Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS02665

Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS02655 and BPHYT_RS02660 are separated by 36 nucleotidesBPHYT_RS02660 and BPHYT_RS02665 are separated by 132 nucleotidesBPHYT_RS02665 and BPHYT_RS02670 are separated by 181 nucleotidesBPHYT_RS02670 and BPHYT_RS02675 are separated by 111 nucleotides BPHYT_RS02655: BPHYT_RS02655 - cytochrome C oxidase assembly protein, at 599,282 to 599,920 _RS02655 BPHYT_RS02660: BPHYT_RS02660 - membrane protein, at 599,957 to 600,166 _RS02660 BPHYT_RS02665: BPHYT_RS02665 - MFS transporter, at 600,299 to 601,156 _RS02665 BPHYT_RS02670: BPHYT_RS02670 - membrane protein, at 601,338 to 601,547 _RS02670 BPHYT_RS02675: BPHYT_RS02675 - hypothetical protein, at 601,659 to 602,375 _RS02675 Position (kb) 600 601 602Strain fitness (log2 ratio) -7 -6 -5 -4 -3 -2 -1 0 1at 599.450 kb on - strand, within BPHYT_RS02655at 599.470 kb on + strand, within BPHYT_RS02655at 599.594 kb on + strand, within BPHYT_RS02655at 599.594 kb on + strand, within BPHYT_RS02655at 599.649 kb on - strand, within BPHYT_RS02655at 599.673 kb on - strand, within BPHYT_RS02655at 599.755 kb on - strand, within BPHYT_RS02655at 600.124 kb on + strand, within BPHYT_RS02660at 600.156 kb on + strandat 600.159 kb on + strandat 600.184 kb on + strandat 600.251 kb on + strandat 600.252 kb on - strandat 600.323 kb on + strandat 600.323 kb on + strandat 600.326 kb on + strandat 600.466 kb on + strand, within BPHYT_RS02665at 600.518 kb on + strand, within BPHYT_RS02665at 600.518 kb on + strand, within BPHYT_RS02665at 600.519 kb on - strand, within BPHYT_RS02665at 600.519 kb on - strand, within BPHYT_RS02665at 600.519 kb on - strand, within BPHYT_RS02665at 600.614 kb on + strand, within BPHYT_RS02665at 600.614 kb on + strand, within BPHYT_RS02665at 600.614 kb on + strand, within BPHYT_RS02665at 600.615 kb on - strand, within BPHYT_RS02665at 600.615 kb on - strand, within BPHYT_RS02665at 600.656 kb on + strand, within BPHYT_RS02665at 600.657 kb on - strand, within BPHYT_RS02665at 600.918 kb on - strand, within BPHYT_RS02665at 600.918 kb on - strand, within BPHYT_RS02665at 600.953 kb on + strand, within BPHYT_RS02665at 600.954 kb on - strand, within BPHYT_RS02665at 600.954 kb on - strand, within BPHYT_RS02665at 600.954 kb on - strandat 601.039 kb on + strand, within BPHYT_RS02665at 601.057 kb on + strandat 601.058 kb on - strandat 601.134 kb on - strandat 601.134 kb on - strandat 601.134 kb on - strandat 601.259 kb on + strandat 601.259 kb on + strandat 601.268 kb on - strandat 601.349 kb on - strandat 601.356 kb on - strandat 601.588 kb on - strandat 601.890 kb on + strand, within BPHYT_RS02675at 601.890 kb on + strand, within BPHYT_RS02675at 601.908 kb on + strand, within BPHYT_RS02675at 601.908 kb on + strand, within BPHYT_RS02675at 601.909 kb on - strand, within BPHYT_RS02675at 601.929 kb on + strand, within BPHYT_RS02675at 601.930 kb on - strand, within BPHYT_RS02675at 601.965 kb on + strand, within BPHYT_RS02675at 601.966 kb on - strand, within BPHYT_RS02675at 601.966 kb on - strand, within BPHYT_RS02675at 602.001 kb on - strand, within BPHYT_RS02675at 602.001 kb on - strand, within BPHYT_RS02675at 602.029 kb on - strand, within BPHYT_RS02675at 602.092 kb on - strand, within BPHYT_RS02675

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
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599,450 - BPHYT_RS02655 0.26 -3.0
599,470 + BPHYT_RS02655 0.29 +0.7
599,594 + BPHYT_RS02655 0.49 +0.2
599,594 + BPHYT_RS02655 0.49 -0.0
599,649 - BPHYT_RS02655 0.57 -1.3
599,673 - BPHYT_RS02655 0.61 -3.4
599,755 - BPHYT_RS02655 0.74 -3.7
600,124 + BPHYT_RS02660 0.80 -1.3
600,156 + -0.4
600,159 + +0.1
600,184 + +0.5
600,251 + +0.5
600,252 - +0.3
600,323 + -3.7
600,323 + -1.3
600,326 + -1.7
600,466 + BPHYT_RS02665 0.19 -5.0
600,518 + BPHYT_RS02665 0.26 -6.3
600,518 + BPHYT_RS02665 0.26 -4.0
600,519 - BPHYT_RS02665 0.26 -3.2
600,519 - BPHYT_RS02665 0.26 -4.3
600,519 - BPHYT_RS02665 0.26 -5.5
600,614 + BPHYT_RS02665 0.37 -6.3
600,614 + BPHYT_RS02665 0.37 -4.8
600,614 + BPHYT_RS02665 0.37 -6.3
600,615 - BPHYT_RS02665 0.37 -2.6
600,615 - BPHYT_RS02665 0.37 -2.1
600,656 + BPHYT_RS02665 0.42 -1.5
600,657 - BPHYT_RS02665 0.42 -6.2
600,918 - BPHYT_RS02665 0.72 -1.8
600,918 - BPHYT_RS02665 0.72 -4.1
600,953 + BPHYT_RS02665 0.76 -6.9
600,954 - BPHYT_RS02665 0.76 -6.5
600,954 - BPHYT_RS02665 0.76 -6.2
600,954 - -1.3
601,039 + BPHYT_RS02665 0.86 -4.7
601,057 + -0.9
601,058 - +1.3
601,134 - -2.6
601,134 - -2.2
601,134 - -3.5
601,259 + -0.3
601,259 + -2.0
601,268 - -0.6
601,349 - -1.8
601,356 - -2.4
601,588 - -2.5
601,890 + BPHYT_RS02675 0.32 -2.0
601,890 + BPHYT_RS02675 0.32 -0.1
601,908 + BPHYT_RS02675 0.35 -1.0
601,908 + BPHYT_RS02675 0.35 -4.2
601,909 - BPHYT_RS02675 0.35 -3.5
601,929 + BPHYT_RS02675 0.38 -1.5
601,930 - BPHYT_RS02675 0.38 -3.4
601,965 + BPHYT_RS02675 0.43 -1.0
601,966 - BPHYT_RS02675 0.43 -1.5
601,966 - BPHYT_RS02675 0.43 -0.9
602,001 - BPHYT_RS02675 0.48 -4.2
602,001 - BPHYT_RS02675 0.48 -1.8
602,029 - BPHYT_RS02675 0.52 -0.5
602,092 - BPHYT_RS02675 0.60 -3.0

Or see this region's nucleotide sequence