strand at 701.212 kb on - strand at 701.212 kb on - strand at 701.253 kb on + strand at 701.254 kb on - strand at 701.297 kb on - strand, within BPHYT_RS03135 at 701.298 kb on + strand, within BPHYT_RS03135 at 701.307 kb on + strand, within BPHYT_RS03135 at 701.307 kb on + strand, within BPHYT_RS03135 at 701.364 kb on - strand, within BPHYT_RS03135 at 701.580 kb on + strand, within BPHYT_RS03135 at 701.581 kb on - strand, within BPHYT_RS03135 at 701.581 kb on - strand, within BPHYT_RS03135 at 701.581 kb on - strand, within BPHYT_RS03135 at 701.581 kb on - strand, within BPHYT_RS03135 at 701.772 kb on + strand, within BPHYT_RS03135 at 701.880 kb on + strand, within BPHYT_RS03135 at 701.880 kb on + strand, within BPHYT_RS03135 at 701.881 kb on - strand, within BPHYT_RS03135 at 701.881 kb on - strand, within BPHYT_RS03135 at 701.982 kb on + strand at 701.983 kb on - strand at 702.265 kb on - strand, within BPHYT_RS03140 at 702.270 kb on + strand, within BPHYT_RS03140 at 702.270 kb on + strand, within BPHYT_RS03140 at 702.270 kb on + strand, within BPHYT_RS03140 at 702.271 kb on - strand, within BPHYT_RS03140 at 702.271 kb on - strand, within BPHYT_RS03140 at 702.271 kb on - strand, within BPHYT_RS03140 at 702.631 kb on + strand, within BPHYT_RS03140 at 702.632 kb on - strand, within BPHYT_RS03140 at 702.828 kb on - strand, within BPHYT_RS03140 at 702.851 kb on + strand at 702.954 kb on + strand at 702.954 kb on + strand at 702.955 kb on - strand at 702.955 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 701,031 + BPHYT_RS03130 0.73 +1.0 701,115 + -0.0 701,116 - +0.2 701,117 + +1.0 701,117 + -1.6 701,118 - -0.8 701,212 - +0.5 701,212 - +1.6 701,253 + -2.7 701,254 - -3.2 701,297 - BPHYT_RS03135 0.11 -1.0 701,298 + BPHYT_RS03135 0.11 -3.6 701,307 + BPHYT_RS03135 0.12 -3.3 701,307 + BPHYT_RS03135 0.12 -2.5 701,364 - BPHYT_RS03135 0.19 -3.4 701,580 + BPHYT_RS03135 0.45 -5.0 701,581 - BPHYT_RS03135 0.45 -6.1 701,581 - BPHYT_RS03135 0.45 -0.5 701,581 - BPHYT_RS03135 0.45 -2.7 701,581 - BPHYT_RS03135 0.45 -1.4 701,772 + BPHYT_RS03135 0.68 -3.4 701,880 + BPHYT_RS03135 0.81 -1.6 701,880 + BPHYT_RS03135 0.81 -3.0 701,881 - BPHYT_RS03135 0.81 -1.0 701,881 - BPHYT_RS03135 0.81 -0.5 701,982 + -4.0 701,983 - -2.7 702,265 - BPHYT_RS03140 0.26 +0.2 702,270 + BPHYT_RS03140 0.26 -0.1 702,270 + BPHYT_RS03140 0.26 +0.7 702,270 + BPHYT_RS03140 0.26 +0.4 702,271 - BPHYT_RS03140 0.27 +0.5 702,271 - BPHYT_RS03140 0.27 -0.2 702,271 - BPHYT_RS03140 0.27 -0.8 702,631 + BPHYT_RS03140 0.66 -0.8 702,632 - BPHYT_RS03140 0.67 -0.7 702,828 - BPHYT_RS03140 0.88 -0.6 702,851 + +0.3 702,954 + +0.2 702,954 + -0.6 702,955 - -0.7 702,955 - -0.8
Or see this region's nucleotide sequence