Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02085 and BPHYT_RS02090 are separated by 224 nucleotides BPHYT_RS02090 and BPHYT_RS02095 are separated by 283 nucleotides
BPHYT_RS02085: BPHYT_RS02085 - methylmalonyl-CoA mutase, at 477,797 to 481,693
_RS02085
BPHYT_RS02090: BPHYT_RS02090 - 2-nitropropane dioxygenase, at 481,918 to 482,877
_RS02090
BPHYT_RS02095: BPHYT_RS02095 - alpha/beta hydrolase, at 483,161 to 484,051
_RS02095
Position (kb) 481
482
483 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 480.995 kb on + strand, within BPHYT_RS02085 at 480.996 kb on - strand, within BPHYT_RS02085 at 481.373 kb on + strand at 481.373 kb on + strand at 481.373 kb on + strand at 481.373 kb on + strand at 481.374 kb on - strand at 481.374 kb on - strand at 481.374 kb on - strand at 481.420 kb on + strand at 481.420 kb on + strand at 481.687 kb on + strand at 481.688 kb on - strand at 481.691 kb on + strand at 481.691 kb on + strand at 481.692 kb on - strand at 481.692 kb on - strand at 481.953 kb on + strand at 481.954 kb on - strand at 481.954 kb on - strand at 481.955 kb on + strand at 482.144 kb on + strand, within BPHYT_RS02090 at 482.144 kb on + strand, within BPHYT_RS02090 at 482.145 kb on - strand, within BPHYT_RS02090 at 482.145 kb on - strand, within BPHYT_RS02090 at 482.145 kb on - strand, within BPHYT_RS02090 at 482.175 kb on + strand, within BPHYT_RS02090 at 482.178 kb on - strand, within BPHYT_RS02090 at 482.220 kb on - strand, within BPHYT_RS02090 at 482.270 kb on + strand, within BPHYT_RS02090 at 482.270 kb on + strand, within BPHYT_RS02090 at 482.271 kb on - strand, within BPHYT_RS02090 at 482.271 kb on - strand, within BPHYT_RS02090 at 482.271 kb on - strand, within BPHYT_RS02090 at 482.357 kb on - strand, within BPHYT_RS02090 at 482.357 kb on - strand, within BPHYT_RS02090 at 482.510 kb on + strand, within BPHYT_RS02090 at 482.510 kb on + strand, within BPHYT_RS02090 at 482.511 kb on - strand, within BPHYT_RS02090 at 482.514 kb on + strand, within BPHYT_RS02090 at 482.515 kb on - strand, within BPHYT_RS02090 at 482.877 kb on + strand at 482.877 kb on + strand at 482.877 kb on + strand at 483.078 kb on + strand at 483.079 kb on - strand at 483.079 kb on - strand at 483.079 kb on - strand at 483.348 kb on - strand, within BPHYT_RS02095 at 483.570 kb on - strand, within BPHYT_RS02095 at 483.570 kb on - strand, within BPHYT_RS02095 at 483.570 kb on - strand, within BPHYT_RS02095 at 483.674 kb on + strand, within BPHYT_RS02095 at 483.675 kb on - strand, within BPHYT_RS02095 at 483.675 kb on - strand, within BPHYT_RS02095 at 483.675 kb on - strand, within BPHYT_RS02095 at 483.675 kb on - strand at 483.794 kb on + strand, within BPHYT_RS02095
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 480,995 + BPHYT_RS02085 0.82 +3.3 480,996 - BPHYT_RS02085 0.82 -0.2 481,373 + +0.7 481,373 + -0.4 481,373 + +1.0 481,373 + +0.2 481,374 - +0.0 481,374 - -0.9 481,374 - +0.3 481,420 + -0.5 481,420 + +0.4 481,687 + +0.4 481,688 - -0.2 481,691 + -0.1 481,691 + +0.3 481,692 - -0.3 481,692 - -0.4 481,953 + -0.6 481,954 - -2.1 481,954 - +0.3 481,955 + +0.8 482,144 + BPHYT_RS02090 0.24 +0.8 482,144 + BPHYT_RS02090 0.24 +0.0 482,145 - BPHYT_RS02090 0.24 -0.8 482,145 - BPHYT_RS02090 0.24 -0.4 482,145 - BPHYT_RS02090 0.24 +0.7 482,175 + BPHYT_RS02090 0.27 -0.6 482,178 - BPHYT_RS02090 0.27 +0.1 482,220 - BPHYT_RS02090 0.31 -0.2 482,270 + BPHYT_RS02090 0.37 -0.4 482,270 + BPHYT_RS02090 0.37 +0.3 482,271 - BPHYT_RS02090 0.37 +0.1 482,271 - BPHYT_RS02090 0.37 -0.5 482,271 - BPHYT_RS02090 0.37 -1.3 482,357 - BPHYT_RS02090 0.46 -0.1 482,357 - BPHYT_RS02090 0.46 -2.4 482,510 + BPHYT_RS02090 0.62 -1.1 482,510 + BPHYT_RS02090 0.62 -0.3 482,511 - BPHYT_RS02090 0.62 -0.4 482,514 + BPHYT_RS02090 0.62 +0.3 482,515 - BPHYT_RS02090 0.62 +2.1 482,877 + +0.3 482,877 + -0.8 482,877 + -0.3 483,078 + -0.5 483,079 - +0.0 483,079 - +1.2 483,079 - +0.3 483,348 - BPHYT_RS02095 0.21 +0.8 483,570 - BPHYT_RS02095 0.46 +1.2 483,570 - BPHYT_RS02095 0.46 -0.5 483,570 - BPHYT_RS02095 0.46 -0.8 483,674 + BPHYT_RS02095 0.58 +0.7 483,675 - BPHYT_RS02095 0.58 -2.1 483,675 - BPHYT_RS02095 0.58 -0.6 483,675 - BPHYT_RS02095 0.58 -1.6 483,675 - +0.3 483,794 + BPHYT_RS02095 0.71 +0.7
Or see this region's nucleotide sequence