Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02255 and BPHYT_RS02260 are separated by 3 nucleotides BPHYT_RS02260 and BPHYT_RS02265 are separated by 243 nucleotides BPHYT_RS02265 and BPHYT_RS02270 are separated by 266 nucleotides
BPHYT_RS02255: BPHYT_RS02255 - hypothetical protein, at 517,824 to 518,288
_RS02255
BPHYT_RS02260: BPHYT_RS02260 - hypothetical protein, at 518,292 to 518,660
_RS02260
BPHYT_RS02265: BPHYT_RS02265 - peptide ABC transporter substrate-binding protein, at 518,904 to 520,490
_RS02265
BPHYT_RS02270: BPHYT_RS02270 - periplasmic binding protein, at 520,757 to 521,575
_RS02270
Position (kb) 518
519
520
521 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 518.003 kb on - strand, within BPHYT_RS02255 at 518.054 kb on + strand, within BPHYT_RS02255 at 518.056 kb on + strand, within BPHYT_RS02255 at 518.057 kb on - strand, within BPHYT_RS02255 at 518.091 kb on + strand, within BPHYT_RS02255 at 518.101 kb on + strand, within BPHYT_RS02255 at 518.101 kb on + strand, within BPHYT_RS02255 at 518.102 kb on - strand, within BPHYT_RS02255 at 518.162 kb on + strand at 518.162 kb on + strand, within BPHYT_RS02255 at 518.165 kb on - strand, within BPHYT_RS02255 at 518.165 kb on - strand, within BPHYT_RS02255 at 518.265 kb on - strand at 518.359 kb on + strand, within BPHYT_RS02260 at 518.360 kb on - strand, within BPHYT_RS02260 at 518.399 kb on - strand, within BPHYT_RS02260 at 518.443 kb on - strand, within BPHYT_RS02260 at 518.479 kb on - strand, within BPHYT_RS02260 at 518.485 kb on + strand, within BPHYT_RS02260 at 518.485 kb on + strand, within BPHYT_RS02260 at 518.515 kb on + strand, within BPHYT_RS02260 at 518.531 kb on - strand, within BPHYT_RS02260 at 518.646 kb on - strand at 519.185 kb on - strand, within BPHYT_RS02265 at 519.547 kb on + strand, within BPHYT_RS02265 at 519.548 kb on - strand, within BPHYT_RS02265 at 519.835 kb on + strand, within BPHYT_RS02265 at 519.857 kb on + strand, within BPHYT_RS02265 at 519.857 kb on + strand, within BPHYT_RS02265 at 519.858 kb on - strand, within BPHYT_RS02265 at 519.859 kb on + strand, within BPHYT_RS02265 at 519.860 kb on - strand, within BPHYT_RS02265 at 519.943 kb on + strand, within BPHYT_RS02265 at 519.944 kb on - strand, within BPHYT_RS02265 at 519.955 kb on + strand, within BPHYT_RS02265 at 519.955 kb on + strand, within BPHYT_RS02265 at 520.243 kb on + strand, within BPHYT_RS02265 at 520.315 kb on + strand, within BPHYT_RS02265 at 520.316 kb on - strand, within BPHYT_RS02265 at 520.461 kb on + strand at 520.461 kb on + strand at 520.462 kb on - strand at 520.540 kb on + strand at 520.599 kb on - strand at 520.599 kb on - strand at 520.665 kb on + strand at 520.695 kb on + strand at 520.785 kb on + strand at 521.060 kb on - strand, within BPHYT_RS02270 at 521.060 kb on - strand, within BPHYT_RS02270 at 521.062 kb on - strand, within BPHYT_RS02270 at 521.106 kb on + strand, within BPHYT_RS02270 at 521.106 kb on + strand, within BPHYT_RS02270 at 521.106 kb on + strand, within BPHYT_RS02270 at 521.223 kb on + strand, within BPHYT_RS02270 at 521.224 kb on - strand, within BPHYT_RS02270 at 521.270 kb on - strand, within BPHYT_RS02270 at 521.450 kb on + strand, within BPHYT_RS02270 at 521.450 kb on + strand, within BPHYT_RS02270
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 518,003 - BPHYT_RS02255 0.38 +0.5 518,054 + BPHYT_RS02255 0.49 -0.2 518,056 + BPHYT_RS02255 0.50 +0.4 518,057 - BPHYT_RS02255 0.50 +0.2 518,091 + BPHYT_RS02255 0.57 +0.6 518,101 + BPHYT_RS02255 0.60 +0.9 518,101 + BPHYT_RS02255 0.60 -0.3 518,102 - BPHYT_RS02255 0.60 -0.0 518,162 + +1.5 518,162 + BPHYT_RS02255 0.73 +2.0 518,165 - BPHYT_RS02255 0.73 +3.7 518,165 - BPHYT_RS02255 0.73 +0.3 518,265 - +0.6 518,359 + BPHYT_RS02260 0.18 +0.2 518,360 - BPHYT_RS02260 0.18 -0.0 518,399 - BPHYT_RS02260 0.29 +0.7 518,443 - BPHYT_RS02260 0.41 +0.1 518,479 - BPHYT_RS02260 0.51 +1.5 518,485 + BPHYT_RS02260 0.52 -0.1 518,485 + BPHYT_RS02260 0.52 +0.6 518,515 + BPHYT_RS02260 0.60 -1.2 518,531 - BPHYT_RS02260 0.65 -0.1 518,646 - -1.1 519,185 - BPHYT_RS02265 0.18 -2.1 519,547 + BPHYT_RS02265 0.41 -2.2 519,548 - BPHYT_RS02265 0.41 +0.0 519,835 + BPHYT_RS02265 0.59 -0.9 519,857 + BPHYT_RS02265 0.60 -0.5 519,857 + BPHYT_RS02265 0.60 +0.9 519,858 - BPHYT_RS02265 0.60 -0.5 519,859 + BPHYT_RS02265 0.60 +1.1 519,860 - BPHYT_RS02265 0.60 -0.6 519,943 + BPHYT_RS02265 0.65 -1.5 519,944 - BPHYT_RS02265 0.66 -0.2 519,955 + BPHYT_RS02265 0.66 -1.5 519,955 + BPHYT_RS02265 0.66 -0.4 520,243 + BPHYT_RS02265 0.84 +0.8 520,315 + BPHYT_RS02265 0.89 +1.1 520,316 - BPHYT_RS02265 0.89 -2.3 520,461 + -0.8 520,461 + -0.2 520,462 - -1.5 520,540 + +0.2 520,599 - +1.6 520,599 - +1.9 520,665 + -1.1 520,695 + +2.0 520,785 + +0.3 521,060 - BPHYT_RS02270 0.37 -0.4 521,060 - BPHYT_RS02270 0.37 +0.5 521,062 - BPHYT_RS02270 0.37 +0.1 521,106 + BPHYT_RS02270 0.43 +0.1 521,106 + BPHYT_RS02270 0.43 +0.7 521,106 + BPHYT_RS02270 0.43 +0.5 521,223 + BPHYT_RS02270 0.57 +0.5 521,224 - BPHYT_RS02270 0.57 +0.1 521,270 - BPHYT_RS02270 0.63 -0.5 521,450 + BPHYT_RS02270 0.85 +0.8 521,450 + BPHYT_RS02270 0.85 -0.2
Or see this region's nucleotide sequence