Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02520 and BPHYT_RS02525 are separated by 78 nucleotides BPHYT_RS02525 and BPHYT_RS02530 are separated by 173 nucleotides
BPHYT_RS02520: BPHYT_RS02520 - Fis family transcriptional regulator, at 572,474 to 573,823
_RS02520
BPHYT_RS02525: BPHYT_RS02525 - membrane protein, at 573,902 to 574,432
_RS02525
BPHYT_RS02530: BPHYT_RS02530 - hypothetical protein, at 574,606 to 575,319
_RS02530
Position (kb) 573
574
575 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2
3 at 573.143 kb on - strand, within BPHYT_RS02520 at 573.246 kb on + strand, within BPHYT_RS02520 at 573.341 kb on - strand, within BPHYT_RS02520 at 573.770 kb on + strand at 573.963 kb on - strand, within BPHYT_RS02525 at 573.963 kb on - strand, within BPHYT_RS02525 at 573.966 kb on + strand, within BPHYT_RS02525 at 573.966 kb on + strand, within BPHYT_RS02525 at 574.095 kb on + strand, within BPHYT_RS02525 at 574.096 kb on - strand, within BPHYT_RS02525 at 574.096 kb on - strand, within BPHYT_RS02525 at 574.107 kb on + strand at 574.107 kb on + strand, within BPHYT_RS02525 at 574.107 kb on + strand, within BPHYT_RS02525 at 574.108 kb on - strand, within BPHYT_RS02525 at 574.108 kb on - strand, within BPHYT_RS02525 at 574.108 kb on - strand, within BPHYT_RS02525 at 574.123 kb on - strand, within BPHYT_RS02525 at 574.171 kb on + strand, within BPHYT_RS02525 at 574.173 kb on + strand, within BPHYT_RS02525 at 574.173 kb on + strand, within BPHYT_RS02525 at 574.174 kb on - strand, within BPHYT_RS02525 at 574.174 kb on - strand, within BPHYT_RS02525 at 574.174 kb on - strand, within BPHYT_RS02525 at 574.243 kb on - strand, within BPHYT_RS02525 at 574.243 kb on - strand, within BPHYT_RS02525 at 574.338 kb on + strand, within BPHYT_RS02525 at 574.339 kb on - strand, within BPHYT_RS02525 at 574.383 kb on + strand at 574.383 kb on + strand at 574.384 kb on - strand at 574.514 kb on + strand at 574.519 kb on + strand at 574.564 kb on - strand at 574.566 kb on + strand at 574.567 kb on - strand at 574.575 kb on + strand at 574.575 kb on + strand at 574.575 kb on + strand at 574.576 kb on - strand at 574.576 kb on - strand at 574.576 kb on - strand at 574.889 kb on - strand, within BPHYT_RS02530 at 575.100 kb on + strand, within BPHYT_RS02530 at 575.207 kb on - strand, within BPHYT_RS02530 at 575.239 kb on + strand, within BPHYT_RS02530 at 575.239 kb on + strand, within BPHYT_RS02530 at 575.314 kb on + strand at 575.406 kb on + strand at 575.418 kb on + strand at 575.418 kb on + strand at 575.419 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 573,143 - BPHYT_RS02520 0.50 +1.4 573,246 + BPHYT_RS02520 0.57 -0.0 573,341 - BPHYT_RS02520 0.64 -1.1 573,770 + -1.8 573,963 - BPHYT_RS02525 0.11 -2.3 573,963 - BPHYT_RS02525 0.11 -1.2 573,966 + BPHYT_RS02525 0.12 -0.7 573,966 + BPHYT_RS02525 0.12 -1.4 574,095 + BPHYT_RS02525 0.36 -1.2 574,096 - BPHYT_RS02525 0.37 -3.2 574,096 - BPHYT_RS02525 0.37 -4.2 574,107 + +0.7 574,107 + BPHYT_RS02525 0.39 -2.2 574,107 + BPHYT_RS02525 0.39 -1.3 574,108 - BPHYT_RS02525 0.39 -3.9 574,108 - BPHYT_RS02525 0.39 -3.1 574,108 - BPHYT_RS02525 0.39 -1.4 574,123 - BPHYT_RS02525 0.42 -2.0 574,171 + BPHYT_RS02525 0.51 -0.5 574,173 + BPHYT_RS02525 0.51 -1.7 574,173 + BPHYT_RS02525 0.51 -3.3 574,174 - BPHYT_RS02525 0.51 +0.7 574,174 - BPHYT_RS02525 0.51 -3.1 574,174 - BPHYT_RS02525 0.51 -1.8 574,243 - BPHYT_RS02525 0.64 -4.2 574,243 - BPHYT_RS02525 0.64 -1.2 574,338 + BPHYT_RS02525 0.82 -1.6 574,339 - BPHYT_RS02525 0.82 -3.8 574,383 + -0.9 574,383 + -1.2 574,384 - -3.2 574,514 + -6.4 574,519 + -2.7 574,564 - -0.1 574,566 + +0.3 574,567 - -0.3 574,575 + -0.6 574,575 + -0.8 574,575 + +0.2 574,576 - -0.3 574,576 - -1.7 574,576 - +0.3 574,889 - BPHYT_RS02530 0.40 -1.3 575,100 + BPHYT_RS02530 0.69 -0.4 575,207 - BPHYT_RS02530 0.84 -0.5 575,239 + BPHYT_RS02530 0.89 -0.4 575,239 + BPHYT_RS02530 0.89 -0.4 575,314 + -0.5 575,406 + +3.2 575,418 + +1.2 575,418 + +0.3 575,419 - +0.5
Or see this region's nucleotide sequence