Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS02465

Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS02455 and BPHYT_RS02460 are separated by 41 nucleotidesBPHYT_RS02460 and BPHYT_RS02465 overlap by 4 nucleotidesBPHYT_RS02465 and BPHYT_RS02470 are separated by 27 nucleotidesBPHYT_RS02470 and BPHYT_RS02475 are separated by 218 nucleotides BPHYT_RS02455: BPHYT_RS02455 - taurine ABC transporter substrate-binding protein, at 557,015 to 557,998 _RS02455 BPHYT_RS02460: BPHYT_RS02460 - nitrate ABC transporter ATP-binding protein, at 558,040 to 558,837 _RS02460 BPHYT_RS02465: BPHYT_RS02465 - taurine ABC transporter permease, at 558,834 to 559,703 _RS02465 BPHYT_RS02470: BPHYT_RS02470 - phosphoserine phosphatase, at 559,731 to 560,414 _RS02470 BPHYT_RS02475: BPHYT_RS02475 - membrane protein, at 560,633 to 562,300 _RS02475 Position (kb) 558 559 560Strain fitness (log2 ratio) -2 -1 0 1at 558.129 kb on - strand, within BPHYT_RS02460at 558.133 kb on - strand, within BPHYT_RS02460at 558.242 kb on + strand, within BPHYT_RS02460at 558.442 kb on + strand, within BPHYT_RS02460at 558.442 kb on + strand, within BPHYT_RS02460at 558.442 kb on + strand, within BPHYT_RS02460at 558.515 kb on + strand, within BPHYT_RS02460at 558.696 kb on + strand, within BPHYT_RS02460at 558.700 kb on + strand, within BPHYT_RS02460at 558.700 kb on + strand, within BPHYT_RS02460at 558.700 kb on + strand, within BPHYT_RS02460at 558.701 kb on - strand, within BPHYT_RS02460at 558.701 kb on - strand, within BPHYT_RS02460at 558.701 kb on - strand, within BPHYT_RS02460at 559.028 kb on - strand, within BPHYT_RS02465at 559.243 kb on - strand, within BPHYT_RS02465at 559.326 kb on + strand, within BPHYT_RS02465at 559.326 kb on + strand, within BPHYT_RS02465at 559.326 kb on + strand, within BPHYT_RS02465at 559.327 kb on - strand, within BPHYT_RS02465at 559.327 kb on - strand, within BPHYT_RS02465at 559.407 kb on + strand, within BPHYT_RS02465at 559.408 kb on - strand, within BPHYT_RS02465at 559.702 kb on - strandat 559.971 kb on - strandat 560.230 kb on + strand, within BPHYT_RS02470at 560.269 kb on + strand, within BPHYT_RS02470at 560.281 kb on + strand, within BPHYT_RS02470at 560.282 kb on - strand, within BPHYT_RS02470at 560.395 kb on - strandat 560.431 kb on + strandat 560.431 kb on + strandat 560.510 kb on + strandat 560.554 kb on + strandat 560.555 kb on - strandat 560.562 kb on + strandat 560.562 kb on + strandat 560.563 kb on - strandat 560.632 kb on + strandat 560.633 kb on - strandat 560.633 kb on - strandat 560.646 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
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558,129 - BPHYT_RS02460 0.11 +1.1
558,133 - BPHYT_RS02460 0.12 +1.1
558,242 + BPHYT_RS02460 0.25 +1.4
558,442 + BPHYT_RS02460 0.50 +1.5
558,442 + BPHYT_RS02460 0.50 +0.0
558,442 + BPHYT_RS02460 0.50 -0.1
558,515 + BPHYT_RS02460 0.60 -2.4
558,696 + BPHYT_RS02460 0.82 +1.3
558,700 + BPHYT_RS02460 0.83 -0.8
558,700 + BPHYT_RS02460 0.83 +0.4
558,700 + BPHYT_RS02460 0.83 -0.4
558,701 - BPHYT_RS02460 0.83 -0.9
558,701 - BPHYT_RS02460 0.83 -0.7
558,701 - BPHYT_RS02460 0.83 +0.2
559,028 - BPHYT_RS02465 0.22 -0.1
559,243 - BPHYT_RS02465 0.47 +0.5
559,326 + BPHYT_RS02465 0.57 +0.3
559,326 + BPHYT_RS02465 0.57 +0.1
559,326 + BPHYT_RS02465 0.57 +1.7
559,327 - BPHYT_RS02465 0.57 +0.7
559,327 - BPHYT_RS02465 0.57 -0.1
559,407 + BPHYT_RS02465 0.66 -0.1
559,408 - BPHYT_RS02465 0.66 +0.2
559,702 - +0.9
559,971 - -1.6
560,230 + BPHYT_RS02470 0.73 +0.1
560,269 + BPHYT_RS02470 0.79 -1.6
560,281 + BPHYT_RS02470 0.80 +0.4
560,282 - BPHYT_RS02470 0.81 -0.8
560,395 - +0.2
560,431 + +0.5
560,431 + -0.2
560,510 + +0.2
560,554 + -0.3
560,555 - -0.1
560,562 + +0.6
560,562 + -1.0
560,563 - +0.3
560,632 + -0.6
560,633 - +0.6
560,633 - +0.8
560,646 + -0.5

Or see this region's nucleotide sequence