Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02695 and BPHYT_RS02700 are separated by 67 nucleotides BPHYT_RS02700 and BPHYT_RS02705 are separated by 265 nucleotides BPHYT_RS02705 and BPHYT_RS02710 are separated by 52 nucleotides BPHYT_RS02710 and BPHYT_RS02715 are separated by 11 nucleotides
BPHYT_RS02695: BPHYT_RS02695 - photosynthetic protein synthase I, at 605,154 to 605,771
_RS02695
BPHYT_RS02700: BPHYT_RS02700 - hypothetical protein, at 605,839 to 606,129
_RS02700
BPHYT_RS02705: BPHYT_RS02705 - membrane protein, at 606,395 to 608,269
_RS02705
BPHYT_RS02710: BPHYT_RS02710 - hypothetical protein, at 608,322 to 608,507
_RS02710
BPHYT_RS02715: BPHYT_RS02715 - dioxygenase, at 608,519 to 609,331
_RS02715
Position (kb)
606
607
608
609 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 605.526 kb on + strand, within BPHYT_RS02695 at 605.527 kb on - strand, within BPHYT_RS02695 at 605.527 kb on - strand, within BPHYT_RS02695 at 605.650 kb on - strand, within BPHYT_RS02695 at 605.650 kb on - strand, within BPHYT_RS02695 at 605.676 kb on + strand, within BPHYT_RS02695 at 605.676 kb on + strand, within BPHYT_RS02695 at 605.677 kb on - strand, within BPHYT_RS02695 at 605.677 kb on - strand, within BPHYT_RS02695 at 606.107 kb on + strand at 606.107 kb on + strand at 606.108 kb on - strand at 606.111 kb on + strand at 606.112 kb on - strand at 606.125 kb on + strand at 606.125 kb on + strand at 606.126 kb on - strand at 606.126 kb on - strand at 606.298 kb on - strand at 606.298 kb on - strand at 606.298 kb on - strand at 606.305 kb on + strand at 606.328 kb on - strand at 606.414 kb on + strand at 606.415 kb on - strand at 606.415 kb on - strand at 606.415 kb on - strand at 606.416 kb on - strand at 606.761 kb on + strand, within BPHYT_RS02705 at 606.860 kb on + strand, within BPHYT_RS02705 at 606.860 kb on + strand, within BPHYT_RS02705 at 606.860 kb on + strand, within BPHYT_RS02705 at 606.861 kb on - strand, within BPHYT_RS02705 at 606.861 kb on - strand, within BPHYT_RS02705 at 606.861 kb on - strand, within BPHYT_RS02705 at 606.935 kb on + strand, within BPHYT_RS02705 at 606.936 kb on - strand, within BPHYT_RS02705 at 607.370 kb on - strand, within BPHYT_RS02705 at 608.333 kb on - strand at 608.339 kb on - strand at 608.352 kb on - strand, within BPHYT_RS02710 at 608.499 kb on + strand at 608.717 kb on + strand, within BPHYT_RS02715 at 608.718 kb on - strand, within BPHYT_RS02715 at 608.783 kb on + strand, within BPHYT_RS02715 at 608.784 kb on - strand, within BPHYT_RS02715 at 608.813 kb on + strand, within BPHYT_RS02715 at 608.813 kb on + strand, within BPHYT_RS02715 at 608.840 kb on + strand, within BPHYT_RS02715 at 608.840 kb on + strand, within BPHYT_RS02715 at 608.840 kb on + strand, within BPHYT_RS02715 at 608.840 kb on + strand, within BPHYT_RS02715 at 608.840 kb on + strand, within BPHYT_RS02715 at 608.841 kb on - strand, within BPHYT_RS02715 at 608.841 kb on - strand, within BPHYT_RS02715 at 608.841 kb on - strand, within BPHYT_RS02715 at 608.841 kb on - strand, within BPHYT_RS02715 at 608.841 kb on - strand, within BPHYT_RS02715 at 608.861 kb on + strand, within BPHYT_RS02715 at 609.011 kb on - strand, within BPHYT_RS02715 at 609.187 kb on + strand, within BPHYT_RS02715 at 609.226 kb on + strand, within BPHYT_RS02715 at 609.227 kb on - strand, within BPHYT_RS02715 at 609.264 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 605,526 + BPHYT_RS02695 0.60 -2.6 605,527 - BPHYT_RS02695 0.60 -3.6 605,527 - BPHYT_RS02695 0.60 -1.9 605,650 - BPHYT_RS02695 0.80 -1.1 605,650 - BPHYT_RS02695 0.80 +0.4 605,676 + BPHYT_RS02695 0.84 -1.7 605,676 + BPHYT_RS02695 0.84 -0.5 605,677 - BPHYT_RS02695 0.85 -0.5 605,677 - BPHYT_RS02695 0.85 -2.0 606,107 + +0.7 606,107 + +1.2 606,108 - -6.4 606,111 + +0.6 606,112 - -0.3 606,125 + +0.9 606,125 + +0.7 606,126 - +1.0 606,126 - +0.1 606,298 - -1.0 606,298 - +0.6 606,298 - -2.3 606,305 + +0.2 606,328 - +0.7 606,414 + +0.2 606,415 - -0.6 606,415 - +1.0 606,415 - +0.3 606,416 - -3.1 606,761 + BPHYT_RS02705 0.20 -0.3 606,860 + BPHYT_RS02705 0.25 -0.5 606,860 + BPHYT_RS02705 0.25 -0.8 606,860 + BPHYT_RS02705 0.25 -0.8 606,861 - BPHYT_RS02705 0.25 +2.6 606,861 - BPHYT_RS02705 0.25 +0.3 606,861 - BPHYT_RS02705 0.25 -0.7 606,935 + BPHYT_RS02705 0.29 -0.5 606,936 - BPHYT_RS02705 0.29 -0.4 607,370 - BPHYT_RS02705 0.52 +0.5 608,333 - -0.1 608,339 - -0.4 608,352 - BPHYT_RS02710 0.16 +0.1 608,499 + +0.2 608,717 + BPHYT_RS02715 0.24 -0.3 608,718 - BPHYT_RS02715 0.24 +0.3 608,783 + BPHYT_RS02715 0.32 +1.3 608,784 - BPHYT_RS02715 0.33 -0.8 608,813 + BPHYT_RS02715 0.36 +2.3 608,813 + BPHYT_RS02715 0.36 -0.0 608,840 + BPHYT_RS02715 0.39 +1.5 608,840 + BPHYT_RS02715 0.39 +0.5 608,840 + BPHYT_RS02715 0.39 +0.1 608,840 + BPHYT_RS02715 0.39 -0.3 608,840 + BPHYT_RS02715 0.39 +1.0 608,841 - BPHYT_RS02715 0.40 -2.7 608,841 - BPHYT_RS02715 0.40 -0.1 608,841 - BPHYT_RS02715 0.40 +0.2 608,841 - BPHYT_RS02715 0.40 -1.1 608,841 - BPHYT_RS02715 0.40 -0.1 608,861 + BPHYT_RS02715 0.42 +0.3 609,011 - BPHYT_RS02715 0.61 +0.3 609,187 + BPHYT_RS02715 0.82 +0.5 609,226 + BPHYT_RS02715 0.87 -0.7 609,227 - BPHYT_RS02715 0.87 -1.4 609,264 - -0.2
Or see this region's nucleotide sequence