Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02555 and BPHYT_RS02560 are separated by 101 nucleotides BPHYT_RS02560 and BPHYT_RS02565 are separated by 337 nucleotides BPHYT_RS02565 and BPHYT_RS02570 are separated by 96 nucleotides
BPHYT_RS02555: BPHYT_RS02555 - glutamate--cysteine ligase, at 580,181 to 581,470
_RS02555
BPHYT_RS02560: BPHYT_RS02560 - glutathione synthetase, at 581,572 to 582,528
_RS02560
BPHYT_RS02565: BPHYT_RS02565 - PTS mannose transporter subunit IIA, at 582,866 to 583,393
_RS02565
BPHYT_RS02570: BPHYT_RS02570 - phosphocarrier protein HPr, at 583,490 to 583,759
_RS02570
Position (kb) 581
582
583 Strain fitness (log2 ratio) -7
-6
-5
-4
-3
-2
-1
0
1
2
3
4 at 580.757 kb on + strand, within BPHYT_RS02555 at 580.757 kb on + strand, within BPHYT_RS02555 at 580.758 kb on - strand at 580.758 kb on - strand, within BPHYT_RS02555 at 580.792 kb on - strand, within BPHYT_RS02555 at 580.820 kb on + strand, within BPHYT_RS02555 at 580.883 kb on + strand, within BPHYT_RS02555 at 580.884 kb on - strand, within BPHYT_RS02555 at 580.884 kb on - strand, within BPHYT_RS02555 at 580.983 kb on - strand, within BPHYT_RS02555 at 581.012 kb on + strand, within BPHYT_RS02555 at 581.012 kb on + strand, within BPHYT_RS02555 at 581.012 kb on + strand, within BPHYT_RS02555 at 581.013 kb on - strand, within BPHYT_RS02555 at 581.013 kb on - strand, within BPHYT_RS02555 at 581.043 kb on - strand, within BPHYT_RS02555 at 581.171 kb on + strand, within BPHYT_RS02555 at 581.171 kb on + strand, within BPHYT_RS02555 at 581.172 kb on - strand, within BPHYT_RS02555 at 581.216 kb on + strand, within BPHYT_RS02555 at 581.216 kb on + strand, within BPHYT_RS02555 at 581.216 kb on + strand, within BPHYT_RS02555 at 581.231 kb on + strand, within BPHYT_RS02555 at 581.384 kb on + strand at 581.385 kb on - strand at 581.385 kb on - strand at 581.385 kb on - strand at 581.386 kb on + strand at 581.386 kb on + strand at 581.386 kb on + strand at 581.387 kb on - strand at 581.387 kb on - strand at 581.387 kb on - strand at 581.387 kb on - strand at 581.390 kb on + strand at 581.390 kb on + strand at 581.390 kb on + strand at 581.390 kb on + strand at 581.390 kb on + strand at 581.391 kb on - strand at 581.391 kb on - strand at 581.468 kb on + strand at 581.469 kb on - strand at 581.525 kb on + strand at 581.527 kb on + strand at 581.527 kb on + strand at 581.527 kb on + strand at 581.527 kb on + strand at 581.527 kb on + strand at 581.528 kb on - strand at 581.528 kb on - strand at 581.532 kb on - strand at 581.617 kb on + strand at 581.632 kb on + strand at 581.674 kb on + strand, within BPHYT_RS02560 at 581.674 kb on + strand, within BPHYT_RS02560 at 581.675 kb on - strand, within BPHYT_RS02560 at 581.675 kb on - strand, within BPHYT_RS02560 at 581.675 kb on - strand, within BPHYT_RS02560 at 581.739 kb on + strand, within BPHYT_RS02560 at 581.739 kb on + strand, within BPHYT_RS02560 at 581.740 kb on - strand, within BPHYT_RS02560 at 581.740 kb on - strand, within BPHYT_RS02560 at 581.866 kb on + strand, within BPHYT_RS02560 at 581.867 kb on - strand, within BPHYT_RS02560 at 581.867 kb on - strand, within BPHYT_RS02560 at 581.867 kb on - strand, within BPHYT_RS02560 at 581.867 kb on - strand, within BPHYT_RS02560 at 581.867 kb on - strand, within BPHYT_RS02560 at 582.584 kb on + strand at 582.584 kb on + strand at 582.585 kb on - strand at 582.633 kb on + strand at 582.634 kb on - strand at 582.634 kb on - strand at 582.634 kb on - strand at 582.634 kb on - strand at 582.701 kb on + strand at 583.389 kb on + strand at 583.391 kb on + strand at 583.391 kb on + strand at 583.391 kb on + strand at 583.392 kb on - strand at 583.392 kb on - strand at 583.449 kb on + strand at 583.468 kb on + strand at 583.511 kb on - strand at 583.511 kb on - strand at 583.511 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 580,757 + BPHYT_RS02555 0.45 -5.3 580,757 + BPHYT_RS02555 0.45 -3.5 580,758 - -2.0 580,758 - BPHYT_RS02555 0.45 -3.8 580,792 - BPHYT_RS02555 0.47 -1.3 580,820 + BPHYT_RS02555 0.50 -3.7 580,883 + BPHYT_RS02555 0.54 -2.7 580,884 - BPHYT_RS02555 0.54 -2.9 580,884 - BPHYT_RS02555 0.54 -1.7 580,983 - BPHYT_RS02555 0.62 -3.8 581,012 + BPHYT_RS02555 0.64 -6.7 581,012 + BPHYT_RS02555 0.64 -2.5 581,012 + BPHYT_RS02555 0.64 -3.7 581,013 - BPHYT_RS02555 0.64 -6.9 581,013 - BPHYT_RS02555 0.64 -4.5 581,043 - BPHYT_RS02555 0.67 -6.5 581,171 + BPHYT_RS02555 0.77 -4.1 581,171 + BPHYT_RS02555 0.77 -4.8 581,172 - BPHYT_RS02555 0.77 -1.8 581,216 + BPHYT_RS02555 0.80 -0.5 581,216 + BPHYT_RS02555 0.80 -1.0 581,216 + BPHYT_RS02555 0.80 -4.4 581,231 + BPHYT_RS02555 0.81 -3.7 581,384 + -2.7 581,385 - -4.4 581,385 - -4.8 581,385 - -6.2 581,386 + -4.6 581,386 + -4.2 581,386 + -4.3 581,387 - -2.1 581,387 - -6.2 581,387 - -4.2 581,387 - -1.7 581,390 + -4.7 581,390 + -4.0 581,390 + -2.7 581,390 + -2.3 581,390 + +0.3 581,391 - -5.3 581,391 - -4.7 581,468 + +0.3 581,469 - -0.3 581,525 + -1.6 581,527 + -1.6 581,527 + -0.0 581,527 + -0.4 581,527 + -0.2 581,527 + -0.5 581,528 - -2.3 581,528 - -1.1 581,532 - -0.6 581,617 + -3.1 581,632 + -3.1 581,674 + BPHYT_RS02560 0.11 -1.4 581,674 + BPHYT_RS02560 0.11 +0.3 581,675 - BPHYT_RS02560 0.11 -4.8 581,675 - BPHYT_RS02560 0.11 -2.7 581,675 - BPHYT_RS02560 0.11 +0.0 581,739 + BPHYT_RS02560 0.17 -3.5 581,739 + BPHYT_RS02560 0.17 -2.7 581,740 - BPHYT_RS02560 0.18 -3.9 581,740 - BPHYT_RS02560 0.18 -1.3 581,866 + BPHYT_RS02560 0.31 -2.6 581,867 - BPHYT_RS02560 0.31 -2.3 581,867 - BPHYT_RS02560 0.31 -1.0 581,867 - BPHYT_RS02560 0.31 -0.0 581,867 - BPHYT_RS02560 0.31 -2.7 581,867 - BPHYT_RS02560 0.31 -3.1 582,584 + +0.1 582,584 + +2.3 582,585 - +0.4 582,633 + -2.3 582,634 - -0.5 582,634 - +1.9 582,634 - +1.0 582,634 - -0.9 582,701 + +4.3 583,389 + +2.3 583,391 + +0.2 583,391 + -1.7 583,391 + -0.9 583,392 - -0.3 583,392 - -0.6 583,449 + -0.0 583,468 + -0.1 583,511 - -4.2 583,511 - -5.8 583,511 - -5.4
Or see this region's nucleotide sequence