Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02050 and BPHYT_RS02055 overlap by 4 nucleotides BPHYT_RS02055 and BPHYT_RS02060 are separated by 195 nucleotides
BPHYT_RS02050: BPHYT_RS02050 - D-mannose isomerase (EC 5.3.1.7) (from data) , at 468,044 to 469,327
_RS02050
BPHYT_RS02055: BPHYT_RS02055 - LacI family transcription regulator, at 469,324 to 470,310
_RS02055
BPHYT_RS02060: BPHYT_RS02060 - diguanylate cyclase, at 470,506 to 472,356
_RS02060
Position (kb)
469
470
471 Strain fitness (log2 ratio)
-2
-1
0
1 at 468.342 kb on + strand, within BPHYT_RS02050 at 468.342 kb on + strand, within BPHYT_RS02050 at 468.342 kb on + strand, within BPHYT_RS02050 at 468.342 kb on + strand, within BPHYT_RS02050 at 468.343 kb on - strand, within BPHYT_RS02050 at 468.384 kb on + strand, within BPHYT_RS02050 at 468.385 kb on - strand, within BPHYT_RS02050 at 468.385 kb on - strand, within BPHYT_RS02050 at 468.387 kb on + strand, within BPHYT_RS02050 at 468.388 kb on - strand, within BPHYT_RS02050 at 468.573 kb on + strand, within BPHYT_RS02050 at 468.573 kb on + strand, within BPHYT_RS02050 at 468.652 kb on + strand, within BPHYT_RS02050 at 468.685 kb on - strand, within BPHYT_RS02050 at 468.685 kb on - strand, within BPHYT_RS02050 at 468.990 kb on + strand, within BPHYT_RS02050 at 469.050 kb on + strand, within BPHYT_RS02050 at 469.096 kb on - strand, within BPHYT_RS02050 at 469.096 kb on - strand, within BPHYT_RS02050 at 469.534 kb on + strand, within BPHYT_RS02055 at 470.652 kb on - strand at 470.652 kb on - strand at 470.780 kb on + strand, within BPHYT_RS02060 at 470.963 kb on + strand, within BPHYT_RS02060 at 470.970 kb on - strand, within BPHYT_RS02060 at 471.009 kb on - strand, within BPHYT_RS02060 at 471.017 kb on + strand, within BPHYT_RS02060 at 471.209 kb on + strand, within BPHYT_RS02060
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 468,342 + BPHYT_RS02050 0.23 -1.1 468,342 + BPHYT_RS02050 0.23 -0.5 468,342 + BPHYT_RS02050 0.23 -0.2 468,342 + BPHYT_RS02050 0.23 -1.4 468,343 - BPHYT_RS02050 0.23 -0.9 468,384 + BPHYT_RS02050 0.26 +0.1 468,385 - BPHYT_RS02050 0.27 -0.3 468,385 - BPHYT_RS02050 0.27 -0.1 468,387 + BPHYT_RS02050 0.27 -0.5 468,388 - BPHYT_RS02050 0.27 -0.3 468,573 + BPHYT_RS02050 0.41 -0.6 468,573 + BPHYT_RS02050 0.41 -1.2 468,652 + BPHYT_RS02050 0.47 +0.2 468,685 - BPHYT_RS02050 0.50 +1.6 468,685 - BPHYT_RS02050 0.50 -0.0 468,990 + BPHYT_RS02050 0.74 +1.0 469,050 + BPHYT_RS02050 0.78 -2.6 469,096 - BPHYT_RS02050 0.82 +1.3 469,096 - BPHYT_RS02050 0.82 -0.3 469,534 + BPHYT_RS02055 0.21 +0.3 470,652 - +0.1 470,652 - -0.0 470,780 + BPHYT_RS02060 0.15 +0.2 470,963 + BPHYT_RS02060 0.25 -0.0 470,970 - BPHYT_RS02060 0.25 +0.3 471,009 - BPHYT_RS02060 0.27 -0.7 471,017 + BPHYT_RS02060 0.28 -0.5 471,209 + BPHYT_RS02060 0.38 -0.5
Or see this region's nucleotide sequence