Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS02175

Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS02170 and BPHYT_RS02175 are separated by 41 nucleotidesBPHYT_RS02175 and BPHYT_RS02180 are separated by 308 nucleotides BPHYT_RS02170: BPHYT_RS02170 - type VI secretion protein, at 497,269 to 499,155 _RS02170 BPHYT_RS02175: BPHYT_RS02175 - type VI secretion protein ImpA, at 499,197 to 501,539 _RS02175 BPHYT_RS02180: BPHYT_RS02180 - hypothetical protein, at 501,848 to 502,372 _RS02180 Position (kb) 499 500 501 502Strain fitness (log2 ratio) -1 0 1 2 3 4 5at 498.584 kb on - strand, within BPHYT_RS02170at 498.757 kb on + strand, within BPHYT_RS02170at 498.757 kb on + strand, within BPHYT_RS02170at 498.779 kb on - strand, within BPHYT_RS02170at 498.847 kb on + strand, within BPHYT_RS02170at 498.847 kb on + strand, within BPHYT_RS02170at 498.848 kb on - strand, within BPHYT_RS02170at 499.164 kb on - strandat 499.276 kb on + strandat 499.317 kb on + strandat 499.324 kb on - strandat 499.655 kb on - strand, within BPHYT_RS02175at 499.865 kb on + strand, within BPHYT_RS02175at 500.077 kb on - strand, within BPHYT_RS02175at 500.091 kb on + strand, within BPHYT_RS02175at 500.343 kb on - strand, within BPHYT_RS02175at 500.371 kb on - strand, within BPHYT_RS02175at 500.662 kb on - strandat 500.685 kb on + strand, within BPHYT_RS02175at 500.686 kb on - strand, within BPHYT_RS02175at 500.702 kb on + strand, within BPHYT_RS02175at 500.703 kb on - strand, within BPHYT_RS02175at 500.988 kb on - strand, within BPHYT_RS02175at 501.572 kb on - strandat 501.667 kb on + strandat 501.667 kb on + strandat 501.746 kb on - strandat 502.019 kb on - strand, within BPHYT_RS02180at 502.178 kb on + strand, within BPHYT_RS02180at 502.261 kb on + strand, within BPHYT_RS02180at 502.410 kb on - strandat 502.516 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
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498,584 - BPHYT_RS02170 0.70 +0.4
498,757 + BPHYT_RS02170 0.79 +0.0
498,757 + BPHYT_RS02170 0.79 +0.0
498,779 - BPHYT_RS02170 0.80 +2.6
498,847 + BPHYT_RS02170 0.84 +0.8
498,847 + BPHYT_RS02170 0.84 -0.5
498,848 - BPHYT_RS02170 0.84 -0.9
499,164 - -0.0
499,276 + +0.1
499,317 + +0.1
499,324 - +0.3
499,655 - BPHYT_RS02175 0.20 -0.1
499,865 + BPHYT_RS02175 0.29 -0.3
500,077 - BPHYT_RS02175 0.38 +0.6
500,091 + BPHYT_RS02175 0.38 -0.3
500,343 - BPHYT_RS02175 0.49 +0.1
500,371 - BPHYT_RS02175 0.50 +0.2
500,662 - -0.7
500,685 + BPHYT_RS02175 0.64 -1.4
500,686 - BPHYT_RS02175 0.64 -0.1
500,702 + BPHYT_RS02175 0.64 -0.3
500,703 - BPHYT_RS02175 0.64 +4.9
500,988 - BPHYT_RS02175 0.76 -0.2
501,572 - -0.3
501,667 + +0.7
501,667 + -0.4
501,746 - -0.8
502,019 - BPHYT_RS02180 0.33 -1.2
502,178 + BPHYT_RS02180 0.63 -1.5
502,261 + BPHYT_RS02180 0.79 +0.7
502,410 - +0.4
502,516 + +0.5

Or see this region's nucleotide sequence