Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS03035 and BPHYT_RS03040 overlap by 14 nucleotides BPHYT_RS03040 and BPHYT_RS03045 are separated by 200 nucleotides BPHYT_RS03045 and BPHYT_RS03050 overlap by 4 nucleotides
BPHYT_RS03035: BPHYT_RS03035 - cytochrome C oxidase subunit I, at 676,241 to 677,866
_RS03035
BPHYT_RS03040: BPHYT_RS03040 - cytochrome C oxidase subunit II, at 677,853 to 678,425
_RS03040
BPHYT_RS03045: BPHYT_RS03045 - alcohol dehydrogenase, at 678,626 to 680,011
_RS03045
BPHYT_RS03050: BPHYT_RS03050 - cytochrome C, at 680,008 to 680,721
_RS03050
Position (kb) 678
679
680
681 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 677.692 kb on - strand, within BPHYT_RS03035 at 677.692 kb on - strand, within BPHYT_RS03035 at 677.692 kb on - strand, within BPHYT_RS03035 at 678.465 kb on + strand at 678.466 kb on - strand at 678.604 kb on - strand at 678.604 kb on - strand at 678.708 kb on + strand at 678.708 kb on + strand at 678.708 kb on + strand at 678.709 kb on - strand at 678.709 kb on - strand at 678.709 kb on - strand at 678.709 kb on - strand at 678.709 kb on - strand at 678.709 kb on - strand at 678.768 kb on + strand, within BPHYT_RS03045 at 678.967 kb on + strand, within BPHYT_RS03045 at 678.967 kb on + strand, within BPHYT_RS03045 at 678.968 kb on - strand, within BPHYT_RS03045 at 678.968 kb on - strand, within BPHYT_RS03045 at 678.968 kb on - strand, within BPHYT_RS03045 at 678.971 kb on - strand, within BPHYT_RS03045 at 679.062 kb on + strand, within BPHYT_RS03045 at 679.062 kb on + strand, within BPHYT_RS03045 at 679.062 kb on + strand, within BPHYT_RS03045 at 679.063 kb on - strand, within BPHYT_RS03045 at 679.077 kb on + strand, within BPHYT_RS03045 at 679.077 kb on + strand, within BPHYT_RS03045 at 679.078 kb on - strand, within BPHYT_RS03045 at 679.101 kb on + strand, within BPHYT_RS03045 at 679.270 kb on + strand, within BPHYT_RS03045 at 679.271 kb on - strand, within BPHYT_RS03045 at 679.320 kb on + strand, within BPHYT_RS03045 at 679.384 kb on - strand, within BPHYT_RS03045 at 679.401 kb on + strand, within BPHYT_RS03045 at 679.402 kb on - strand, within BPHYT_RS03045 at 679.524 kb on + strand, within BPHYT_RS03045 at 679.524 kb on + strand, within BPHYT_RS03045 at 679.524 kb on + strand, within BPHYT_RS03045 at 679.525 kb on - strand, within BPHYT_RS03045 at 679.525 kb on - strand, within BPHYT_RS03045 at 679.656 kb on + strand, within BPHYT_RS03045 at 679.810 kb on + strand, within BPHYT_RS03045 at 679.810 kb on + strand, within BPHYT_RS03045 at 679.811 kb on - strand, within BPHYT_RS03045 at 680.216 kb on + strand, within BPHYT_RS03050 at 680.216 kb on + strand, within BPHYT_RS03050 at 680.432 kb on + strand, within BPHYT_RS03050 at 680.459 kb on + strand, within BPHYT_RS03050 at 680.460 kb on - strand, within BPHYT_RS03050 at 680.475 kb on - strand, within BPHYT_RS03050 at 680.519 kb on + strand, within BPHYT_RS03050 at 680.519 kb on + strand, within BPHYT_RS03050 at 680.552 kb on + strand, within BPHYT_RS03050 at 680.552 kb on + strand, within BPHYT_RS03050 at 680.553 kb on - strand, within BPHYT_RS03050 at 680.671 kb on + strand at 680.671 kb on + strand at 680.678 kb on + strand at 680.679 kb on - strand at 680.706 kb on + strand at 680.706 kb on + strand at 680.747 kb on + strand at 680.748 kb on - strand at 680.760 kb on - strand at 680.810 kb on + strand at 680.810 kb on + strand at 680.810 kb on + strand at 680.839 kb on + strand at 680.841 kb on + strand at 680.842 kb on - strand at 680.921 kb on + strand at 680.921 kb on + strand at 680.922 kb on - strand at 680.923 kb on + strand at 681.007 kb on + strand at 681.007 kb on + strand at 681.008 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 677,692 - BPHYT_RS03035 0.89 +0.1 677,692 - BPHYT_RS03035 0.89 +0.2 677,692 - BPHYT_RS03035 0.89 +0.3 678,465 + +0.9 678,466 - +1.2 678,604 - +0.8 678,604 - -0.5 678,708 + -1.0 678,708 + +0.2 678,708 + +1.0 678,709 - +0.6 678,709 - +1.3 678,709 - -0.4 678,709 - +0.3 678,709 - -0.3 678,709 - -0.8 678,768 + BPHYT_RS03045 0.10 -0.6 678,967 + BPHYT_RS03045 0.25 -0.1 678,967 + BPHYT_RS03045 0.25 +1.2 678,968 - BPHYT_RS03045 0.25 +0.0 678,968 - BPHYT_RS03045 0.25 +0.6 678,968 - BPHYT_RS03045 0.25 -2.3 678,971 - BPHYT_RS03045 0.25 -0.2 679,062 + BPHYT_RS03045 0.31 -0.3 679,062 + BPHYT_RS03045 0.31 +0.2 679,062 + BPHYT_RS03045 0.31 -1.6 679,063 - BPHYT_RS03045 0.32 +0.5 679,077 + BPHYT_RS03045 0.33 -0.3 679,077 + BPHYT_RS03045 0.33 -1.3 679,078 - BPHYT_RS03045 0.33 -0.4 679,101 + BPHYT_RS03045 0.34 -0.2 679,270 + BPHYT_RS03045 0.46 +0.0 679,271 - BPHYT_RS03045 0.47 +0.3 679,320 + BPHYT_RS03045 0.50 -1.0 679,384 - BPHYT_RS03045 0.55 -2.1 679,401 + BPHYT_RS03045 0.56 +0.2 679,402 - BPHYT_RS03045 0.56 -2.2 679,524 + BPHYT_RS03045 0.65 +1.8 679,524 + BPHYT_RS03045 0.65 +0.5 679,524 + BPHYT_RS03045 0.65 +0.9 679,525 - BPHYT_RS03045 0.65 -0.1 679,525 - BPHYT_RS03045 0.65 -0.2 679,656 + BPHYT_RS03045 0.74 -0.6 679,810 + BPHYT_RS03045 0.85 +0.3 679,810 + BPHYT_RS03045 0.85 +0.0 679,811 - BPHYT_RS03045 0.85 -0.0 680,216 + BPHYT_RS03050 0.29 -2.8 680,216 + BPHYT_RS03050 0.29 +0.1 680,432 + BPHYT_RS03050 0.59 +0.8 680,459 + BPHYT_RS03050 0.63 +1.3 680,460 - BPHYT_RS03050 0.63 +0.0 680,475 - BPHYT_RS03050 0.65 -0.8 680,519 + BPHYT_RS03050 0.72 +0.3 680,519 + BPHYT_RS03050 0.72 -0.1 680,552 + BPHYT_RS03050 0.76 +1.6 680,552 + BPHYT_RS03050 0.76 -1.5 680,553 - BPHYT_RS03050 0.76 -0.2 680,671 + -0.1 680,671 + +0.6 680,678 + -0.6 680,679 - +0.6 680,706 + +0.6 680,706 + +3.7 680,747 + +1.0 680,748 - +1.8 680,760 - +0.2 680,810 + +0.1 680,810 + -0.5 680,810 + +0.0 680,839 + -0.5 680,841 + +2.3 680,842 - +0.3 680,921 + -0.9 680,921 + +0.8 680,922 - +0.7 680,923 + -0.4 681,007 + -0.9 681,007 + -0.0 681,008 - +1.1
Or see this region's nucleotide sequence